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Instantaneous isotropic volumetric imaging of fast biological processes

Wagner, Nils ; Norlin, Nils LU ; Gierten, Jakob ; de Medeiros, Gustavo ; Balázs, Bálint ; Wittbrodt, Joachim ; Hufnagel, Lars and Prevedel, Robert (2019) In Nature Methods 16(6). p.497-500
Abstract

To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.

Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Algorithms, Animals, Animals, Genetically Modified, Artifacts, Blood Flow Velocity, Heart/diagnostic imaging, Humans, Image Processing, Computer-Assisted/methods, Imaging, Three-Dimensional/methods, Microscopy/methods, Oryzias, Single-Cell Analysis/methods
in
Nature Methods
volume
16
issue
6
pages
4 pages
publisher
Nature Publishing Group
external identifiers
  • pmid:31036959
  • scopus:85065167881
ISSN
1548-7105
DOI
10.1038/s41592-019-0393-z
language
English
LU publication?
no
id
f73c99c9-e370-4bb2-94f0-2b53ea686dc9
date added to LUP
2020-09-08 22:32:48
date last changed
2024-06-13 23:47:41
@article{f73c99c9-e370-4bb2-94f0-2b53ea686dc9,
  abstract     = {{<p>To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a significant reduction of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate medaka heart and blood flow imaging at single-cell resolution and free of motion artefacts at volume rates of up to 200 Hz.</p>}},
  author       = {{Wagner, Nils and Norlin, Nils and Gierten, Jakob and de Medeiros, Gustavo and Balázs, Bálint and Wittbrodt, Joachim and Hufnagel, Lars and Prevedel, Robert}},
  issn         = {{1548-7105}},
  keywords     = {{Algorithms; Animals; Animals, Genetically Modified; Artifacts; Blood Flow Velocity; Heart/diagnostic imaging; Humans; Image Processing, Computer-Assisted/methods; Imaging, Three-Dimensional/methods; Microscopy/methods; Oryzias; Single-Cell Analysis/methods}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{497--500}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Methods}},
  title        = {{Instantaneous isotropic volumetric imaging of fast biological processes}},
  url          = {{http://dx.doi.org/10.1038/s41592-019-0393-z}},
  doi          = {{10.1038/s41592-019-0393-z}},
  volume       = {{16}},
  year         = {{2019}},
}