A rapid and simple ABO genotype screening method using a novel B/O2 versus A/O2 discriminating nucleotide substitution at the ABO locus
(1995) In Vox Sanguinis 69(3). p.242-247- Abstract
- An ABO genotype screening method discriminating the common alleles A1, A2, B, O1 and O2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O2 alleles. A rapid and reliable single-tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed and validated in a population of 150 Swedish blood donors. This technique is the most cost-efficient and informative ABO genotyping method reported to date.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1109592
- author
- Olsson, Martin L LU and Chester, Alan LU
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Vox Sanguinis
- volume
- 69
- issue
- 3
- pages
- 242 - 247
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:8578738
- scopus:0028844274
- ISSN
- 1423-0410
- language
- English
- LU publication?
- yes
- id
- fa562d06-2f99-4f4a-b30e-7b258326c973 (old id 1109592)
- date added to LUP
- 2016-04-01 15:23:35
- date last changed
- 2024-03-28 03:43:06
@article{fa562d06-2f99-4f4a-b30e-7b258326c973, abstract = {{An ABO genotype screening method discriminating the common alleles A1, A2, B, O1 and O2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O2 alleles. A rapid and reliable single-tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed and validated in a population of 150 Swedish blood donors. This technique is the most cost-efficient and informative ABO genotyping method reported to date.}}, author = {{Olsson, Martin L and Chester, Alan}}, issn = {{1423-0410}}, language = {{eng}}, number = {{3}}, pages = {{242--247}}, publisher = {{Wiley-Blackwell}}, series = {{Vox Sanguinis}}, title = {{A rapid and simple ABO genotype screening method using a novel B/O2 versus A/O2 discriminating nucleotide substitution at the ABO locus}}, volume = {{69}}, year = {{1995}}, }