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Epithelial permeability to proteins in the noninflamed ileum of Crohn's disease?

Soderholm, J. D. ; Holmgren Peterson, Kajsa ; Olaison, G. ; Franzén, Lennart E. ; Westrom, B. LU ; Magnusson, K. E. and Sjodahl, Rune (1999) In Gastroenterology 117(1). p.65-72
Abstract

Background and Aims: Crohn's disease (CD) is associated with a disturbed intestinal barrier. Permeability studies have focused on inert molecules, but little is known about transepithelial transport of macromolecules with antigenic potential in humans. The aim of this study was to quantify permeation and to characterize passage routes for macromolecules in ileal mucosa in CD. Methods: Noninflamed and inflamed ileal mucosa specimens from patients with CD (n = 12) and ileal specimens from patients with colon cancer (n = 7) were studied regarding transmucosal permeation of ovalbumin, dextran (mol wt, 40,000), and 51Cr-EDTA for 90 minutes in vitro in Ussing chambers. Transepithelial passage routes for fluorescent ovalbumin and... (More)

Background and Aims: Crohn's disease (CD) is associated with a disturbed intestinal barrier. Permeability studies have focused on inert molecules, but little is known about transepithelial transport of macromolecules with antigenic potential in humans. The aim of this study was to quantify permeation and to characterize passage routes for macromolecules in ileal mucosa in CD. Methods: Noninflamed and inflamed ileal mucosa specimens from patients with CD (n = 12) and ileal specimens from patients with colon cancer (n = 7) were studied regarding transmucosal permeation of ovalbumin, dextran (mol wt, 40,000), and 51Cr-EDTA for 90 minutes in vitro in Ussing chambers. Transepithelial passage routes for fluorescent ovalbumin and dextran 40,000 were investigated by confocal microscopy. Results: Noninflamed ileum from CD patients showed increased permeation of ovalbumin compared with ileum from colon cancer patients (P < 0.05). Dextran permeation was equal in the three groups, whereas 51Cr-EDTA permeability was increased in inflamed ileum. Ovalbumin passed both transcellularly and paracellularly, but dextran followed a strictly paracellular route. Both markers were subsequently endocytosed by cells of the lamina propria. Conclusions: Noninflamed ileal mucosa from patients with CD shows increased epithelial permeability to ovalbumin, probably by augmented transcytosis. This increase in antigen load to the lamina propria could be an initiating pathogenic event in CD.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Gastroenterology
volume
117
issue
1
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:0032991667
  • pmid:10381911
ISSN
0016-5085
DOI
10.1016/S0016-5085(99)70551-2
language
English
LU publication?
yes
id
f75f676a-f27c-4efc-9953-4fec421d5e24
date added to LUP
2024-12-05 15:16:13
date last changed
2025-06-06 18:41:56
@article{f75f676a-f27c-4efc-9953-4fec421d5e24,
  abstract     = {{<p>Background and Aims: Crohn's disease (CD) is associated with a disturbed intestinal barrier. Permeability studies have focused on inert molecules, but little is known about transepithelial transport of macromolecules with antigenic potential in humans. The aim of this study was to quantify permeation and to characterize passage routes for macromolecules in ileal mucosa in CD. Methods: Noninflamed and inflamed ileal mucosa specimens from patients with CD (n = 12) and ileal specimens from patients with colon cancer (n = 7) were studied regarding transmucosal permeation of ovalbumin, dextran (mol wt, 40,000), and <sup>51</sup>Cr-EDTA for 90 minutes in vitro in Ussing chambers. Transepithelial passage routes for fluorescent ovalbumin and dextran 40,000 were investigated by confocal microscopy. Results: Noninflamed ileum from CD patients showed increased permeation of ovalbumin compared with ileum from colon cancer patients (P &lt; 0.05). Dextran permeation was equal in the three groups, whereas <sup>51</sup>Cr-EDTA permeability was increased in inflamed ileum. Ovalbumin passed both transcellularly and paracellularly, but dextran followed a strictly paracellular route. Both markers were subsequently endocytosed by cells of the lamina propria. Conclusions: Noninflamed ileal mucosa from patients with CD shows increased epithelial permeability to ovalbumin, probably by augmented transcytosis. This increase in antigen load to the lamina propria could be an initiating pathogenic event in CD.</p>}},
  author       = {{Soderholm, J. D. and Holmgren Peterson, Kajsa and Olaison, G. and Franzén, Lennart E. and Westrom, B. and Magnusson, K. E. and Sjodahl, Rune}},
  issn         = {{0016-5085}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{65--72}},
  publisher    = {{Elsevier}},
  series       = {{Gastroenterology}},
  title        = {{Epithelial permeability to proteins in the noninflamed ileum of Crohn's disease?}},
  url          = {{http://dx.doi.org/10.1016/S0016-5085(99)70551-2}},
  doi          = {{10.1016/S0016-5085(99)70551-2}},
  volume       = {{117}},
  year         = {{1999}},
}