Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease
(2003) In Molecular Brain Research 118(1-2). p.6-140- Abstract
By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, beta-trace, retinol-binding protein, kininogen, alpha-1... (More)
By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, beta-trace, retinol-binding protein, kininogen, alpha-1 antitrypsin, cell cycle progression 8 protein, and alpha-1beta glycoprotein were significantly altered in CSF of AD patients compared to controls. Furthermore, we also used liquid-phase IEF, as a prefractionation step, prior to 2-DE for comparison of CSF proteins between individual AD patients and controls. The levels of 37 proteins spots were altered in AD patients. One of the identified proteins, alpha-2-HS glycoprotein, has not previously been linked to AD. Our study shows that several glycoproteins are altered in AD.
(Less)
- author
- Puchades, Maja ; Hansson, Sara Folkesson ; Nilsson, Carol L LU ; Andreasen, Niels ; Blennow, Kaj LU and Davidsson, Pia
- publishing date
- 2003-10-21
- type
- Contribution to journal
- publication status
- published
- keywords
- Aged, Aged, 80 and over, Alzheimer Disease, Apolipoproteins, Biomarkers, Cerebrospinal Fluid Proteins, Electrophoresis, Gel, Two-Dimensional, Female, Glycoproteins, Humans, Male, Proteomics, Reference Values, Journal Article, Research Support, Non-U.S. Gov't
- in
- Molecular Brain Research
- volume
- 118
- issue
- 1-2
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0141957321
- pmid:14559363
- ISSN
- 0169-328X
- DOI
- 10.1016/j.molbrainres.2003.08.005
- language
- English
- LU publication?
- no
- id
- f85718e5-03c1-4cf3-8f58-911c97561715
- date added to LUP
- 2017-05-16 10:38:20
- date last changed
- 2024-05-12 13:59:19
@article{f85718e5-03c1-4cf3-8f58-911c97561715, abstract = {{<p>By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, beta-trace, retinol-binding protein, kininogen, alpha-1 antitrypsin, cell cycle progression 8 protein, and alpha-1beta glycoprotein were significantly altered in CSF of AD patients compared to controls. Furthermore, we also used liquid-phase IEF, as a prefractionation step, prior to 2-DE for comparison of CSF proteins between individual AD patients and controls. The levels of 37 proteins spots were altered in AD patients. One of the identified proteins, alpha-2-HS glycoprotein, has not previously been linked to AD. Our study shows that several glycoproteins are altered in AD.</p>}}, author = {{Puchades, Maja and Hansson, Sara Folkesson and Nilsson, Carol L and Andreasen, Niels and Blennow, Kaj and Davidsson, Pia}}, issn = {{0169-328X}}, keywords = {{Aged; Aged, 80 and over; Alzheimer Disease; Apolipoproteins; Biomarkers; Cerebrospinal Fluid Proteins; Electrophoresis, Gel, Two-Dimensional; Female; Glycoproteins; Humans; Male; Proteomics; Reference Values; Journal Article; Research Support, Non-U.S. Gov't}}, language = {{eng}}, month = {{10}}, number = {{1-2}}, pages = {{6--140}}, publisher = {{Elsevier}}, series = {{Molecular Brain Research}}, title = {{Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease}}, url = {{http://dx.doi.org/10.1016/j.molbrainres.2003.08.005}}, doi = {{10.1016/j.molbrainres.2003.08.005}}, volume = {{118}}, year = {{2003}}, }