In vivo effects of interleukin-1/β on blood leukocytes in bb rats prone or resistant to diabetes
Jobe, Lance W. ; Vertrees, Sarah ; Wilson, Cindy A. ; Jacobs, Cindy ; Wilson, Deborah L. ; Picha, Kathleen S. ; Bakert, Paul and Lernmark, Åke LU
(1992)
In Autoimmunity
11(4).
p.233-237
- Abstract
Previous studies have determined that daily low dose injections of the potent cytokine interleukin-1β (IL-1β) decreased the frequency of insulin-dependent diabetes mellitus (IDDM) in diabetes-prone (DP) BB rats. In contrast, high dose injections induced an earlier than normal onset. In this study we tested whether the effects of daily human recombinant IL-1β injections on leukocyte subsets were associated with its modulation of IDDM onset in BB rats. Prior to the onset of IDDM in DP BB rats, high dose IL-1 β induced leukocytosis (P < 0.05), neutrophilia (P<0.01), and monocytosis (P < 0.001). At the onset of IDDM, lymphocyte (P < 0.01) and neutrophil (P<0.001) numbers were increased in high dose treated DP rats but not in... (More)
Previous studies have determined that daily low dose injections of the potent cytokine interleukin-1β (IL-1β) decreased the frequency of insulin-dependent diabetes mellitus (IDDM) in diabetes-prone (DP) BB rats. In contrast, high dose injections induced an earlier than normal onset. In this study we tested whether the effects of daily human recombinant IL-1β injections on leukocyte subsets were associated with its modulation of IDDM onset in BB rats. Prior to the onset of IDDM in DP BB rats, high dose IL-1 β induced leukocytosis (P < 0.05), neutrophilia (P<0.01), and monocytosis (P < 0.001). At the onset of IDDM, lymphocyte (P < 0.01) and neutrophil (P<0.001) numbers were increased in high dose treated DP rats but not in rats given saline or low dose IL-1β. In 60-day-old diabetes-resistant (DR) BB rats, neurophilia was induced by both low (P<0.05) and high (P<0.001) dose IL-1 β without the development of IDDM. At 130 days of age, when the rats were killed, it was discovered that 14/22 (64% IL-1β injected DR rats developed neutralizing IL-1β antibodies. Significantly lower neutrophil numbers were observed in high dose DR rats which developed IL-1β antibodies compared with those which did not (P=0.032). Thus, neutrophilia was dissociated from high IL-1β acceleration of IDDM onset. These results suggest that acceleration of diabetogenesis in DP rats by IL-1β is associated with increased prediabetic levels of monocytes at 60 days of age and of lymphocytes at onset of IDDM.
(Less)
- author
- Jobe, Lance W.
; Vertrees, Sarah
; Wilson, Cindy A.
; Jacobs, Cindy
; Wilson, Deborah L.
; Picha, Kathleen S.
; Bakert, Paul
and Lernmark, Åke
LU
- publishing date
- 1992-01-01
- type
- Contribution to journal
- publication status
- published
- keywords
- IL-1β antibodies, Insulin-dependent diabetes, Leukocytosis, Lymphocytosis, Monocytosis, Neutrophilia
- in
- Autoimmunity
- volume
- 11
- issue
- 4
- pages
- 5 pages
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:0026590557
- pmid:1581467
- ISSN
- 0891-6934
- DOI
- 10.3109/08916939209035160
- language
- English
- LU publication?
- no
- id
- fae752dc-ef53-4e14-aae1-36922ab23240
- date added to LUP
- 2019-09-11 09:33:09
- date last changed
- 2024-03-13 07:59:43
@article{fae752dc-ef53-4e14-aae1-36922ab23240,
abstract = {{<p>Previous studies have determined that daily low dose injections of the potent cytokine interleukin-1β (IL-1β) decreased the frequency of insulin-dependent diabetes mellitus (IDDM) in diabetes-prone (DP) BB rats. In contrast, high dose injections induced an earlier than normal onset. In this study we tested whether the effects of daily human recombinant IL-1β injections on leukocyte subsets were associated with its modulation of IDDM onset in BB rats. Prior to the onset of IDDM in DP BB rats, high dose IL-1 β induced leukocytosis (P < 0.05), neutrophilia (P<0.01), and monocytosis (P < 0.001). At the onset of IDDM, lymphocyte (P < 0.01) and neutrophil (P<0.001) numbers were increased in high dose treated DP rats but not in rats given saline or low dose IL-1β. In 60-day-old diabetes-resistant (DR) BB rats, neurophilia was induced by both low (P<0.05) and high (P<0.001) dose IL-1 β without the development of IDDM. At 130 days of age, when the rats were killed, it was discovered that 14/22 (64% IL-1β injected DR rats developed neutralizing IL-1β antibodies. Significantly lower neutrophil numbers were observed in high dose DR rats which developed IL-1β antibodies compared with those which did not (P=0.032). Thus, neutrophilia was dissociated from high IL-1β acceleration of IDDM onset. These results suggest that acceleration of diabetogenesis in DP rats by IL-1β is associated with increased prediabetic levels of monocytes at 60 days of age and of lymphocytes at onset of IDDM.</p>}},
author = {{Jobe, Lance W. and Vertrees, Sarah and Wilson, Cindy A. and Jacobs, Cindy and Wilson, Deborah L. and Picha, Kathleen S. and Bakert, Paul and Lernmark, Åke}},
issn = {{0891-6934}},
keywords = {{IL-1β antibodies; Insulin-dependent diabetes; Leukocytosis; Lymphocytosis; Monocytosis; Neutrophilia}},
language = {{eng}},
month = {{01}},
number = {{4}},
pages = {{233--237}},
publisher = {{Taylor & Francis}},
series = {{Autoimmunity}},
title = {{In vivo effects of interleukin-1/β on blood leukocytes in bb rats prone or resistant to diabetes}},
url = {{http://dx.doi.org/10.3109/08916939209035160}},
doi = {{10.3109/08916939209035160}},
volume = {{11}},
year = {{1992}},
}