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Superporous agarose as an affinity chromatography support

Gustavsson, Per-Erik LU ; Mosbach, Klaus LU ; Nilsson, Kjell LU and Larsson, Per-Olof LU (1997) In Journal of chromatography. A 776(2). p.197-203
Abstract
Superporous agarose beads were used as an affinity support in column chromatography. These beads characteristically possess two sets of pores, normal diffusion pores and flow pores, so-called superpores. The superpores, whose diameter is a substantial fraction of the particle diameter (i.e. 1/3 to 1/10 of the particle diameter), allow part of the chromatographic flow to pass through each individual bead. Consequently, significant improvement in mass transfer is observed in superporous beads as compared with homogeneous beads, especially at high flow-rates [Gustavsson and Larsson, J. Chromatogr. A, 734 (1996) 231–240.]

Superporous agarose beads and homogeneous agarose beads were each derivatized with two types of affinity ligands.... (More)
Superporous agarose beads were used as an affinity support in column chromatography. These beads characteristically possess two sets of pores, normal diffusion pores and flow pores, so-called superpores. The superpores, whose diameter is a substantial fraction of the particle diameter (i.e. 1/3 to 1/10 of the particle diameter), allow part of the chromatographic flow to pass through each individual bead. Consequently, significant improvement in mass transfer is observed in superporous beads as compared with homogeneous beads, especially at high flow-rates [Gustavsson and Larsson, J. Chromatogr. A, 734 (1996) 231–240.]

Superporous agarose beads and homogeneous agarose beads were each derivatized with two types of affinity ligands. A NAD+ analogue was used for the purification of bovine lactate dehydrogenase and protein A was used for the adsorption of rabbit IgG. The performances of superporous beads and homogeneous beads were compared. Superporous bead columns derivatized with protein A and NAD+ analogue could be operated 5 times and 3 times, respectively, as fast as corresponding homogeneous bead columns. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Agarose, superporous, Affinity, Stationary phases, LC, Flow pores, Proteins
in
Journal of chromatography. A
volume
776
issue
2
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:0030760689
ISSN
0021-9673
DOI
10.1016/S0021-9673(97)00348-8
language
English
LU publication?
yes
id
fa2bc3e3-1606-4187-95b8-1724ec2ae6d8
date added to LUP
2024-06-10 20:08:00
date last changed
2024-06-11 11:21:13
@article{fa2bc3e3-1606-4187-95b8-1724ec2ae6d8,
  abstract     = {{Superporous agarose beads were used as an affinity support in column chromatography. These beads characteristically possess two sets of pores, normal diffusion pores and flow pores, so-called superpores. The superpores, whose diameter is a substantial fraction of the particle diameter (i.e. 1/3 to 1/10 of the particle diameter), allow part of the chromatographic flow to pass through each individual bead. Consequently, significant improvement in mass transfer is observed in superporous beads as compared with homogeneous beads, especially at high flow-rates [Gustavsson and Larsson, J. Chromatogr. A, 734 (1996) 231–240.]<br/><br/>Superporous agarose beads and homogeneous agarose beads were each derivatized with two types of affinity ligands. A NAD+ analogue was used for the purification of bovine lactate dehydrogenase and protein A was used for the adsorption of rabbit IgG. The performances of superporous beads and homogeneous beads were compared. Superporous bead columns derivatized with protein A and NAD+ analogue could be operated 5 times and 3 times, respectively, as fast as corresponding homogeneous bead columns.}},
  author       = {{Gustavsson, Per-Erik and Mosbach, Klaus and Nilsson, Kjell and Larsson, Per-Olof}},
  issn         = {{0021-9673}},
  keywords     = {{Agarose, superporous; Affinity; Stationary phases, LC; Flow pores; Proteins}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{197--203}},
  publisher    = {{Elsevier}},
  series       = {{Journal of chromatography. A}},
  title        = {{Superporous agarose as an affinity chromatography support}},
  url          = {{http://dx.doi.org/10.1016/S0021-9673(97)00348-8}},
  doi          = {{10.1016/S0021-9673(97)00348-8}},
  volume       = {{776}},
  year         = {{1997}},
}