Bacillus subtilis citM, the structural gene for dihydrolipoamide transsuccinylase: cloning and expression in Escherichia coli
(1987) In Gene 61(2). p.217-224- Abstract
- The 2-oxoglutarate dehydrogenase multienzyme complex is composed of three different subenzymes: 2-oxoglutarate dehydrogenase (E1o), dihydrolipoamide transsuccinylase (E2o), and dihydrolipoamide dehydrogenase (E3). Bacillus subtilis E1o and E2o are encoded by the citK and citM genes, respectively. A 3.4-kb BamHI DNA fragment containing citK and citM markers was isolated from a library of B. subtilis DNA in Escherichia coli. Functional E2o was expressed from the cloned DNA both in B. subtilis and E. coli. E2o had an apparent Mr of 60000 when expressed in E. coli. The B. subtilis E2o component complemented an E. coli E2o-defective mutant in vivo and in vitro. It is concluded that functional B. subtilis E2o can be produced in E. coli and can... (More)
- The 2-oxoglutarate dehydrogenase multienzyme complex is composed of three different subenzymes: 2-oxoglutarate dehydrogenase (E1o), dihydrolipoamide transsuccinylase (E2o), and dihydrolipoamide dehydrogenase (E3). Bacillus subtilis E1o and E2o are encoded by the citK and citM genes, respectively. A 3.4-kb BamHI DNA fragment containing citK and citM markers was isolated from a library of B. subtilis DNA in Escherichia coli. Functional E2o was expressed from the cloned DNA both in B. subtilis and E. coli. E2o had an apparent Mr of 60000 when expressed in E. coli. The B. subtilis E2o component complemented an E. coli E2o-defective mutant in vivo and in vitro. It is concluded that functional B. subtilis E2o can be produced in E. coli and can interact with E. coli and E1o and E3 to form an active chimeric enzyme complex. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/f8b07abe-92c8-4537-b245-9a80b7fe538f
- author
- Carlsson, Peter and Hederstedt, Lars LU
- organization
- publishing date
- 1987
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Recombinant DNA, 2-oxoglutarate dehydrogenase complex, complementation, lipoyl containing enzyme, multienzyme complexes, citK
- in
- Gene
- volume
- 61
- issue
- 2
- pages
- 217 - 224
- publisher
- Elsevier
- external identifiers
-
- scopus:0023549944
- ISSN
- 1879-0038
- DOI
- 10.1016/0378-1119(87)90116-8
- language
- English
- LU publication?
- yes
- id
- f8b07abe-92c8-4537-b245-9a80b7fe538f
- date added to LUP
- 2017-07-18 10:57:54
- date last changed
- 2021-01-03 09:17:31
@article{f8b07abe-92c8-4537-b245-9a80b7fe538f,
abstract = {{The 2-oxoglutarate dehydrogenase multienzyme complex is composed of three different subenzymes: 2-oxoglutarate dehydrogenase (E1o), dihydrolipoamide transsuccinylase (E2o), and dihydrolipoamide dehydrogenase (E3). Bacillus subtilis E1o and E2o are encoded by the citK and citM genes, respectively. A 3.4-kb BamHI DNA fragment containing citK and citM markers was isolated from a library of B. subtilis DNA in Escherichia coli. Functional E2o was expressed from the cloned DNA both in B. subtilis and E. coli. E2o had an apparent Mr of 60000 when expressed in E. coli. The B. subtilis E2o component complemented an E. coli E2o-defective mutant in vivo and in vitro. It is concluded that functional B. subtilis E2o can be produced in E. coli and can interact with E. coli and E1o and E3 to form an active chimeric enzyme complex.}},
author = {{Carlsson, Peter and Hederstedt, Lars}},
issn = {{1879-0038}},
keywords = {{Recombinant DNA; 2-oxoglutarate dehydrogenase complex; complementation; lipoyl containing enzyme; multienzyme complexes; citK}},
language = {{eng}},
number = {{2}},
pages = {{217--224}},
publisher = {{Elsevier}},
series = {{Gene}},
title = {{<em>Bacillus subtilis citM</em>, the structural gene for dihydrolipoamide transsuccinylase: cloning and expression in <em>Escherichia coli</em>}},
url = {{http://dx.doi.org/10.1016/0378-1119(87)90116-8}},
doi = {{10.1016/0378-1119(87)90116-8}},
volume = {{61}},
year = {{1987}},
}