Advanced

Microcontact imprinted plasmonic nanosensors : Powerful tools in the detection of salmonella paratyphi

Perçin, Isık; Idil, Neslihan; Bakhshpour, Monireh; Yılmaz, Erkut; Mattiasson, Bo LU and Denizli, Adil (2017) In Sensors 17(6).
Abstract

Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 ×... (More)

Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 × 106-15 × 106 CFU/mL. Selectivity of the prepared sensors was examined by using competing bacterial strains such as Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The imprinting efficiency of the prepared sensor system was determined by evaluating the responses of the SPR chips prepared with both molecularly imprinted polymers (MIPs) and non-imprinted polymers (NIPs). Real sample experiments were performed with apple juice. The recognition of Salmonella paratyphi was achieved using these SPR sensor with a detection limit of 1.4 × 106 CFU/mL. In conclusion, SPR sensor has the potential to serve as an excellent candidate for monitoring Salmonella paratyphi in food supplies or contaminated water and clearly makes it possible to develop rapid and appropriate control strategies.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Microcontact imprinting, N-methacryloyl-L-histidine methyl ester, Salmonella paratyphi, SPR biosensor
in
Sensors
volume
17
issue
6
publisher
MDPI AG
external identifiers
  • scopus:85020943648
  • wos:000404553900191
ISSN
1424-8220
DOI
10.3390/s17061375
language
English
LU publication?
yes
id
f7b15a0a-b833-4c52-95c0-6b4b8eb6b5a5
date added to LUP
2017-07-04 09:31:52
date last changed
2017-09-18 11:37:37
@article{f7b15a0a-b833-4c52-95c0-6b4b8eb6b5a5,
  abstract     = {<p>Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH). Characterization studies of the SPR chips were carried out with ellipsometry and scanning electron microscopy (SEM). The real-time Salmonella paratyphi detection was performed within the range of 2.5 × 10<sup>6</sup>-15 × 10<sup>6</sup> CFU/mL. Selectivity of the prepared sensors was examined by using competing bacterial strains such as Escherichia coli, Staphylococcus aureus and Bacillus subtilis. The imprinting efficiency of the prepared sensor system was determined by evaluating the responses of the SPR chips prepared with both molecularly imprinted polymers (MIPs) and non-imprinted polymers (NIPs). Real sample experiments were performed with apple juice. The recognition of Salmonella paratyphi was achieved using these SPR sensor with a detection limit of 1.4 × 10<sup>6</sup> CFU/mL. In conclusion, SPR sensor has the potential to serve as an excellent candidate for monitoring Salmonella paratyphi in food supplies or contaminated water and clearly makes it possible to develop rapid and appropriate control strategies.</p>},
  articleno    = {1375},
  author       = {Perçin, Isık and Idil, Neslihan and Bakhshpour, Monireh and Yılmaz, Erkut and Mattiasson, Bo and Denizli, Adil},
  issn         = {1424-8220},
  keyword      = {Microcontact imprinting,N-methacryloyl-L-histidine methyl ester,Salmonella paratyphi,SPR biosensor},
  language     = {eng},
  month        = {06},
  number       = {6},
  publisher    = {MDPI AG},
  series       = {Sensors},
  title        = {Microcontact imprinted plasmonic nanosensors : Powerful tools in the detection of salmonella paratyphi},
  url          = {http://dx.doi.org/10.3390/s17061375},
  volume       = {17},
  year         = {2017},
}