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A new application of molecularly imprinted materials

Ye, Lei LU orcid ; Ramström, Olof ; Månsson, Mats Olle LU and Mosbach, Klaus LU (1998) In Journal of Molecular Recognition 11(1-6). p.75-78
Abstract

We have studied the possibility of shifting a thermodynamically unfavourable enzymatic equilibrium towards product formation via the addition of a highly specific adsorbent. The commercially interesting enzymatic condensation of Z-L-aspartic acid with L-phenylalanine methyl ester to the sweetener aspartame was chosen as the model system. Extremely stable and specific adsorbents for the product Z-L-Asp-L-Phe-OMe (Z-aspartame) were prepared using the emerging technique of molecular imprinting. A considerable increase (40%) in the yield of product was obtained when such adsorbents were present during the enzymatic reaction. The message of this investigation is that the use of such specific, sterilizable adsorbents should be considered for... (More)

We have studied the possibility of shifting a thermodynamically unfavourable enzymatic equilibrium towards product formation via the addition of a highly specific adsorbent. The commercially interesting enzymatic condensation of Z-L-aspartic acid with L-phenylalanine methyl ester to the sweetener aspartame was chosen as the model system. Extremely stable and specific adsorbents for the product Z-L-Asp-L-Phe-OMe (Z-aspartame) were prepared using the emerging technique of molecular imprinting. A considerable increase (40%) in the yield of product was obtained when such adsorbents were present during the enzymatic reaction. The message of this investigation is that the use of such specific, sterilizable adsorbents should be considered for enzymatic processes to increase the yield. Finally, the direct isolation of a product formed by the retrieval of the adsorbents carrying the product can be envisaged, especially if the adsorbents are magnetic.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adsorbents, Aspartame, Enzymatic synthesis, Equilibrium shifting, Molecular imprinting, Thermolysin
in
Journal of Molecular Recognition
volume
11
issue
1-6
pages
4 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0032448770
  • pmid:10076810
ISSN
0952-3499
DOI
10.1002/(SICI)1099-1352(199812)11:1/6<75::AID-JMR393>3.0.CO;2-Q
language
English
LU publication?
yes
id
fa6811f3-41b7-4bdb-b781-77d121c6d4f4
date added to LUP
2019-06-18 09:05:31
date last changed
2024-06-26 21:26:34
@article{fa6811f3-41b7-4bdb-b781-77d121c6d4f4,
  abstract     = {{<p>We have studied the possibility of shifting a thermodynamically unfavourable enzymatic equilibrium towards product formation via the addition of a highly specific adsorbent. The commercially interesting enzymatic condensation of Z-L-aspartic acid with L-phenylalanine methyl ester to the sweetener aspartame was chosen as the model system. Extremely stable and specific adsorbents for the product Z-L-Asp-L-Phe-OMe (Z-aspartame) were prepared using the emerging technique of molecular imprinting. A considerable increase (40%) in the yield of product was obtained when such adsorbents were present during the enzymatic reaction. The message of this investigation is that the use of such specific, sterilizable adsorbents should be considered for enzymatic processes to increase the yield. Finally, the direct isolation of a product formed by the retrieval of the adsorbents carrying the product can be envisaged, especially if the adsorbents are magnetic.</p>}},
  author       = {{Ye, Lei and Ramström, Olof and Månsson, Mats Olle and Mosbach, Klaus}},
  issn         = {{0952-3499}},
  keywords     = {{Adsorbents; Aspartame; Enzymatic synthesis; Equilibrium shifting; Molecular imprinting; Thermolysin}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1-6}},
  pages        = {{75--78}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Journal of Molecular Recognition}},
  title        = {{A new application of molecularly imprinted materials}},
  url          = {{http://dx.doi.org/10.1002/(SICI)1099-1352(199812)11:1/6<75::AID-JMR393>3.0.CO;2-Q}},
  doi          = {{10.1002/(SICI)1099-1352(199812)11:1/6<75::AID-JMR393>3.0.CO;2-Q}},
  volume       = {{11}},
  year         = {{1998}},
}