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Bacillus subtilis citM, the structural gene for dihydrolipoamide transsuccinylase: cloning and expression in Escherichia coli

Carlsson, Peter and Hederstedt, Lars LU (1987) In Gene 61(2). p.217-224
Abstract
The 2-oxoglutarate dehydrogenase multienzyme complex is composed of three different subenzymes: 2-oxoglutarate dehydrogenase (E1o), dihydrolipoamide transsuccinylase (E2o), and dihydrolipoamide dehydrogenase (E3). Bacillus subtilis E1o and E2o are encoded by the citK and citM genes, respectively. A 3.4-kb BamHI DNA fragment containing citK and citM markers was isolated from a library of B. subtilis DNA in Escherichia coli. Functional E2o was expressed from the cloned DNA both in B. subtilis and E. coli. E2o had an apparent Mr of 60000 when expressed in E. coli. The B. subtilis E2o component complemented an E. coli E2o-defective mutant in vivo and in vitro. It is concluded that functional B. subtilis E2o can be produced in E. coli and can... (More)
The 2-oxoglutarate dehydrogenase multienzyme complex is composed of three different subenzymes: 2-oxoglutarate dehydrogenase (E1o), dihydrolipoamide transsuccinylase (E2o), and dihydrolipoamide dehydrogenase (E3). Bacillus subtilis E1o and E2o are encoded by the citK and citM genes, respectively. A 3.4-kb BamHI DNA fragment containing citK and citM markers was isolated from a library of B. subtilis DNA in Escherichia coli. Functional E2o was expressed from the cloned DNA both in B. subtilis and E. coli. E2o had an apparent Mr of 60000 when expressed in E. coli. The B. subtilis E2o component complemented an E. coli E2o-defective mutant in vivo and in vitro. It is concluded that functional B. subtilis E2o can be produced in E. coli and can interact with E. coli and E1o and E3 to form an active chimeric enzyme complex. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Recombinant DNA, 2-oxoglutarate dehydrogenase complex, complementation, lipoyl containing enzyme, multienzyme complexes, citK
in
Gene
volume
61
issue
2
pages
217 - 224
publisher
Elsevier
ISSN
1879-0038
DOI
10.1016/0378-1119(87)90116-8
language
English
LU publication?
yes
id
f8b07abe-92c8-4537-b245-9a80b7fe538f
date added to LUP
2017-07-18 10:57:54
date last changed
2017-09-26 13:51:00
@article{f8b07abe-92c8-4537-b245-9a80b7fe538f,
  abstract     = {The 2-oxoglutarate dehydrogenase multienzyme complex is composed of three different subenzymes: 2-oxoglutarate dehydrogenase (E1o), dihydrolipoamide transsuccinylase (E2o), and dihydrolipoamide dehydrogenase (E3). Bacillus subtilis E1o and E2o are encoded by the citK and citM genes, respectively. A 3.4-kb BamHI DNA fragment containing citK and citM markers was isolated from a library of B. subtilis DNA in Escherichia coli. Functional E2o was expressed from the cloned DNA both in B. subtilis and E. coli. E2o had an apparent Mr of 60000 when expressed in E. coli. The B. subtilis E2o component complemented an E. coli E2o-defective mutant in vivo and in vitro. It is concluded that functional B. subtilis E2o can be produced in E. coli and can interact with E. coli and E1o and E3 to form an active chimeric enzyme complex.},
  author       = {Carlsson, Peter and Hederstedt, Lars},
  issn         = {1879-0038},
  keyword      = {Recombinant DNA,2-oxoglutarate dehydrogenase complex,complementation,lipoyl containing enzyme,multienzyme complexes,citK},
  language     = {eng},
  number       = {2},
  pages        = {217--224},
  publisher    = {Elsevier},
  series       = {Gene},
  title        = {<em>Bacillus subtilis citM</em>, the structural gene for dihydrolipoamide transsuccinylase: cloning and expression in <em>Escherichia coli</em>},
  url          = {http://dx.doi.org/10.1016/0378-1119(87)90116-8},
  volume       = {61},
  year         = {1987},
}