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Polyphosphate formation by Acinetobacter johnsonii strain 210A: effect of cellular energy status and phosphate-specific transport system

van Niel, Ed LU ; de Best, JH; Kets, EPW; Bonting, CFC and Kortstee, GJJ (1999) In Applied Microbiology and Biotechnology 51(5). p.639-646
Abstract
In acetate-limited chemostat cultures of Acinetobacter johnsonii 210A at a dilution rate of 0.1 h−1 the polyphosphate content of the cells increased from 13% to 24% of the biomass dry weight by glucose (100 mM), which was only oxidized to gluconic acid. At this dilution rate, only about 17% of the energy from glucose oxidation was calculated to be used for polyphosphate synthesis, the remaining 83% being used for biomass formation. Suspensions of non-growing, phosphate-deficient cells had a six- to tenfold increased uptake rate of phosphate and accumulated polyphosphate aerobically up to 53% of the biomass dry weight when supplied with only orthophosphate and Mg2+. The initial polyphosphate synthesis rate was 98 ± 17 nmol phosphate min−1... (More)
In acetate-limited chemostat cultures of Acinetobacter johnsonii 210A at a dilution rate of 0.1 h−1 the polyphosphate content of the cells increased from 13% to 24% of the biomass dry weight by glucose (100 mM), which was only oxidized to gluconic acid. At this dilution rate, only about 17% of the energy from glucose oxidation was calculated to be used for polyphosphate synthesis, the remaining 83% being used for biomass formation. Suspensions of non-growing, phosphate-deficient cells had a six- to tenfold increased uptake rate of phosphate and accumulated polyphosphate aerobically up to 53% of the biomass dry weight when supplied with only orthophosphate and Mg2+. The initial polyphosphate synthesis rate was 98 ± 17 nmol phosphate min−1 mg protein−1. Intracellular poly-β-hydroxybutyrate and lipids served as energy sources for the active uptake of phosphate and its subsequent sequestration to polyphosphate. The H+-ATPase inhibitor N,N′-dicyclohexylcarbodiimide caused low ATP levels and a severe inhibition of polyphosphate formation, suggesting the involvement of polyphosphate kinase in polyphosphate synthesis. It is concluded that, in A. johnsonii 210A, (i) polyphosphate is accumulated as the energy supply is in excess of that required for biosynthesis, (ii) not only intracellular poly-β-hydroxybutyrate but also neutral lipids can serve as an energy source for polyphosphate-kinase-mediated polyphosphate formation, (iii) phosphate-deficient cells may accumulate as much polyphosphate as activated sludges and recombinants of Escherichia coli designed for polyphosphate accumulation. (Less)
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publication status
published
keywords
Acinetobacter johnsonii, polyphosphate, continuous culture
in
Applied Microbiology and Biotechnology
volume
51
issue
5
pages
8 pages
publisher
Springer
ISSN
1432-0614
language
English
LU publication?
no
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f993963a-3e2c-4508-be5d-e248eb1f00de
date added to LUP
2016-09-11 21:16:16
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2017-06-16 13:50:39
@article{f993963a-3e2c-4508-be5d-e248eb1f00de,
  abstract     = {In acetate-limited chemostat cultures of Acinetobacter johnsonii 210A at a dilution rate of 0.1 h−1 the polyphosphate content of the cells increased from 13% to 24% of the biomass dry weight by glucose (100 mM), which was only oxidized to gluconic acid. At this dilution rate, only about 17% of the energy from glucose oxidation was calculated to be used for polyphosphate synthesis, the remaining 83% being used for biomass formation. Suspensions of non-growing, phosphate-deficient cells had a six- to tenfold increased uptake rate of phosphate and accumulated polyphosphate aerobically up to 53% of the biomass dry weight when supplied with only orthophosphate and Mg2+. The initial polyphosphate synthesis rate was 98 ± 17 nmol phosphate min−1 mg protein−1. Intracellular poly-β-hydroxybutyrate and lipids served as energy sources for the active uptake of phosphate and its subsequent sequestration to polyphosphate. The H+-ATPase inhibitor N,N′-dicyclohexylcarbodiimide caused low ATP levels and a severe inhibition of polyphosphate formation, suggesting the involvement of polyphosphate kinase in polyphosphate synthesis. It is concluded that, in A. johnsonii 210A, (i) polyphosphate is accumulated as the energy supply is in excess of that required for biosynthesis, (ii) not only intracellular poly-β-hydroxybutyrate but also neutral lipids can serve as an energy source for polyphosphate-kinase-mediated polyphosphate formation, (iii) phosphate-deficient cells may accumulate as much polyphosphate as activated sludges and recombinants of Escherichia coli designed for polyphosphate accumulation.},
  author       = {van Niel, Ed and de Best, JH and Kets, EPW and Bonting, CFC and Kortstee, GJJ},
  issn         = {1432-0614},
  keyword      = {Acinetobacter johnsonii,polyphosphate,continuous culture},
  language     = {eng},
  number       = {5},
  pages        = {639--646},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {Polyphosphate formation by Acinetobacter johnsonii strain 210A: effect of cellular energy status and phosphate-specific transport system},
  volume       = {51},
  year         = {1999},
}