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Differential Expression of Klotho in the Brain and Spinal Cord is Associated with Total Antioxidant Capacity in Mice with Experimental Autoimmune Encephalomyelitis

Emami Aleagha, Mohammad Sajad; Harirchian, Mohammad Hossein; Lavasani, Shahram LU ; Javan, Mohammad and Allameh, Abdolamir (2018) In Journal of Molecular Neuroscience 64(4). p.543-550
Abstract

Recently, we reported a positive correlation between Klotho, as an anti-aging protein, and the total antioxidant capacity (TAC) in cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients. However, there is no information about the Klotho and TAC changes within the central nervous system (CNS). Thus, the current study aimed to employ an experimental autoimmune encephalomyelitis (EAE) model in C57BL/6 mice using MOG35–55 peptide to examine the relationship between Klotho and TAC within the CNS. To this end, the brain and spinal cord were obtained at the onset and peak stages of EAE as well as non-EAE mice (sham/control groups). The Klotho expression was assessed in the brain and spinal cord of different experimental... (More)

Recently, we reported a positive correlation between Klotho, as an anti-aging protein, and the total antioxidant capacity (TAC) in cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients. However, there is no information about the Klotho and TAC changes within the central nervous system (CNS). Thus, the current study aimed to employ an experimental autoimmune encephalomyelitis (EAE) model in C57BL/6 mice using MOG35–55 peptide to examine the relationship between Klotho and TAC within the CNS. To this end, the brain and spinal cord were obtained at the onset and peak stages of EAE as well as non-EAE mice (sham/control groups). The Klotho expression was assessed in the brain and spinal cord of different experimental groups at mRNA (qPCR) and protein (ELISA) levels. Also, TAC level was determined in the tissues of different experimental groups. The results showed that Klotho expression in the brain at the onset and peak stages of EAE were significantly lower than that in non-EAE mice. Conversely, Klotho expression in the spinal cord at the onset of EAE was significantly higher than that of non-EAE mice, while Klotho was comparable at the peak stage of EAE and non-EAE mice. The pattern of TAC alteration in the brain and spinal cord of EAE mice was similar to that of Klotho expression. In conclusion, for the first time, this study demonstrated a significant positive correlation between Klotho and TAC changes during the pathogenesis of EAE. It is suggested that Klotho may have neuroprotective activity through the regulation of redox system.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Brain, Experimental Autoimmune Encephalomyelitis (EAE), Klotho, Spinal cord, Total Antioxidant Capacity (TAC)
in
Journal of Molecular Neuroscience
volume
64
issue
4
pages
543 - 550
publisher
Humana Press
external identifiers
  • scopus:85043678429
ISSN
0895-8696
DOI
10.1007/s12031-018-1058-6
language
English
LU publication?
yes
id
f81f7b80-d2d3-4968-8acf-5e9e37644513
date added to LUP
2018-03-27 09:43:27
date last changed
2019-03-19 03:51:48
@article{f81f7b80-d2d3-4968-8acf-5e9e37644513,
  abstract     = {<p>Recently, we reported a positive correlation between Klotho, as an anti-aging protein, and the total antioxidant capacity (TAC) in cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients. However, there is no information about the Klotho and TAC changes within the central nervous system (CNS). Thus, the current study aimed to employ an experimental autoimmune encephalomyelitis (EAE) model in C57BL/6 mice using MOG<sub>35–55</sub> peptide to examine the relationship between Klotho and TAC within the CNS. To this end, the brain and spinal cord were obtained at the onset and peak stages of EAE as well as non-EAE mice (sham/control groups). The Klotho expression was assessed in the brain and spinal cord of different experimental groups at mRNA (qPCR) and protein (ELISA) levels. Also, TAC level was determined in the tissues of different experimental groups. The results showed that Klotho expression in the brain at the onset and peak stages of EAE were significantly lower than that in non-EAE mice. Conversely, Klotho expression in the spinal cord at the onset of EAE was significantly higher than that of non-EAE mice, while Klotho was comparable at the peak stage of EAE and non-EAE mice. The pattern of TAC alteration in the brain and spinal cord of EAE mice was similar to that of Klotho expression. In conclusion, for the first time, this study demonstrated a significant positive correlation between Klotho and TAC changes during the pathogenesis of EAE. It is suggested that Klotho may have neuroprotective activity through the regulation of redox system.</p>},
  author       = {Emami Aleagha, Mohammad Sajad and Harirchian, Mohammad Hossein and Lavasani, Shahram and Javan, Mohammad and Allameh, Abdolamir},
  issn         = {0895-8696},
  keyword      = {Brain,Experimental Autoimmune Encephalomyelitis (EAE),Klotho,Spinal cord,Total Antioxidant Capacity (TAC)},
  language     = {eng},
  month        = {03},
  number       = {4},
  pages        = {543--550},
  publisher    = {Humana Press},
  series       = {Journal of Molecular Neuroscience},
  title        = {Differential Expression of Klotho in the Brain and Spinal Cord is Associated with Total Antioxidant Capacity in Mice with Experimental Autoimmune Encephalomyelitis},
  url          = {http://dx.doi.org/10.1007/s12031-018-1058-6},
  volume       = {64},
  year         = {2018},
}