Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolite production
(1997) In Nature Biotechnology 15(3). p.7-244- Abstract
The gene for Vitreoscilla hemoglobin (VHb) has been introduced and expressed in Nicotiana tabaccum (tobacco). Transgenic tobacco plants expressing VHb exhibited enhanced growth, on average 80-100% more dry weight after 35 days of growth compared to wild-type controls. Furthermore, germination time is reduced from 6-8 days for wild-type tobacco to 3-4 days and the growth phase from germination to flowering was 3-5 days shorter for the VHb-expressing transgenes. Transgenic plants contained, on average, 30-40% more chlorophyll and 34% more nicotine than controls. VHb expression also resulted in an altered distribution of secondary metabolites: In the trangenic tobacco plants anabasine content was decreased 80% relative to control... (More)
The gene for Vitreoscilla hemoglobin (VHb) has been introduced and expressed in Nicotiana tabaccum (tobacco). Transgenic tobacco plants expressing VHb exhibited enhanced growth, on average 80-100% more dry weight after 35 days of growth compared to wild-type controls. Furthermore, germination time is reduced from 6-8 days for wild-type tobacco to 3-4 days and the growth phase from germination to flowering was 3-5 days shorter for the VHb-expressing transgenes. Transgenic plants contained, on average, 30-40% more chlorophyll and 34% more nicotine than controls. VHb expression also resulted in an altered distribution of secondary metabolites: In the trangenic tobacco plants anabasine content was decreased 80% relative to control plants.
(Less)
- author
- Holmberg, Niklas ; Lilius, G LU ; Bailey, James E and Bülow, L LU
- organization
- publishing date
- 1997-03
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Anabasine, Chlorophyll, Gram-Negative Aerobic Bacteria, Hemoglobins, Nicotine, Plants, Genetically Modified, Plants, Toxic, Tobacco
- in
- Nature Biotechnology
- volume
- 15
- issue
- 3
- pages
- 4 pages
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:9062923
- scopus:0030890431
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt0397-244
- language
- English
- LU publication?
- yes
- id
- fa68d394-af78-45a4-bfc4-337b6b7c7742
- date added to LUP
- 2016-04-18 16:00:57
- date last changed
- 2024-01-03 23:54:16
@article{fa68d394-af78-45a4-bfc4-337b6b7c7742,
abstract = {{<p>The gene for Vitreoscilla hemoglobin (VHb) has been introduced and expressed in Nicotiana tabaccum (tobacco). Transgenic tobacco plants expressing VHb exhibited enhanced growth, on average 80-100% more dry weight after 35 days of growth compared to wild-type controls. Furthermore, germination time is reduced from 6-8 days for wild-type tobacco to 3-4 days and the growth phase from germination to flowering was 3-5 days shorter for the VHb-expressing transgenes. Transgenic plants contained, on average, 30-40% more chlorophyll and 34% more nicotine than controls. VHb expression also resulted in an altered distribution of secondary metabolites: In the trangenic tobacco plants anabasine content was decreased 80% relative to control plants.</p>}},
author = {{Holmberg, Niklas and Lilius, G and Bailey, James E and Bülow, L}},
issn = {{1087-0156}},
keywords = {{Anabasine; Chlorophyll; Gram-Negative Aerobic Bacteria; Hemoglobins; Nicotine; Plants, Genetically Modified; Plants, Toxic; Tobacco}},
language = {{eng}},
number = {{3}},
pages = {{7--244}},
publisher = {{Nature Publishing Group}},
series = {{Nature Biotechnology}},
title = {{Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolite production}},
url = {{http://dx.doi.org/10.1038/nbt0397-244}},
doi = {{10.1038/nbt0397-244}},
volume = {{15}},
year = {{1997}},
}