Effects of total parenteral nutrition on rat enteric nervous system, intestinal morphology, and motility.
(2005) In Journal of Surgical Research 124(2). p.187-193- Abstract
- Total parenteral nutrition (TPN) is often crucial for patients not being able to feed enterally or having intestinal absorptive deficits. Enteral nutrition is, however, frequently regarded vital for maintaining functional and structural intestinal integrity. The aim of this study was to investigate possible effects of TPN on rat distal small intestine compared to enterally fed identically housed controls, regarding the enteric nervous system (ENS), motility in vitro, and morphology. This study shows that motor responses evoked by electrical stimulation or exposure to vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptide-27 (PACAP-27), and nitric oxide (NO) donor were unchanged. By using immunohistochemistry,... (More)
- Total parenteral nutrition (TPN) is often crucial for patients not being able to feed enterally or having intestinal absorptive deficits. Enteral nutrition is, however, frequently regarded vital for maintaining functional and structural intestinal integrity. The aim of this study was to investigate possible effects of TPN on rat distal small intestine compared to enterally fed identically housed controls, regarding the enteric nervous system (ENS), motility in vitro, and morphology. This study shows that motor responses evoked by electrical stimulation or exposure to vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptide-27 (PACAP-27), and nitric oxide (NO) donor were unchanged. By using immunohistochemistry, the numbers of submucous (P < 0.05) and myenteric (P < 0.05) nerve cells were found to increase, expressed as numbers per unit length. The percentage of neurons expressing VIP, PACAP-27, NO-synthase, and galanin remained unchanged, however. By in situ hybridization the number of submucous neurons expressing neuropeptide Y-mRNA was found to decrease (P < 0.05); the other populations were unaltered. Morphometry revealed an increased submucosal thickness (P < 0.05), while intestinal circumference markedly decreased (P < 0.0001) in TPN-treated rats. In conclusion, TPN treatment resulted in reduced intestinal circumference leading to condensation of enteric neurons. No marked changes in neurotransmitter expression of the enteric neurons or in motor activity were noted. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/136281
- author
- Ekelund, Mikael LU ; Ekelund, Mats LU ; Qader, Saleem LU ; Hallén, Magnus LU and Ekblad, Eva LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- plasticity, motility, morphology, ENS, intestine, rat, TPN
- in
- Journal of Surgical Research
- volume
- 124
- issue
- 2
- pages
- 187 - 193
- publisher
- Elsevier
- external identifiers
-
- pmid:15820247
- wos:000228275800006
- scopus:16244385635
- pmid:15820247
- ISSN
- 1095-8673
- DOI
- 10.1016/j.jss.2004.10.008
- language
- English
- LU publication?
- yes
- id
- f9aa1556-7df9-4bf9-8496-be1feb0ea402 (old id 136281)
- alternative location
- http://www.sciencedirect.com/science/article/pii/S0022480404006365
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15820247&dopt=Abstract
- date added to LUP
- 2016-04-01 15:49:03
- date last changed
- 2022-03-07 01:35:00
@article{f9aa1556-7df9-4bf9-8496-be1feb0ea402, abstract = {{Total parenteral nutrition (TPN) is often crucial for patients not being able to feed enterally or having intestinal absorptive deficits. Enteral nutrition is, however, frequently regarded vital for maintaining functional and structural intestinal integrity. The aim of this study was to investigate possible effects of TPN on rat distal small intestine compared to enterally fed identically housed controls, regarding the enteric nervous system (ENS), motility in vitro, and morphology. This study shows that motor responses evoked by electrical stimulation or exposure to vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptide-27 (PACAP-27), and nitric oxide (NO) donor were unchanged. By using immunohistochemistry, the numbers of submucous (P < 0.05) and myenteric (P < 0.05) nerve cells were found to increase, expressed as numbers per unit length. The percentage of neurons expressing VIP, PACAP-27, NO-synthase, and galanin remained unchanged, however. By in situ hybridization the number of submucous neurons expressing neuropeptide Y-mRNA was found to decrease (P < 0.05); the other populations were unaltered. Morphometry revealed an increased submucosal thickness (P < 0.05), while intestinal circumference markedly decreased (P < 0.0001) in TPN-treated rats. In conclusion, TPN treatment resulted in reduced intestinal circumference leading to condensation of enteric neurons. No marked changes in neurotransmitter expression of the enteric neurons or in motor activity were noted.}}, author = {{Ekelund, Mikael and Ekelund, Mats and Qader, Saleem and Hallén, Magnus and Ekblad, Eva}}, issn = {{1095-8673}}, keywords = {{plasticity; motility; morphology; ENS; intestine; rat; TPN}}, language = {{eng}}, number = {{2}}, pages = {{187--193}}, publisher = {{Elsevier}}, series = {{Journal of Surgical Research}}, title = {{Effects of total parenteral nutrition on rat enteric nervous system, intestinal morphology, and motility.}}, url = {{http://dx.doi.org/10.1016/j.jss.2004.10.008}}, doi = {{10.1016/j.jss.2004.10.008}}, volume = {{124}}, year = {{2005}}, }