Pressure-Related Effects on Homeostatic Müller Cell Proteins in the Adult Porcine in Vitro Retina
Svare, Frida LU
and Ghosh, Fredrik
LU
(2024)
In Current Eye Research
49(3).
p.303-313
- Abstract
Purpose: To explore early pressure-related effects on Müller cell homeostatic proteins in the in vitro adult porcine retina. Methods: Retinal explants were subjected to 0-, 10-, 30-, or 60-mmHg of pressure for 24 or 48 h in culture. Retinal explants fixed immediately after enucleation were used as controls. Müller cell proteins were evaluated by GFAP, GS, CRALBP, and bFGF immunohistochemistry. Results: GFAP-labeling revealed no differences in fluorescence intensity after 24 or 48 h in any of the pressure groups compared with control retinas. However, a higher intensity was found in the 30- and 60-mmHg groups compared with 0-mmHg counterparts after 24 and 48 h. A higher intensity in GS-labeled sections was found in the 10-and 60-mmHg... (More)
Purpose: To explore early pressure-related effects on Müller cell homeostatic proteins in the in vitro adult porcine retina. Methods: Retinal explants were subjected to 0-, 10-, 30-, or 60-mmHg of pressure for 24 or 48 h in culture. Retinal explants fixed immediately after enucleation were used as controls. Müller cell proteins were evaluated by GFAP, GS, CRALBP, and bFGF immunohistochemistry. Results: GFAP-labeling revealed no differences in fluorescence intensity after 24 or 48 h in any of the pressure groups compared with control retinas. However, a higher intensity was found in the 30- and 60-mmHg groups compared with 0-mmHg counterparts after 24 and 48 h. A higher intensity in GS-labeled sections was found in the 10-and 60-mmHg groups compared with controls and remaining pressure groups after 48 h. Compared with control retinas, CRALBP labeling revealed a higher intensity in the 60-mmHg group after 24 h and in the 10-, 30-, and 60-mmHg groups after 48 h. After 24 and 48 h, a lower intensity was found in bFGF-labeled cells in the 0-, 10-, and 30-mmHg groups compared with controls, while no difference was seen for the 60-mmHg group. Conclusions: Müller cells in the cultured porcine adult retina respond early to pressure by altering the expression of GFAP as well as the homeostatic proteins GS, CRALBP, and bFGF.
(Less)
- author
- Svare, Frida
LU
and Ghosh, Fredrik
LU
- organization
- publishing date
- 2024
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- GFAP, Müller cells, Pressure-related effects, retinal explants
- in
- Current Eye Research
- volume
- 49
- issue
- 3
- pages
- 11 pages
- publisher
- Taylor & Francis
- external identifiers
-
- pmid:38078662
- scopus:85185832594
- ISSN
- 0271-3683
- DOI
- 10.1080/02713683.2023.2286932
- language
- English
- LU publication?
- yes
- id
- f979951c-5455-4148-872a-162afb398f28
- date added to LUP
- 2024-03-20 11:30:55
- date last changed
- 2024-04-17 10:58:32
@article{f979951c-5455-4148-872a-162afb398f28,
abstract = {{<p>Purpose: To explore early pressure-related effects on Müller cell homeostatic proteins in the in vitro adult porcine retina. Methods: Retinal explants were subjected to 0-, 10-, 30-, or 60-mmHg of pressure for 24 or 48 h in culture. Retinal explants fixed immediately after enucleation were used as controls. Müller cell proteins were evaluated by GFAP, GS, CRALBP, and bFGF immunohistochemistry. Results: GFAP-labeling revealed no differences in fluorescence intensity after 24 or 48 h in any of the pressure groups compared with control retinas. However, a higher intensity was found in the 30- and 60-mmHg groups compared with 0-mmHg counterparts after 24 and 48 h. A higher intensity in GS-labeled sections was found in the 10-and 60-mmHg groups compared with controls and remaining pressure groups after 48 h. Compared with control retinas, CRALBP labeling revealed a higher intensity in the 60-mmHg group after 24 h and in the 10-, 30-, and 60-mmHg groups after 48 h. After 24 and 48 h, a lower intensity was found in bFGF-labeled cells in the 0-, 10-, and 30-mmHg groups compared with controls, while no difference was seen for the 60-mmHg group. Conclusions: Müller cells in the cultured porcine adult retina respond early to pressure by altering the expression of GFAP as well as the homeostatic proteins GS, CRALBP, and bFGF.</p>}},
author = {{Svare, Frida and Ghosh, Fredrik}},
issn = {{0271-3683}},
keywords = {{GFAP; Müller cells; Pressure-related effects; retinal explants}},
language = {{eng}},
number = {{3}},
pages = {{303--313}},
publisher = {{Taylor & Francis}},
series = {{Current Eye Research}},
title = {{Pressure-Related Effects on Homeostatic Müller Cell Proteins in the Adult Porcine in Vitro Retina}},
url = {{http://dx.doi.org/10.1080/02713683.2023.2286932}},
doi = {{10.1080/02713683.2023.2286932}},
volume = {{49}},
year = {{2024}},
}