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Presence of vitronectin and activated complement factor C9 on ventriculoperitoneal shunts and temporary ventricular drainage catheters

Lundberg, Fredrik LU ; Li, Dai Qing ; Falkenback, Dan ; Lea, Tor ; Siesjö, Peter LU ; Söderström, Sven ; Kudryk, Bohdan J. ; Tegenfeldt, Jonas O. LU ; Nomura, Sadahiro and Ljungh, Åsa (1999) In Journal of Neurosurgery 90(1). p.101-108
Abstract

Object. The pathogenesis of cerebrospinal fluid (CSF) shunt infection is characterized by staphylococcal adhesion to the polymeric surface of the shunt catheter. Proteins from the CSF-fibronectin, vitronectin, and fibrinogen-are adsorbed to the surface of the catheter immediately after insertion. These proteins can interfere with the biological systems of the host and mediate staphylococcal adhesion to the surface of the catheter. In the present study, the presence of fibronectin, vitronectin, and fibrinogen on CSF shunts and temporary ventricular drainage catheters is shown. The presence of fragments of fibrinogen is also examined. Methods. The authors used the following methods: binding radiolabeled antibodies to the catheter surface,... (More)

Object. The pathogenesis of cerebrospinal fluid (CSF) shunt infection is characterized by staphylococcal adhesion to the polymeric surface of the shunt catheter. Proteins from the CSF-fibronectin, vitronectin, and fibrinogen-are adsorbed to the surface of the catheter immediately after insertion. These proteins can interfere with the biological systems of the host and mediate staphylococcal adhesion to the surface of the catheter. In the present study, the presence of fibronectin, vitronectin, and fibrinogen on CSF shunts and temporary ventricular drainage catheters is shown. The presence of fragments of fibrinogen is also examined. Methods. The authors used the following methods: binding radiolabeled antibodies to the catheter surface, immunoblotting of catheter eluates, and scanning force microscopy of immunogold bound to the catheter surface. The immunoblot showed that vitronectin was adsorbed in its native form and that fibronectin was degraded into small fragments. Furthermore, the study demonstrated that the level of vitronectin in CSF increased in patients with an impaired CSF-blood barrier. To study complement activation, an antibody that recognizes the neoepitope of activated complement factor C9 was used. The presence of activated complement factor C9 was shown on both temporary catheters and shunts. Conclusions. Activation of complement close to the surface of an inserted catheter could contribute to the pathogenesis of CSF shunt infection.

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; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Activated complement C9, Fibrinogen, Immunogold, Scanning force microscopy, Ventricular catheter, Vitronectin
in
Journal of Neurosurgery
volume
90
issue
1
pages
8 pages
publisher
American Association of Neurosurgeons
external identifiers
  • pmid:10413162
  • scopus:0032948821
ISSN
0022-3085
DOI
10.3171/jns.1999.90.1.0101
language
English
LU publication?
yes
id
f7ce07da-6ae2-48a6-b54b-b945e85e5c25
date added to LUP
2018-10-20 10:51:39
date last changed
2020-01-13 01:04:54
@article{f7ce07da-6ae2-48a6-b54b-b945e85e5c25,
  abstract     = {<p>Object. The pathogenesis of cerebrospinal fluid (CSF) shunt infection is characterized by staphylococcal adhesion to the polymeric surface of the shunt catheter. Proteins from the CSF-fibronectin, vitronectin, and fibrinogen-are adsorbed to the surface of the catheter immediately after insertion. These proteins can interfere with the biological systems of the host and mediate staphylococcal adhesion to the surface of the catheter. In the present study, the presence of fibronectin, vitronectin, and fibrinogen on CSF shunts and temporary ventricular drainage catheters is shown. The presence of fragments of fibrinogen is also examined. Methods. The authors used the following methods: binding radiolabeled antibodies to the catheter surface, immunoblotting of catheter eluates, and scanning force microscopy of immunogold bound to the catheter surface. The immunoblot showed that vitronectin was adsorbed in its native form and that fibronectin was degraded into small fragments. Furthermore, the study demonstrated that the level of vitronectin in CSF increased in patients with an impaired CSF-blood barrier. To study complement activation, an antibody that recognizes the neoepitope of activated complement factor C9 was used. The presence of activated complement factor C9 was shown on both temporary catheters and shunts. Conclusions. Activation of complement close to the surface of an inserted catheter could contribute to the pathogenesis of CSF shunt infection.</p>},
  author       = {Lundberg, Fredrik and Li, Dai Qing and Falkenback, Dan and Lea, Tor and Siesjö, Peter and Söderström, Sven and Kudryk, Bohdan J. and Tegenfeldt, Jonas O. and Nomura, Sadahiro and Ljungh, Åsa},
  issn         = {0022-3085},
  language     = {eng},
  month        = {01},
  number       = {1},
  pages        = {101--108},
  publisher    = {American Association of Neurosurgeons},
  series       = {Journal of Neurosurgery},
  title        = {Presence of vitronectin and activated complement factor C9 on ventriculoperitoneal shunts and temporary ventricular drainage catheters},
  url          = {http://dx.doi.org/10.3171/jns.1999.90.1.0101},
  doi          = {10.3171/jns.1999.90.1.0101},
  volume       = {90},
  year         = {1999},
}