Instantaneous isotropic volumetric imaging of fast biological processes
(2019) In Nature Methods 16(6). p.497-500- Abstract
To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/f73c99c9-e370-4bb2-94f0-2b53ea686dc9
- author
- Wagner, Nils ; Norlin, Nils LU ; Gierten, Jakob ; de Medeiros, Gustavo ; Balázs, Bálint ; Wittbrodt, Joachim ; Hufnagel, Lars and Prevedel, Robert
- publishing date
- 2019-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Algorithms, Animals, Animals, Genetically Modified, Artifacts, Blood Flow Velocity, Heart/diagnostic imaging, Humans, Image Processing, Computer-Assisted/methods, Imaging, Three-Dimensional/methods, Microscopy/methods, Oryzias, Single-Cell Analysis/methods
- in
- Nature Methods
- volume
- 16
- issue
- 6
- pages
- 4 pages
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85065167881
- pmid:31036959
- ISSN
- 1548-7105
- DOI
- 10.1038/s41592-019-0393-z
- language
- English
- LU publication?
- no
- id
- f73c99c9-e370-4bb2-94f0-2b53ea686dc9
- date added to LUP
- 2020-09-08 22:32:48
- date last changed
- 2024-07-10 22:40:53
@article{f73c99c9-e370-4bb2-94f0-2b53ea686dc9, abstract = {{<p>To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.</p>}}, author = {{Wagner, Nils and Norlin, Nils and Gierten, Jakob and de Medeiros, Gustavo and Balázs, Bálint and Wittbrodt, Joachim and Hufnagel, Lars and Prevedel, Robert}}, issn = {{1548-7105}}, keywords = {{Algorithms; Animals; Animals, Genetically Modified; Artifacts; Blood Flow Velocity; Heart/diagnostic imaging; Humans; Image Processing, Computer-Assisted/methods; Imaging, Three-Dimensional/methods; Microscopy/methods; Oryzias; Single-Cell Analysis/methods}}, language = {{eng}}, number = {{6}}, pages = {{497--500}}, publisher = {{Nature Publishing Group}}, series = {{Nature Methods}}, title = {{Instantaneous isotropic volumetric imaging of fast biological processes}}, url = {{http://dx.doi.org/10.1038/s41592-019-0393-z}}, doi = {{10.1038/s41592-019-0393-z}}, volume = {{16}}, year = {{2019}}, }