Oxidative stress decreases antioxidant enzyme activities in reaggregation cultures of rat brain cells
Aspberg, Anders LU
and Tottmar, Olof
(1994)
In Free Radical Biology & Medicine
17(6).
p.511-516
- Abstract
- The brain has been suggested to be especially sensitive to damage by reactive oxygen species. In this study, we examined the effects of hyperoxic conditions on the activities and mRNA levels of antioxidant enzymes in reaggregation cultures of rat forebrain cells. Cultures were exposed to 80% oxygen for 12-60 h starting on Days 17 and 33 in culture. Superoxide dismutase activities and mRNA levels were not affected by hyperoxia, whereas catalase activity was slightly decreased after 24 h in 80% oxygen at Day 17. Glutathione peroxidase activity was markedly decreased already after 12 h of hyperoxia, and decreased activities of glutathione reductase and glucose-6-phosphate dehydrogenase were also noted. The glutathione peroxidase mRNA levels... (More)
- The brain has been suggested to be especially sensitive to damage by reactive oxygen species. In this study, we examined the effects of hyperoxic conditions on the activities and mRNA levels of antioxidant enzymes in reaggregation cultures of rat forebrain cells. Cultures were exposed to 80% oxygen for 12-60 h starting on Days 17 and 33 in culture. Superoxide dismutase activities and mRNA levels were not affected by hyperoxia, whereas catalase activity was slightly decreased after 24 h in 80% oxygen at Day 17. Glutathione peroxidase activity was markedly decreased already after 12 h of hyperoxia, and decreased activities of glutathione reductase and glucose-6-phosphate dehydrogenase were also noted. The glutathione peroxidase mRNA levels were increased in hyperoxic cultures at Day 17 but not at Day 33. These results suggest that the enzymatic defense mechanisms against reactive oxygen species in the brain are rather weak and deteriorate during oxidative stress but that a potential for compensatory upregulation exists at least during the first postnatal weeks. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1108637
- author
- Aspberg, Anders
LU
and Tottmar, Olof
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Superoxide dismutase, Catalase, Glutathione peroxidase, Oxidative stress, Rat brain, Reaggregation cultures, Free radicals
- in
- Free Radical Biology & Medicine
- volume
- 17
- issue
- 6
- pages
- 511 - 516
- publisher
- Elsevier
- external identifiers
-
- pmid:7867967
- scopus:0028046390
- ISSN
- 0891-5849
- DOI
- 10.1016/0891-5849(94)90090-6
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151)
- id
- f7b3ad94-67ec-4079-a118-d1c69a989f06 (old id 1108637)
- date added to LUP
- 2016-04-01 12:35:15
- date last changed
- 2021-03-21 05:50:02
@article{f7b3ad94-67ec-4079-a118-d1c69a989f06,
abstract = {{The brain has been suggested to be especially sensitive to damage by reactive oxygen species. In this study, we examined the effects of hyperoxic conditions on the activities and mRNA levels of antioxidant enzymes in reaggregation cultures of rat forebrain cells. Cultures were exposed to 80% oxygen for 12-60 h starting on Days 17 and 33 in culture. Superoxide dismutase activities and mRNA levels were not affected by hyperoxia, whereas catalase activity was slightly decreased after 24 h in 80% oxygen at Day 17. Glutathione peroxidase activity was markedly decreased already after 12 h of hyperoxia, and decreased activities of glutathione reductase and glucose-6-phosphate dehydrogenase were also noted. The glutathione peroxidase mRNA levels were increased in hyperoxic cultures at Day 17 but not at Day 33. These results suggest that the enzymatic defense mechanisms against reactive oxygen species in the brain are rather weak and deteriorate during oxidative stress but that a potential for compensatory upregulation exists at least during the first postnatal weeks.}},
author = {{Aspberg, Anders and Tottmar, Olof}},
issn = {{0891-5849}},
keywords = {{Superoxide dismutase; Catalase; Glutathione peroxidase; Oxidative stress; Rat brain; Reaggregation cultures; Free radicals}},
language = {{eng}},
number = {{6}},
pages = {{511--516}},
publisher = {{Elsevier}},
series = {{Free Radical Biology & Medicine}},
title = {{Oxidative stress decreases antioxidant enzyme activities in reaggregation cultures of rat brain cells}},
url = {{http://dx.doi.org/10.1016/0891-5849(94)90090-6}},
doi = {{10.1016/0891-5849(94)90090-6}},
volume = {{17}},
year = {{1994}},
}