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Gel chromatography column scanning for the analysis of 99mTc-labelled compounds

Persson, Bertil R.R. LU and Darte, Lennart LU (1974) In Journal of Chromatography A 101(2). p.315-326
Abstract

The gel chromatography column scanning (GCS) method has been studied, special attention being paid to its suitability for the analysis of 99mTc-labelled compounds and radio-pharmaceuticals. The sample to be analyzed was applied at the top of a column filled with Sephadex G gel. Elution was carried out with 0.9% sodium chloride solution with such a volume that all the radioactivity was retained in the column. The column was then sealed and scanned with a 1 mm slit-collimated NaI(Tl) crystal. The GCS method is discussed and different factors influencing the scanning profile are considered. The relationship between the position of the activity peak in the scanning profile and the molecular weight of the sample is given for... (More)

The gel chromatography column scanning (GCS) method has been studied, special attention being paid to its suitability for the analysis of 99mTc-labelled compounds and radio-pharmaceuticals. The sample to be analyzed was applied at the top of a column filled with Sephadex G gel. Elution was carried out with 0.9% sodium chloride solution with such a volume that all the radioactivity was retained in the column. The column was then sealed and scanned with a 1 mm slit-collimated NaI(Tl) crystal. The GCS method is discussed and different factors influencing the scanning profile are considered. The relationship between the position of the activity peak in the scanning profile and the molecular weight of the sample is given for Sephadex G-25 Medium gel.

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publication status
published
in
Journal of Chromatography A
volume
101
issue
2
pages
12 pages
publisher
Elsevier
external identifiers
  • pmid:4443398
  • scopus:0016396531
ISSN
0021-9673
DOI
10.1016/S0021-9673(00)82849-6
language
English
LU publication?
yes
id
f8f5e31f-d98c-4b96-af7b-6ce6e145f45d
date added to LUP
2020-08-30 21:07:30
date last changed
2020-09-01 16:04:09
@article{f8f5e31f-d98c-4b96-af7b-6ce6e145f45d,
  abstract     = {<p>The gel chromatography column scanning (GCS) method has been studied, special attention being paid to its suitability for the analysis of <sup>99m</sup>Tc-labelled compounds and radio-pharmaceuticals. The sample to be analyzed was applied at the top of a column filled with Sephadex G gel. Elution was carried out with 0.9% sodium chloride solution with such a volume that all the radioactivity was retained in the column. The column was then sealed and scanned with a 1 mm slit-collimated NaI(Tl) crystal. The GCS method is discussed and different factors influencing the scanning profile are considered. The relationship between the position of the activity peak in the scanning profile and the molecular weight of the sample is given for Sephadex G-25 Medium gel.</p>},
  author       = {Persson, Bertil R.R. and Darte, Lennart},
  issn         = {0021-9673},
  language     = {eng},
  month        = {12},
  number       = {2},
  pages        = {315--326},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Gel chromatography column scanning for the analysis of <sup>99m</sup>Tc-labelled compounds},
  url          = {http://dx.doi.org/10.1016/S0021-9673(00)82849-6},
  doi          = {10.1016/S0021-9673(00)82849-6},
  volume       = {101},
  year         = {1974},
}