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Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease

Puchades, Maja ; Hansson, Sara Folkesson ; Nilsson, Carol L LU ; Andreasen, Niels ; Blennow, Kaj LU and Davidsson, Pia (2003) In Molecular Brain Research 118(1-2). p.6-140
Abstract

By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, beta-trace, retinol-binding protein, kininogen, alpha-1... (More)

By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, beta-trace, retinol-binding protein, kininogen, alpha-1 antitrypsin, cell cycle progression 8 protein, and alpha-1beta glycoprotein were significantly altered in CSF of AD patients compared to controls. Furthermore, we also used liquid-phase IEF, as a prefractionation step, prior to 2-DE for comparison of CSF proteins between individual AD patients and controls. The levels of 37 proteins spots were altered in AD patients. One of the identified proteins, alpha-2-HS glycoprotein, has not previously been linked to AD. Our study shows that several glycoproteins are altered in AD.

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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Aged, Aged, 80 and over, Alzheimer Disease, Apolipoproteins, Biomarkers, Cerebrospinal Fluid Proteins, Electrophoresis, Gel, Two-Dimensional, Female, Glycoproteins, Humans, Male, Proteomics, Reference Values, Journal Article, Research Support, Non-U.S. Gov't
in
Molecular Brain Research
volume
118
issue
1-2
pages
7 pages
publisher
Elsevier
external identifiers
  • pmid:14559363
  • scopus:0141957321
ISSN
0169-328X
DOI
10.1016/j.molbrainres.2003.08.005
language
English
LU publication?
no
id
f85718e5-03c1-4cf3-8f58-911c97561715
date added to LUP
2017-05-16 10:38:20
date last changed
2024-05-12 13:59:19
@article{f85718e5-03c1-4cf3-8f58-911c97561715,
  abstract     = {{<p>By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, beta-trace, retinol-binding protein, kininogen, alpha-1 antitrypsin, cell cycle progression 8 protein, and alpha-1beta glycoprotein were significantly altered in CSF of AD patients compared to controls. Furthermore, we also used liquid-phase IEF, as a prefractionation step, prior to 2-DE for comparison of CSF proteins between individual AD patients and controls. The levels of 37 proteins spots were altered in AD patients. One of the identified proteins, alpha-2-HS glycoprotein, has not previously been linked to AD. Our study shows that several glycoproteins are altered in AD.</p>}},
  author       = {{Puchades, Maja and Hansson, Sara Folkesson and Nilsson, Carol L and Andreasen, Niels and Blennow, Kaj and Davidsson, Pia}},
  issn         = {{0169-328X}},
  keywords     = {{Aged; Aged, 80 and over; Alzheimer Disease; Apolipoproteins; Biomarkers; Cerebrospinal Fluid Proteins; Electrophoresis, Gel, Two-Dimensional; Female; Glycoproteins; Humans; Male; Proteomics; Reference Values; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{1-2}},
  pages        = {{6--140}},
  publisher    = {{Elsevier}},
  series       = {{Molecular Brain Research}},
  title        = {{Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease}},
  url          = {{http://dx.doi.org/10.1016/j.molbrainres.2003.08.005}},
  doi          = {{10.1016/j.molbrainres.2003.08.005}},
  volume       = {{118}},
  year         = {{2003}},
}