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Distinct differences in association of MHC class I with endoplasmic reticulum proteins in wild-type, and beta 2-microglobulin- and TAP-deficient cell lines

Paulsson, K M LU ; Wang, P; Anderson, P O LU ; Chen, Shangwu; Pettersson, R F and Li, S LU (2001) In International Immunology 13(8). p.73-1063
Abstract

In this study we have compared the interaction of human MHC class I molecules with IgG heavy chain (HC) binding protein (BiP), calnexin, calreticulin, tapasin and TAP in beta(2)-microglobulin (beta(2)m)- or TAP-deficient cells, as well as in wild-type B-LCL cells. Distinct differences between the association of HC and these endoplasmic reticulum (ER) proteins were found in the three cell lines. In the absence of beta(2)m (Daudi cells), HC associated with both BiP and calnexin. A prominent portion of HC was complexed simultaneously to both chaperones, as indicated by co-precipitation with either anti-calnexin or anti-class I antisera. In the presence of beta(2)m, but absence of TAP (T2 cells), HC could be co-precipitated with calnexin,... (More)

In this study we have compared the interaction of human MHC class I molecules with IgG heavy chain (HC) binding protein (BiP), calnexin, calreticulin, tapasin and TAP in beta(2)-microglobulin (beta(2)m)- or TAP-deficient cells, as well as in wild-type B-LCL cells. Distinct differences between the association of HC and these endoplasmic reticulum (ER) proteins were found in the three cell lines. In the absence of beta(2)m (Daudi cells), HC associated with both BiP and calnexin. A prominent portion of HC was complexed simultaneously to both chaperones, as indicated by co-precipitation with either anti-calnexin or anti-class I antisera. In the presence of beta(2)m, but absence of TAP (T2 cells), HC could be co-precipitated with calnexin, whereas no detectable interaction with BiP could be demonstrated. This suggests that calnexin interacts with HC at a later stage than BiP. In B-LCL cells, HC-beta(2)m associated with calreticulin and tapasin, whereas no interaction with calnexin and BiP was observed. In the absence of beta(2)m, HC were rapidly degraded in the ER, while the ER retained HC were stabilized in the presence of beta(2)m, even in the absence of TAP. The dissociation of class I molecules from TAP in B-LCL cells correlated with the kinetics of appearance of class I molecules on the cell surface, suggesting that TAP retains peptide-free class I molecules in the ER. Taken together, our results suggest the model that BiP and calnexin sequentially control the folding of MHC class I, before MHC class I molecules associate with the loading complex.

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keywords
ATP-Binding Cassette Transporters, Antiporters, Biological Transport, Calcium-Binding Proteins, Calnexin, Calreticulin, Carrier Proteins, Dimerization, Endoplasmic Reticulum, HLA Antigens, Heat-Shock Proteins, Histocompatibility Antigens Class I, Humans, Immunoglobulin G, Immunoglobulins, Membrane Transport Proteins, Molecular Chaperones, Ribonucleoproteins, Tumor Cells, Cultured, beta 2-Microglobulin, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, tapasin
in
International Immunology
volume
13
issue
8
pages
11 pages
publisher
Oxford University Press
external identifiers
  • scopus:0034864986
ISSN
0953-8178
DOI
10.1093/intimm/13.8.1063
language
English
LU publication?
yes
id
f8243a88-6d96-45e3-90d2-d230299a8157
date added to LUP
2016-10-28 09:07:05
date last changed
2018-10-14 04:33:36
@article{f8243a88-6d96-45e3-90d2-d230299a8157,
  abstract     = {<p>In this study we have compared the interaction of human MHC class I molecules with IgG heavy chain (HC) binding protein (BiP), calnexin, calreticulin, tapasin and TAP in beta(2)-microglobulin (beta(2)m)- or TAP-deficient cells, as well as in wild-type B-LCL cells. Distinct differences between the association of HC and these endoplasmic reticulum (ER) proteins were found in the three cell lines. In the absence of beta(2)m (Daudi cells), HC associated with both BiP and calnexin. A prominent portion of HC was complexed simultaneously to both chaperones, as indicated by co-precipitation with either anti-calnexin or anti-class I antisera. In the presence of beta(2)m, but absence of TAP (T2 cells), HC could be co-precipitated with calnexin, whereas no detectable interaction with BiP could be demonstrated. This suggests that calnexin interacts with HC at a later stage than BiP. In B-LCL cells, HC-beta(2)m associated with calreticulin and tapasin, whereas no interaction with calnexin and BiP was observed. In the absence of beta(2)m, HC were rapidly degraded in the ER, while the ER retained HC were stabilized in the presence of beta(2)m, even in the absence of TAP. The dissociation of class I molecules from TAP in B-LCL cells correlated with the kinetics of appearance of class I molecules on the cell surface, suggesting that TAP retains peptide-free class I molecules in the ER. Taken together, our results suggest the model that BiP and calnexin sequentially control the folding of MHC class I, before MHC class I molecules associate with the loading complex.</p>},
  author       = {Paulsson, K M and Wang, P and Anderson, P O and Chen,  Shangwu and Pettersson, R F and Li, S},
  issn         = {0953-8178},
  keyword      = {ATP-Binding Cassette Transporters,Antiporters,Biological Transport,Calcium-Binding Proteins,Calnexin,Calreticulin,Carrier Proteins,Dimerization,Endoplasmic Reticulum,HLA Antigens,Heat-Shock Proteins,Histocompatibility Antigens Class I,Humans,Immunoglobulin G,Immunoglobulins,Membrane Transport Proteins,Molecular Chaperones,Ribonucleoproteins,Tumor Cells, Cultured,beta 2-Microglobulin,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't,tapasin},
  language     = {eng},
  number       = {8},
  pages        = {73--1063},
  publisher    = {Oxford University Press},
  series       = {International Immunology},
  title        = {Distinct differences in association of MHC class I with endoplasmic reticulum proteins in wild-type, and beta 2-microglobulin- and TAP-deficient cell lines},
  url          = {http://dx.doi.org/10.1093/intimm/13.8.1063},
  volume       = {13},
  year         = {2001},
}