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Aqueous two-phase systems : A general overview

Hatti-Kaul, Rajni LU (2001) In Molecular Biotechnology 19(3). p.269-277
Abstract
Biphasic systems formed by mixing of two polymers or a polymer and a salt in water can be used for separation of cells, membranes, viruses, proteins, nucleic acids, and other biomolecules. The partitioning between the two phases is dependent on the surface properties and conformation of the materials, and also on the composition of the two-phase system. The mechanism of partitioning is, however, complex and not easily predicted. Aqueous two-phase systems (ATPS) have proven to be a useful tool for analysis of biomolecular and cellular surfaces and their interactions, fractionation of cell populations, product recovery in biotechnology, and so forth. Potential for environmental remediation has also been suggested. Because ATPS are easily... (More)
Biphasic systems formed by mixing of two polymers or a polymer and a salt in water can be used for separation of cells, membranes, viruses, proteins, nucleic acids, and other biomolecules. The partitioning between the two phases is dependent on the surface properties and conformation of the materials, and also on the composition of the two-phase system. The mechanism of partitioning is, however, complex and not easily predicted. Aqueous two-phase systems (ATPS) have proven to be a useful tool for analysis of biomolecular and cellular surfaces and their interactions, fractionation of cell populations, product recovery in biotechnology, and so forth. Potential for environmental remediation has also been suggested. Because ATPS are easily scalable and are also able to hold high biomass load in comparison with other separation techniques, the application that has attracted most interest so far has been the large-scale recovery of proteins from crude feedstocks. As chemicals constitute the major cost factor for large-scale systems, use of easily recyclable phase components and the phase systems generated by a single-phase chemical in water are being studied. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aqueous two-phase system, partition, bioseparation, extraction
in
Molecular Biotechnology
volume
19
issue
3
pages
9 pages
publisher
Humana Press
ISSN
1559-0305
DOI
10.1385/MB:19:3:269
language
English
LU publication?
yes
id
f928895b-46c5-4bc8-9b26-1e57ae3bf30e
date added to LUP
2019-06-29 23:27:43
date last changed
2020-12-27 04:02:04
@article{f928895b-46c5-4bc8-9b26-1e57ae3bf30e,
  abstract     = {Biphasic systems formed by mixing of two polymers or a polymer and a salt in water can be used for separation of cells, membranes, viruses, proteins, nucleic acids, and other biomolecules. The partitioning between the two phases is dependent on the surface properties and conformation of the materials, and also on the composition of the two-phase system. The mechanism of partitioning is, however, complex and not easily predicted. Aqueous two-phase systems (ATPS) have proven to be a useful tool for analysis of biomolecular and cellular surfaces and their interactions, fractionation of cell populations, product recovery in biotechnology, and so forth. Potential for environmental remediation has also been suggested. Because ATPS are easily scalable and are also able to hold high biomass load in comparison with other separation techniques, the application that has attracted most interest so far has been the large-scale recovery of proteins from crude feedstocks. As chemicals constitute the major cost factor for large-scale systems, use of easily recyclable phase components and the phase systems generated by a single-phase chemical in water are being studied.},
  author       = {Hatti-Kaul, Rajni},
  issn         = {1559-0305},
  language     = {eng},
  number       = {3},
  pages        = {269--277},
  publisher    = {Humana Press},
  series       = {Molecular Biotechnology},
  title        = {Aqueous two-phase systems : A general overview},
  url          = {http://dx.doi.org/10.1385/MB:19:3:269},
  doi          = {10.1385/MB:19:3:269},
  volume       = {19},
  year         = {2001},
}