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Superporous agarose, a new material for chromatography

Gustavsson, Per-Erik LU and Larsson, Per-Olof LU (1996) In Journal of chromatography. A 734(2). p.231-240
Abstract
This paper reports on a new type of spherical agarose chromatography particles characterized by two sets of pores, normal diffusion pores, characteristic of all agarose materials and very wide pores, so-called superpores or flow pores. These superpores allow part of the chromatographic flow to pass through each individual particle, which gives improved mass transfer, especially in situations where diffusion is the limiting factor for the overall performance of a chromatographic separation. The particles were prepared by a double emulsification procedure. Observations under a microscope and size-exclusion chromatography were used in order to demonstrate pore flow. The chromatographic behaviour of the new particles was as efficient as that... (More)
This paper reports on a new type of spherical agarose chromatography particles characterized by two sets of pores, normal diffusion pores, characteristic of all agarose materials and very wide pores, so-called superpores or flow pores. These superpores allow part of the chromatographic flow to pass through each individual particle, which gives improved mass transfer, especially in situations where diffusion is the limiting factor for the overall performance of a chromatographic separation. The particles were prepared by a double emulsification procedure. Observations under a microscope and size-exclusion chromatography were used in order to demonstrate pore flow. The chromatographic behaviour of the new particles was as efficient as that of homogeneous particles which were several times smaller. The agarose particles were derivatized with polyethyleneimine and used for an ion-exchange chromatographic separation of three model proteins. As expected from a perfusion material, the superporous beads resolved the protein mixture more efficiently than homogeneous beads of the same size. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of chromatography. A
volume
734
issue
2
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:0029982158
ISSN
0021-9673
DOI
10.1016/0021-9673(95)01304-0
language
English
LU publication?
yes
id
fa12e1b5-8182-4a95-9729-b8d56003a5d8
date added to LUP
2024-06-10 20:38:39
date last changed
2024-07-05 04:00:56
@article{fa12e1b5-8182-4a95-9729-b8d56003a5d8,
  abstract     = {{This paper reports on a new type of spherical agarose chromatography particles characterized by two sets of pores, normal diffusion pores, characteristic of all agarose materials and very wide pores, so-called superpores or flow pores. These superpores allow part of the chromatographic flow to pass through each individual particle, which gives improved mass transfer, especially in situations where diffusion is the limiting factor for the overall performance of a chromatographic separation. The particles were prepared by a double emulsification procedure. Observations under a microscope and size-exclusion chromatography were used in order to demonstrate pore flow. The chromatographic behaviour of the new particles was as efficient as that of homogeneous particles which were several times smaller. The agarose particles were derivatized with polyethyleneimine and used for an ion-exchange chromatographic separation of three model proteins. As expected from a perfusion material, the superporous beads resolved the protein mixture more efficiently than homogeneous beads of the same size.}},
  author       = {{Gustavsson, Per-Erik and Larsson, Per-Olof}},
  issn         = {{0021-9673}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{231--240}},
  publisher    = {{Elsevier}},
  series       = {{Journal of chromatography. A}},
  title        = {{Superporous agarose, a new material for chromatography}},
  url          = {{http://dx.doi.org/10.1016/0021-9673(95)01304-0}},
  doi          = {{10.1016/0021-9673(95)01304-0}},
  volume       = {{734}},
  year         = {{1996}},
}