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Distribution of protein tracers in the nervous system of the crayfish (Astacus astacus L) following systemic and local application

Kristensson, K; Strömberg, Elisabeth; Elofsson, Rolf LU and Olsson, Yngve (1972) In Journal of Neurocytology 1. p.35-48
Abstract
A study was made on the penetration and cellular uptake of two protein tracers, albumin labelled with Evans blue (EBA) and horseradish peroxidase (HP), in the nervous system of the crayfish following systemic and local administration. Followingsystemic injection, EBA did not diffuse freely from the cerebral vessels into the brain parenchyma. When the tracers werelocally applied on the surface of the ventral nerve cord their penetration into the nervous parenchyma was to some extent restricted by the nerve sheath. However, unlike the perineurium of vertebrate peripheral nerves, which acts as an efficient diffusion barrier, the crayfish nerve sheath allowed the diffusion of small amounts of tracers into the ganglia. The tracers could more... (More)
A study was made on the penetration and cellular uptake of two protein tracers, albumin labelled with Evans blue (EBA) and horseradish peroxidase (HP), in the nervous system of the crayfish following systemic and local administration. Followingsystemic injection, EBA did not diffuse freely from the cerebral vessels into the brain parenchyma. When the tracers werelocally applied on the surface of the ventral nerve cord their penetration into the nervous parenchyma was to some extent restricted by the nerve sheath. However, unlike the perineurium of vertebrate peripheral nerves, which acts as an efficient diffusion barrier, the crayfish nerve sheath allowed the diffusion of small amounts of tracers into the ganglia. The tracers could more readily penetrate into peripheral nerves in the vicinity of ganglia. Inside the ganglion the tracers spread in extracellular spaces, between glial cell membranes and reached the neuronal surfaces. The proteins were taken up by pinocytosis in glial cells, and also in axons. (Less)
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published
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Journal of Neurocytology
volume
1
pages
35 - 48
external identifiers
  • scopus:0015365946
DOI
10.1007/BF01098644
language
English
LU publication?
yes
id
fa1c7c65-da12-4db1-acd5-d0b9f9bb289e
date added to LUP
2016-12-03 12:45:42
date last changed
2017-01-01 08:41:41
@article{fa1c7c65-da12-4db1-acd5-d0b9f9bb289e,
  abstract     = {A study was made on the penetration and cellular uptake of two protein tracers, albumin labelled with Evans blue (EBA) and horseradish peroxidase (HP), in the nervous system of the crayfish following systemic and local administration. Followingsystemic injection, EBA did not diffuse freely from the cerebral vessels into the brain parenchyma. When the tracers werelocally applied on the surface of the ventral nerve cord their penetration into the nervous parenchyma was to some extent restricted by the nerve sheath. However, unlike the perineurium of vertebrate peripheral nerves, which acts as an efficient diffusion barrier, the crayfish nerve sheath allowed the diffusion of small amounts of tracers into the ganglia. The tracers could more readily penetrate into peripheral nerves in the vicinity of ganglia. Inside the ganglion the tracers spread in extracellular spaces, between glial cell membranes and reached the neuronal surfaces. The proteins were taken up by pinocytosis in glial cells, and also in axons.},
  author       = {Kristensson, K and Strömberg, Elisabeth and Elofsson, Rolf and Olsson, Yngve},
  language     = {eng},
  pages        = {35--48},
  series       = {Journal of Neurocytology},
  title        = {Distribution of protein tracers in the nervous system of the crayfish (Astacus astacus L) following systemic and local application},
  url          = {http://dx.doi.org/10.1007/BF01098644},
  volume       = {1},
  year         = {1972},
}