Structural proteomics, electron cryo-microscopy and structural modeling approaches in bacteria-human protein interactions
Chowdhury, Sounak LU ; Happonen, Lotta LU ; Khakzad, Hamed ; Malmström, Lars LU and Malmström, Johan LU
(2020)
In Medical microbiology and immunology
209(3).
p.265-275
- Abstract
A central challenge in infection medicine is to determine the structure and function of host-pathogen protein-protein interactions to understand how these interactions facilitate bacterial adhesion, dissemination and survival. In this review, we focus on proteomics, electron cryo-microscopy and structural modeling to showcase instances where affinity-purification (AP) and cross-linking (XL) mass spectrometry (MS) has advanced our understanding of host-pathogen interactions. We highlight cases where XL-MS in combination with structural modeling has provided insight into the quaternary structure of interspecies protein complexes. We further exemplify how electron cryo-tomography has been used to visualize bacterial-human interactions... (More)
A central challenge in infection medicine is to determine the structure and function of host-pathogen protein-protein interactions to understand how these interactions facilitate bacterial adhesion, dissemination and survival. In this review, we focus on proteomics, electron cryo-microscopy and structural modeling to showcase instances where affinity-purification (AP) and cross-linking (XL) mass spectrometry (MS) has advanced our understanding of host-pathogen interactions. We highlight cases where XL-MS in combination with structural modeling has provided insight into the quaternary structure of interspecies protein complexes. We further exemplify how electron cryo-tomography has been used to visualize bacterial-human interactions during attachment and infection. Lastly, we discuss how AP-MS, XL-MS and electron cryo-microscopy and -tomography together with structural modeling approaches can be used in future studies to broaden our knowledge regarding the function, dynamics and evolution of such interactions. This knowledge will be of relevance for future drug and vaccine development programs.
(Less)
- author
- Chowdhury, Sounak
LU
; Happonen, Lotta
LU
; Khakzad, Hamed
; Malmström, Lars
LU
and Malmström, Johan
LU
- organization
- publishing date
- 2020-06
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Medical microbiology and immunology
- volume
- 209
- issue
- 3
- pages
- 11 pages
- publisher
- Springer
- external identifiers
-
- pmid:32072248
- scopus:85079779120
- ISSN
- 0300-8584
- DOI
- 10.1007/s00430-020-00663-5
- language
- English
- LU publication?
- yes
- id
- f87cb61a-f70a-430f-86a2-3317b8f65ae7
- date added to LUP
- 2020-02-22 21:36:57
- date last changed
- 2024-05-29 09:23:41
@article{f87cb61a-f70a-430f-86a2-3317b8f65ae7,
abstract = {{<p>A central challenge in infection medicine is to determine the structure and function of host-pathogen protein-protein interactions to understand how these interactions facilitate bacterial adhesion, dissemination and survival. In this review, we focus on proteomics, electron cryo-microscopy and structural modeling to showcase instances where affinity-purification (AP) and cross-linking (XL) mass spectrometry (MS) has advanced our understanding of host-pathogen interactions. We highlight cases where XL-MS in combination with structural modeling has provided insight into the quaternary structure of interspecies protein complexes. We further exemplify how electron cryo-tomography has been used to visualize bacterial-human interactions during attachment and infection. Lastly, we discuss how AP-MS, XL-MS and electron cryo-microscopy and -tomography together with structural modeling approaches can be used in future studies to broaden our knowledge regarding the function, dynamics and evolution of such interactions. This knowledge will be of relevance for future drug and vaccine development programs.</p>}},
author = {{Chowdhury, Sounak and Happonen, Lotta and Khakzad, Hamed and Malmström, Lars and Malmström, Johan}},
issn = {{0300-8584}},
language = {{eng}},
number = {{3}},
pages = {{265--275}},
publisher = {{Springer}},
series = {{Medical microbiology and immunology}},
title = {{Structural proteomics, electron cryo-microscopy and structural modeling approaches in bacteria-human protein interactions}},
url = {{http://dx.doi.org/10.1007/s00430-020-00663-5}},
doi = {{10.1007/s00430-020-00663-5}},
volume = {{209}},
year = {{2020}},
}