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Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease

Puchades, Maja; Hansson, Sara Folkesson; Nilsson, Carol L LU ; Andreasen, Niels; Blennow, Kaj LU and Davidsson, Pia (2003) In Molecular Brain Research 118(1-2). p.6-140
Abstract

By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, beta-trace, retinol-binding protein, kininogen, alpha-1... (More)

By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, beta-trace, retinol-binding protein, kininogen, alpha-1 antitrypsin, cell cycle progression 8 protein, and alpha-1beta glycoprotein were significantly altered in CSF of AD patients compared to controls. Furthermore, we also used liquid-phase IEF, as a prefractionation step, prior to 2-DE for comparison of CSF proteins between individual AD patients and controls. The levels of 37 proteins spots were altered in AD patients. One of the identified proteins, alpha-2-HS glycoprotein, has not previously been linked to AD. Our study shows that several glycoproteins are altered in AD.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Aged, Aged, 80 and over, Alzheimer Disease, Apolipoproteins, Biomarkers, Cerebrospinal Fluid Proteins, Electrophoresis, Gel, Two-Dimensional, Female, Glycoproteins, Humans, Male, Proteomics, Reference Values, Journal Article, Research Support, Non-U.S. Gov't
in
Molecular Brain Research
volume
118
issue
1-2
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:0141957321
ISSN
0169-328X
DOI
10.1016/j.molbrainres.2003.08.005
language
English
LU publication?
no
id
f85718e5-03c1-4cf3-8f58-911c97561715
date added to LUP
2017-05-16 10:38:20
date last changed
2017-09-03 05:22:18
@article{f85718e5-03c1-4cf3-8f58-911c97561715,
  abstract     = {<p>By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer's disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for clinical screening of disease-influenced CSF proteins in AD patients compared to controls. In order to increase the detection of CSF proteins and to improve the separation of protein isoforms in a 2-D gel, micro-narrow range IPG strips were used. The levels of eight proteins and their isoforms, including apolipoprotein A1, apolipoprotein E, apolipoprotein J, beta-trace, retinol-binding protein, kininogen, alpha-1 antitrypsin, cell cycle progression 8 protein, and alpha-1beta glycoprotein were significantly altered in CSF of AD patients compared to controls. Furthermore, we also used liquid-phase IEF, as a prefractionation step, prior to 2-DE for comparison of CSF proteins between individual AD patients and controls. The levels of 37 proteins spots were altered in AD patients. One of the identified proteins, alpha-2-HS glycoprotein, has not previously been linked to AD. Our study shows that several glycoproteins are altered in AD.</p>},
  author       = {Puchades, Maja and Hansson, Sara Folkesson and Nilsson, Carol L and Andreasen, Niels and Blennow, Kaj and Davidsson, Pia},
  issn         = {0169-328X},
  keyword      = {Aged,Aged, 80 and over,Alzheimer Disease,Apolipoproteins,Biomarkers,Cerebrospinal Fluid Proteins,Electrophoresis, Gel, Two-Dimensional,Female,Glycoproteins,Humans,Male,Proteomics,Reference Values,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {10},
  number       = {1-2},
  pages        = {6--140},
  publisher    = {Elsevier},
  series       = {Molecular Brain Research},
  title        = {Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease},
  url          = {http://dx.doi.org/10.1016/j.molbrainres.2003.08.005},
  volume       = {118},
  year         = {2003},
}