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Trophic effect of porcine Sertoli cells on rat and human ventral mesencephalic cells and hNT neurons in vitro

Othberg, A I LU ; Willing, A E; Cameron, D F; Anton, A; Saporta, S; Freeman, T B and Sanberg, P R (1998) In Cell Transplantation 7(2). p.64-157
Abstract

The poor survival of embryonic dopaminergic (DA) neurons transplanted into patients with Parkinson's disease (PD) has encouraged researchers to search for new methods to affect the short- as well as long-term survival of these neurons after transplantation. In several previous rodent studies Sertoli cells increased survival of islet cells and chromaffin cells when cotransplanted in vivo. The aims of this study were to investigate whether porcine Sertoli cells had a positive effect on the survival and maturation of rat and human DA neurons, and whether the Sertoli cells had an effect on differentiation of neurons derived from a human teratocarcinoma cell line (hNT neurons). A significant increase of tyrosine hydroxylase (TH)-positive... (More)

The poor survival of embryonic dopaminergic (DA) neurons transplanted into patients with Parkinson's disease (PD) has encouraged researchers to search for new methods to affect the short- as well as long-term survival of these neurons after transplantation. In several previous rodent studies Sertoli cells increased survival of islet cells and chromaffin cells when cotransplanted in vivo. The aims of this study were to investigate whether porcine Sertoli cells had a positive effect on the survival and maturation of rat and human DA neurons, and whether the Sertoli cells had an effect on differentiation of neurons derived from a human teratocarcinoma cell line (hNT neurons). A significant increase of tyrosine hydroxylase (TH)-positive neurons of both rat and human ventral mesencephalic tissue was found when cocultured with Sertoli cells. Furthermore, there was a significantly increased soma size and neurite outgrowth of neurons in the coculture treated group. The Sertoli cell and hNT coculture also revealed an increased number of TH-positive cells. These results demonstrate that the wide variety of proteins and factors secreted by porcine Sertoli cells benefit the survival and maturation of embryonic DA neurons and suggest that cotransplantation of Sertoli cells and embryonic DA neurons may be useful for a cell transplantation therapy in PD.

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publication status
published
keywords
Animals, Brain Tissue Transplantation, Cell Count, Cell Differentiation, Cell Survival, Coculture Techniques, Fetal Tissue Transplantation, Humans, Male, Mesencephalon, Neurons, Parkinson Disease, Rats, Sertoli Cells, Swine, Tumor Cells, Cultured, Tyrosine 3-Monooxygenase, Journal Article
in
Cell Transplantation
volume
7
issue
2
pages
8 pages
publisher
Cognizant Communication Corporation
external identifiers
  • scopus:0031918809
ISSN
0963-6897
DOI
10.1016/S0963-6897(97)00164-4
language
English
LU publication?
no
id
f86a2cb1-0d77-44ff-8485-cbd96c86118c
date added to LUP
2016-12-11 10:01:58
date last changed
2017-04-09 04:52:40
@article{f86a2cb1-0d77-44ff-8485-cbd96c86118c,
  abstract     = {<p>The poor survival of embryonic dopaminergic (DA) neurons transplanted into patients with Parkinson's disease (PD) has encouraged researchers to search for new methods to affect the short- as well as long-term survival of these neurons after transplantation. In several previous rodent studies Sertoli cells increased survival of islet cells and chromaffin cells when cotransplanted in vivo. The aims of this study were to investigate whether porcine Sertoli cells had a positive effect on the survival and maturation of rat and human DA neurons, and whether the Sertoli cells had an effect on differentiation of neurons derived from a human teratocarcinoma cell line (hNT neurons). A significant increase of tyrosine hydroxylase (TH)-positive neurons of both rat and human ventral mesencephalic tissue was found when cocultured with Sertoli cells. Furthermore, there was a significantly increased soma size and neurite outgrowth of neurons in the coculture treated group. The Sertoli cell and hNT coculture also revealed an increased number of TH-positive cells. These results demonstrate that the wide variety of proteins and factors secreted by porcine Sertoli cells benefit the survival and maturation of embryonic DA neurons and suggest that cotransplantation of Sertoli cells and embryonic DA neurons may be useful for a cell transplantation therapy in PD.</p>},
  author       = {Othberg, A I and Willing, A E and Cameron, D F and Anton, A and Saporta, S and Freeman, T B and Sanberg, P R},
  issn         = {0963-6897},
  keyword      = {Animals,Brain Tissue Transplantation,Cell Count,Cell Differentiation,Cell Survival,Coculture Techniques,Fetal Tissue Transplantation,Humans,Male,Mesencephalon,Neurons,Parkinson Disease,Rats,Sertoli Cells,Swine,Tumor Cells, Cultured,Tyrosine 3-Monooxygenase,Journal Article},
  language     = {eng},
  month        = {05},
  number       = {2},
  pages        = {64--157},
  publisher    = {Cognizant Communication Corporation},
  series       = {Cell Transplantation},
  title        = {Trophic effect of porcine Sertoli cells on rat and human ventral mesencephalic cells and hNT neurons in vitro},
  url          = {http://dx.doi.org/10.1016/S0963-6897(97)00164-4},
  volume       = {7},
  year         = {1998},
}