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Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolite production

Holmberg, Niklas; Lilius, G LU ; Bailey, James E and Bülow, L LU (1997) In Nature Biotechnology 15(3). p.7-244
Abstract

The gene for Vitreoscilla hemoglobin (VHb) has been introduced and expressed in Nicotiana tabaccum (tobacco). Transgenic tobacco plants expressing VHb exhibited enhanced growth, on average 80-100% more dry weight after 35 days of growth compared to wild-type controls. Furthermore, germination time is reduced from 6-8 days for wild-type tobacco to 3-4 days and the growth phase from germination to flowering was 3-5 days shorter for the VHb-expressing transgenes. Transgenic plants contained, on average, 30-40% more chlorophyll and 34% more nicotine than controls. VHb expression also resulted in an altered distribution of secondary metabolites: In the trangenic tobacco plants anabasine content was decreased 80% relative to control... (More)

The gene for Vitreoscilla hemoglobin (VHb) has been introduced and expressed in Nicotiana tabaccum (tobacco). Transgenic tobacco plants expressing VHb exhibited enhanced growth, on average 80-100% more dry weight after 35 days of growth compared to wild-type controls. Furthermore, germination time is reduced from 6-8 days for wild-type tobacco to 3-4 days and the growth phase from germination to flowering was 3-5 days shorter for the VHb-expressing transgenes. Transgenic plants contained, on average, 30-40% more chlorophyll and 34% more nicotine than controls. VHb expression also resulted in an altered distribution of secondary metabolites: In the trangenic tobacco plants anabasine content was decreased 80% relative to control plants.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Anabasine, Chlorophyll, Gram-Negative Aerobic Bacteria, Hemoglobins, Nicotine, Plants, Genetically Modified, Plants, Toxic, Tobacco
in
Nature Biotechnology
volume
15
issue
3
pages
4 pages
publisher
Nature Publishing Group
external identifiers
  • scopus:0030890431
ISSN
1087-0156
DOI
10.1038/nbt0397-244
language
English
LU publication?
yes
id
fa68d394-af78-45a4-bfc4-337b6b7c7742
date added to LUP
2016-04-18 16:00:57
date last changed
2017-06-01 15:19:00
@article{fa68d394-af78-45a4-bfc4-337b6b7c7742,
  abstract     = {<p>The gene for Vitreoscilla hemoglobin (VHb) has been introduced and expressed in Nicotiana tabaccum (tobacco). Transgenic tobacco plants expressing VHb exhibited enhanced growth, on average 80-100% more dry weight after 35 days of growth compared to wild-type controls. Furthermore, germination time is reduced from 6-8 days for wild-type tobacco to 3-4 days and the growth phase from germination to flowering was 3-5 days shorter for the VHb-expressing transgenes. Transgenic plants contained, on average, 30-40% more chlorophyll and 34% more nicotine than controls. VHb expression also resulted in an altered distribution of secondary metabolites: In the trangenic tobacco plants anabasine content was decreased 80% relative to control plants.</p>},
  author       = {Holmberg, Niklas and Lilius, G and Bailey, James  E and Bülow, L},
  issn         = {1087-0156},
  keyword      = {Anabasine,Chlorophyll,Gram-Negative Aerobic Bacteria,Hemoglobins,Nicotine,Plants, Genetically Modified,Plants, Toxic,Tobacco},
  language     = {eng},
  number       = {3},
  pages        = {7--244},
  publisher    = {Nature Publishing Group},
  series       = {Nature Biotechnology},
  title        = {Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolite production},
  url          = {http://dx.doi.org/10.1038/nbt0397-244},
  volume       = {15},
  year         = {1997},
}