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Serum neurofilament light protein predicts clinical outcome in traumatic brain injury

Shahim, Pashtun; Gren, Magnus; Liman, Victor; Andreasson, Ulf; Norgren, Niklas; Tegner, Yelverton; Mattsson, Niklas LU ; Andreasen, Niels; Öst, Martin and Zetterberg, Henrik LU , et al. (2016) In Scientific Reports 6.
Abstract

Axonal white matter injury is believed to be a major determinant of adverse outcomes following traumatic brain injury (TBI). We hypothesized that measurement of neurofilament light protein (NF-L), a protein found in long white-matter axons, in blood samples, may serve as a suitable biomarker for neuronal damage in TBI patients. To test our hypotheses, we designed a study in two parts: i) we developed an immunoassay based on Single molecule array technology for quantification of NF-L in blood, and ii) in a proof-of-concept study, we tested our newly developed method on serial serum samples from severe TBI (sTBI) patients (n = 72) and controls (n = 35). We also compared the diagnostic and prognostic utility of NF-L with the established... (More)

Axonal white matter injury is believed to be a major determinant of adverse outcomes following traumatic brain injury (TBI). We hypothesized that measurement of neurofilament light protein (NF-L), a protein found in long white-matter axons, in blood samples, may serve as a suitable biomarker for neuronal damage in TBI patients. To test our hypotheses, we designed a study in two parts: i) we developed an immunoassay based on Single molecule array technology for quantification of NF-L in blood, and ii) in a proof-of-concept study, we tested our newly developed method on serial serum samples from severe TBI (sTBI) patients (n = 72) and controls (n = 35). We also compared the diagnostic and prognostic utility of NF-L with the established blood biomarker S100B. NF-L levels were markedly increased in sTBI patients compared with controls. NF-L at admission yielded an AUC of 0.99 to detect TBI versus controls (AUC 0.96 for S100B), and increased to 1.00 at day 12 (0.65 for S100B). Importantly, initial NF-L levels predicted poor 12-month clinical outcome. In contrast, S100B was not related to outcome. Taken together, our data suggests that measurement of serum NF-L may be useful to assess the severity of neuronal injury following sTBI.

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Scientific Reports
volume
6
publisher
Nature Publishing Group
external identifiers
  • scopus:84994908954
  • wos:000387073100001
ISSN
2045-2322
DOI
10.1038/srep36791
language
English
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yes
id
f8384df6-6a83-4485-80e4-bbf3278bcac6
date added to LUP
2016-12-01 11:07:22
date last changed
2017-10-22 05:22:50
@article{f8384df6-6a83-4485-80e4-bbf3278bcac6,
  abstract     = {<p>Axonal white matter injury is believed to be a major determinant of adverse outcomes following traumatic brain injury (TBI). We hypothesized that measurement of neurofilament light protein (NF-L), a protein found in long white-matter axons, in blood samples, may serve as a suitable biomarker for neuronal damage in TBI patients. To test our hypotheses, we designed a study in two parts: i) we developed an immunoassay based on Single molecule array technology for quantification of NF-L in blood, and ii) in a proof-of-concept study, we tested our newly developed method on serial serum samples from severe TBI (sTBI) patients (n = 72) and controls (n = 35). We also compared the diagnostic and prognostic utility of NF-L with the established blood biomarker S100B. NF-L levels were markedly increased in sTBI patients compared with controls. NF-L at admission yielded an AUC of 0.99 to detect TBI versus controls (AUC 0.96 for S100B), and increased to 1.00 at day 12 (0.65 for S100B). Importantly, initial NF-L levels predicted poor 12-month clinical outcome. In contrast, S100B was not related to outcome. Taken together, our data suggests that measurement of serum NF-L may be useful to assess the severity of neuronal injury following sTBI.</p>},
  articleno    = {36791},
  author       = {Shahim, Pashtun and Gren, Magnus and Liman, Victor and Andreasson, Ulf and Norgren, Niklas and Tegner, Yelverton and Mattsson, Niklas and Andreasen, Niels and Öst, Martin and Zetterberg, Henrik and Nellgård, Bengt and Blennow, Kaj},
  issn         = {2045-2322},
  language     = {eng},
  month        = {11},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Serum neurofilament light protein predicts clinical outcome in traumatic brain injury},
  url          = {http://dx.doi.org/10.1038/srep36791},
  volume       = {6},
  year         = {2016},
}