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Characterization of protein-protein interactions in recombinant hemoglobin producing escherichia coli cells using molecularly imprinted polymers

Zhang, Ka LU ; Zhou, Tongchang LU ; Ye, Lei LU and Bülow, Leif LU (2017) In Advances in Experimental Medicine and Biology 977. p.367-373
Abstract

The worldwide blood shortage has generated demands for alternatives to transfusible human blood. One such important option is based on recombinant hemoglobin-based oxygen carriers (rHBOCs). Most efforts have been focused on various E. coli based production systems. One of the key challenges in these systems is to devise an efficient and economical protein production strategy involving selection of suitable host cell and Hb variant, growth conditions and media engineering. Hb also influences the heterologous host cell metabolism and therefore the identification of modified protein-protein interactions is critical for optimizing Hb production. In this study, molecularly imprinted polymers (MIPs) directed against Hb were used to identify... (More)

The worldwide blood shortage has generated demands for alternatives to transfusible human blood. One such important option is based on recombinant hemoglobin-based oxygen carriers (rHBOCs). Most efforts have been focused on various E. coli based production systems. One of the key challenges in these systems is to devise an efficient and economical protein production strategy involving selection of suitable host cell and Hb variant, growth conditions and media engineering. Hb also influences the heterologous host cell metabolism and therefore the identification of modified protein-protein interactions is critical for optimizing Hb production. In this study, molecularly imprinted polymers (MIPs) directed against Hb were used to identify the human Hb protein interaction network in E. coli. One E. coli host protein, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), interacted strongly with Hb, especially fetal Hb (HbF).

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Escherichia coli, Glyceraldehyde 3-phosphate dehydrogenase, Molecularly imprinted polymer, Protein-protein interaction, Recombinant hemoglobin
in
Advances in Experimental Medicine and Biology
volume
977
pages
7 pages
publisher
Kluwer
external identifiers
  • scopus:85026364612
ISSN
0065-2598
DOI
10.1007/978-3-319-55231-6_48
language
English
LU publication?
yes
id
f82a9f76-dd79-46a1-9c20-973f4a08ed3f
date added to LUP
2017-08-31 14:37:37
date last changed
2017-09-01 03:00:02
@article{f82a9f76-dd79-46a1-9c20-973f4a08ed3f,
  abstract     = {<p>The worldwide blood shortage has generated demands for alternatives to transfusible human blood. One such important option is based on recombinant hemoglobin-based oxygen carriers (rHBOCs). Most efforts have been focused on various E. coli based production systems. One of the key challenges in these systems is to devise an efficient and economical protein production strategy involving selection of suitable host cell and Hb variant, growth conditions and media engineering. Hb also influences the heterologous host cell metabolism and therefore the identification of modified protein-protein interactions is critical for optimizing Hb production. In this study, molecularly imprinted polymers (MIPs) directed against Hb were used to identify the human Hb protein interaction network in E. coli. One E. coli host protein, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), interacted strongly with Hb, especially fetal Hb (HbF).</p>},
  author       = {Zhang, Ka and Zhou, Tongchang and Ye, Lei and Bülow, Leif},
  issn         = {0065-2598},
  keyword      = {Escherichia coli,Glyceraldehyde 3-phosphate dehydrogenase,Molecularly imprinted polymer,Protein-protein interaction,Recombinant hemoglobin},
  language     = {eng},
  pages        = {367--373},
  publisher    = {Kluwer},
  series       = {Advances in Experimental Medicine and Biology},
  title        = {Characterization of protein-protein interactions in recombinant hemoglobin producing <i>escherichia coli</i> cells using molecularly imprinted polymers},
  url          = {http://dx.doi.org/10.1007/978-3-319-55231-6_48},
  volume       = {977},
  year         = {2017},
}