Using heterokaryons to understand pluripotency and reprogramming
Piccolo, Francesco M. ; Pereira, Carlos F. LU
; Cantone, Irene
; Brown, Karen
; Tsubouchi, Tomomi
; Soza-Ried, Jorge
; Merkenschlager, Matthias
and Fisher, Amanda G.
(2011)
In Philosophical Transactions of the Royal Society B: Biological Sciences
366(1575).
p.2260-2265
- Abstract
Reprogramming differentiated cells towards pluripotency can be achieved by different experimental strategies including the forced expression of specific 'inducers' and nuclear transfer. While these offer unparalleled opportunities to generate stem cells and advance disease modelling, the relatively low levels of successful reprogramming achieved (1-2%) makes a direct analysis of the molecular events associated with productive reprogramming very challenging. The generation of transient heterokaryons between human differentiated cells (such as lymphocytes or fibroblasts) and mouse pluripotent stem cell lines results in a much higher frequency of successful conversion (15% SSEA4 expressing cells) and provides an alternative approach to... (More)
Reprogramming differentiated cells towards pluripotency can be achieved by different experimental strategies including the forced expression of specific 'inducers' and nuclear transfer. While these offer unparalleled opportunities to generate stem cells and advance disease modelling, the relatively low levels of successful reprogramming achieved (1-2%) makes a direct analysis of the molecular events associated with productive reprogramming very challenging. The generation of transient heterokaryons between human differentiated cells (such as lymphocytes or fibroblasts) and mouse pluripotent stem cell lines results in a much higher frequency of successful conversion (15% SSEA4 expressing cells) and provides an alternative approach to study early events during reprogramming. Under these conditions, differentiated nuclei undergo a series of remodelling events before initiating human pluripotent gene expression and silencing differentiation-associated genes. When combined with genetic or RNAi-based approaches and high-throughput screens, heterokaryon studies can provide important new insights into the factors and mechanisms required to reprogramme unipotent cells towards pluripotency.
(Less)
- author
- Piccolo, Francesco M.
; Pereira, Carlos F.
LU
; Cantone, Irene
; Brown, Karen
; Tsubouchi, Tomomi
; Soza-Ried, Jorge
; Merkenschlager, Matthias
and Fisher, Amanda G.
- publishing date
- 2011-07-04
- type
- Contribution to journal
- publication status
- published
- keywords
- Cell fusion, Embryonic stem cell, Heterokaryon, Nuclear organization, Pluripotency, Reprogramming
- in
- Philosophical Transactions of the Royal Society B: Biological Sciences
- volume
- 366
- issue
- 1575
- pages
- 6 pages
- publisher
- Royal Society Publishing
- external identifiers
-
- scopus:79960073520
- pmid:21727131
- ISSN
- 1471-2970
- DOI
- 10.1098/rstb.2011.0004
- language
- English
- LU publication?
- no
- id
- f84d707c-240d-4d10-9f13-c10a1dd06a24
- date added to LUP
- 2017-10-02 17:29:13
- date last changed
- 2024-05-26 23:37:26
@article{f84d707c-240d-4d10-9f13-c10a1dd06a24,
abstract = {{<p>Reprogramming differentiated cells towards pluripotency can be achieved by different experimental strategies including the forced expression of specific 'inducers' and nuclear transfer. While these offer unparalleled opportunities to generate stem cells and advance disease modelling, the relatively low levels of successful reprogramming achieved (1-2%) makes a direct analysis of the molecular events associated with productive reprogramming very challenging. The generation of transient heterokaryons between human differentiated cells (such as lymphocytes or fibroblasts) and mouse pluripotent stem cell lines results in a much higher frequency of successful conversion (15% SSEA4 expressing cells) and provides an alternative approach to study early events during reprogramming. Under these conditions, differentiated nuclei undergo a series of remodelling events before initiating human pluripotent gene expression and silencing differentiation-associated genes. When combined with genetic or RNAi-based approaches and high-throughput screens, heterokaryon studies can provide important new insights into the factors and mechanisms required to reprogramme unipotent cells towards pluripotency.</p>}},
author = {{Piccolo, Francesco M. and Pereira, Carlos F. and Cantone, Irene and Brown, Karen and Tsubouchi, Tomomi and Soza-Ried, Jorge and Merkenschlager, Matthias and Fisher, Amanda G.}},
issn = {{1471-2970}},
keywords = {{Cell fusion; Embryonic stem cell; Heterokaryon; Nuclear organization; Pluripotency; Reprogramming}},
language = {{eng}},
month = {{07}},
number = {{1575}},
pages = {{2260--2265}},
publisher = {{Royal Society Publishing}},
series = {{Philosophical Transactions of the Royal Society B: Biological Sciences}},
title = {{Using heterokaryons to understand pluripotency and reprogramming}},
url = {{http://dx.doi.org/10.1098/rstb.2011.0004}},
doi = {{10.1098/rstb.2011.0004}},
volume = {{366}},
year = {{2011}},
}