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Immobilisation of DNA to polymerised SU-8 photoresist

Marie, Rodolphe ; Schmid, Silvan ; Johansson, Alicia ; Ejsing, Louise ; Nordström, Maria LU ; Häfliger, Daniel ; Christensen, Claus BV ; Boisen, Anja and Dufva, Martin (2006) In Biosensors & Bioelectronics 21(7). p.1327-1332
Abstract
SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalise SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm2 as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture probes was measured on commercial aldehyde coated glass. DNA probes did not decrease in hybridisation performance after 10 min incubation in water at 98 °C prior to hybridisation, indicating a covalent bond between DNA and SU-8. Finally, DNA... (More)
SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalise SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm2 as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture probes was measured on commercial aldehyde coated glass. DNA probes did not decrease in hybridisation performance after 10 min incubation in water at 98 °C prior to hybridisation, indicating a covalent bond between DNA and SU-8. Finally, DNA microarrays of high quality were obtained on SU-8 by contact printing of probe solution directly on SU-8 demonstrating a simple method for the implementation of microarrays in microsystems. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Biosensors & Bioelectronics
volume
21
issue
7
pages
1327 - 1332
publisher
Elsevier
external identifiers
  • scopus:29144452883
ISSN
1873-4235
DOI
10.1016/j.bios.2005.03.004
language
English
LU publication?
no
id
f7a21e43-f6e7-4439-8287-245df9389a19 (old id 3157952)
date added to LUP
2016-04-01 16:51:48
date last changed
2022-01-28 22:39:31
@article{f7a21e43-f6e7-4439-8287-245df9389a19,
  abstract     = {{SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalise SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm2 as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture probes was measured on commercial aldehyde coated glass. DNA probes did not decrease in hybridisation performance after 10 min incubation in water at 98 °C prior to hybridisation, indicating a covalent bond between DNA and SU-8. Finally, DNA microarrays of high quality were obtained on SU-8 by contact printing of probe solution directly on SU-8 demonstrating a simple method for the implementation of microarrays in microsystems.}},
  author       = {{Marie, Rodolphe and Schmid, Silvan and Johansson, Alicia and Ejsing, Louise and Nordström, Maria and Häfliger, Daniel and Christensen, Claus BV and Boisen, Anja and Dufva, Martin}},
  issn         = {{1873-4235}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{1327--1332}},
  publisher    = {{Elsevier}},
  series       = {{Biosensors & Bioelectronics}},
  title        = {{Immobilisation of DNA to polymerised SU-8 photoresist}},
  url          = {{http://dx.doi.org/10.1016/j.bios.2005.03.004}},
  doi          = {{10.1016/j.bios.2005.03.004}},
  volume       = {{21}},
  year         = {{2006}},
}