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Isolation and Purification of Mycobacterial Extracellular Vesicles

Umashankar Rao, Komal LU and Godaly, Gabriela LU orcid (2023) In Methods in molecular biology 2674. p.55-60
Abstract (Swedish)
Bacterial extracellular vesicles (EVs) contain numerous active substances that mediate bacterial interactions with their host and with other microbes. Best defined are the EVs from Gram-negative bacteria that have been shown to deliver virulence factors, modulate the immune responses, mediate antibiotic resistance, and also inhibit competitive microbes. Due to the complex cell wall structures of Gram-positive bacteria and mycobacteria, EVs from these bacteria were only recently reported. This protocol describes the isolation of EVs from mycobacteria.
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Bacterial Pathogenesis
series title
Methods in molecular biology
editor
Nordenfelt, Pontus and Collin, Mattias
volume
2674
edition
2nd
pages
55 - 60
publisher
Springer
external identifiers
  • pmid:37258959
  • scopus:85160694138
ISSN
1940-6029
1064-3745
ISBN
978-1-0716-3243-7
978-1-0716-3242-0
DOI
10.1007/978-1-0716-3243-7_3
language
Swedish
LU publication?
yes
id
f9c05ca0-5934-4db6-b024-02a668c10261
date added to LUP
2023-08-03 14:21:35
date last changed
2024-12-16 00:41:41
@inbook{f9c05ca0-5934-4db6-b024-02a668c10261,
  abstract     = {{Bacterial extracellular vesicles (EVs) contain numerous active substances that mediate bacterial interactions with their host and with other microbes. Best defined are the EVs from Gram-negative bacteria that have been shown to deliver virulence factors, modulate the immune responses, mediate antibiotic resistance, and also inhibit competitive microbes. Due to the complex cell wall structures of Gram-positive bacteria and mycobacteria, EVs from these bacteria were only recently reported. This protocol describes the isolation of EVs from mycobacteria.}},
  author       = {{Umashankar Rao, Komal and Godaly, Gabriela}},
  booktitle    = {{Bacterial Pathogenesis}},
  editor       = {{Nordenfelt, Pontus and Collin, Mattias}},
  isbn         = {{978-1-0716-3243-7}},
  issn         = {{1940-6029}},
  language     = {{swe}},
  pages        = {{55--60}},
  publisher    = {{Springer}},
  series       = {{Methods in molecular biology}},
  title        = {{Isolation and Purification of Mycobacterial Extracellular Vesicles}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-3243-7_3}},
  doi          = {{10.1007/978-1-0716-3243-7_3}},
  volume       = {{2674}},
  year         = {{2023}},
}