Early attachment of platelets, leukocytes, and fibrinogen in endothelial cell seeded Dacron grafts
(1996) In Annals of Vascular Surgery 10(6). p.530-536- Abstract
- Endothelial cell seeding has been advocated as a method for reducing the thrombogenicity of prosthetic grafts. Principally two different techniques for endothelial cell seeding can be used: immediate seeding of grafts followed by implantation or initial growth and establishment of an endothelial cell-covered surface before subsequent late implantation. This study was designed to determine whether the immediate seeding technique altered thrombogenicity directly after graft implantation. Carotid arteries from 19 sheep were replaced with Dacron interposition grafts; one side was seeded with endothelial cells and the other side was left unseeded. The dynamics of thrombus formation involving radiolabeled platelets, leukocytes, and fibrinogen... (More)
- Endothelial cell seeding has been advocated as a method for reducing the thrombogenicity of prosthetic grafts. Principally two different techniques for endothelial cell seeding can be used: immediate seeding of grafts followed by implantation or initial growth and establishment of an endothelial cell-covered surface before subsequent late implantation. This study was designed to determine whether the immediate seeding technique altered thrombogenicity directly after graft implantation. Carotid arteries from 19 sheep were replaced with Dacron interposition grafts; one side was seeded with endothelial cells and the other side was left unseeded. The dynamics of thrombus formation involving radiolabeled platelets, leukocytes, and fibrinogen were studied for 4 hours with flow reduced to 35 ml/min. No difference in platelet uptake (approximately 6-fold increase compared to baseline values) was found between endothelial cell seeded and unseeded grafts. Likewise, there were no differences in leukocyte uptake (approximately 4-fold increase) or fibrinogen uptake (approximately 10- to 15-fold increase) between the two groups. No differences were demonstrated with regard to patency or thrombus weight. In this experimental investigation we were unable to verify any change in the uptake of platelets, white blood cells, or fibrinogen between endothelial cell seeded and unseeded Dacron grafts during the first 4 hours after graft placement. Immediate seeding does not affect the initial thrombogenicity of grafts. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1110797
- author
- Jensen, Norman ; Lindblad, Bengt LU ; Ljungberg, June LU ; Leide, Sigrid and Bergqvist, David
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Annals of Vascular Surgery
- volume
- 10
- issue
- 6
- pages
- 530 - 536
- publisher
- Springer
- external identifiers
-
- pmid:8989969
- scopus:0030468711
- ISSN
- 1615-5947
- DOI
- 10.1007/BF02000441
- language
- English
- LU publication?
- yes
- id
- f7ba060e-3ad0-4991-9c36-30623a49f1d0 (old id 1110797)
- date added to LUP
- 2016-04-01 12:20:58
- date last changed
- 2024-01-08 17:25:55
@article{f7ba060e-3ad0-4991-9c36-30623a49f1d0,
abstract = {{Endothelial cell seeding has been advocated as a method for reducing the thrombogenicity of prosthetic grafts. Principally two different techniques for endothelial cell seeding can be used: immediate seeding of grafts followed by implantation or initial growth and establishment of an endothelial cell-covered surface before subsequent late implantation. This study was designed to determine whether the immediate seeding technique altered thrombogenicity directly after graft implantation. Carotid arteries from 19 sheep were replaced with Dacron interposition grafts; one side was seeded with endothelial cells and the other side was left unseeded. The dynamics of thrombus formation involving radiolabeled platelets, leukocytes, and fibrinogen were studied for 4 hours with flow reduced to 35 ml/min. No difference in platelet uptake (approximately 6-fold increase compared to baseline values) was found between endothelial cell seeded and unseeded grafts. Likewise, there were no differences in leukocyte uptake (approximately 4-fold increase) or fibrinogen uptake (approximately 10- to 15-fold increase) between the two groups. No differences were demonstrated with regard to patency or thrombus weight. In this experimental investigation we were unable to verify any change in the uptake of platelets, white blood cells, or fibrinogen between endothelial cell seeded and unseeded Dacron grafts during the first 4 hours after graft placement. Immediate seeding does not affect the initial thrombogenicity of grafts.}},
author = {{Jensen, Norman and Lindblad, Bengt and Ljungberg, June and Leide, Sigrid and Bergqvist, David}},
issn = {{1615-5947}},
language = {{eng}},
number = {{6}},
pages = {{530--536}},
publisher = {{Springer}},
series = {{Annals of Vascular Surgery}},
title = {{Early attachment of platelets, leukocytes, and fibrinogen in endothelial cell seeded Dacron grafts}},
url = {{http://dx.doi.org/10.1007/BF02000441}},
doi = {{10.1007/BF02000441}},
volume = {{10}},
year = {{1996}},
}