Novel type of protein chip for multiplex detection of autoantibodies.
(2013) In Espert Review of Proteomics 10(5). p.417-420- Abstract
- Evaluation of: Akada J, Kamei S, Ito A et al. A new type of protein chip to detect hepatocellular carcinoma-related autoimmune antibodies in the sera of hepatitis C virus-positive patients. Proteome Sci. 11(1), 33 (2013). Unlocking the proteome and delivering biomarkers to the clinic will be critical for early and improved diagnosis and prognosis. Conventional protein microarrays have evolved as a promising proteomic technology with great potential for protein expression profiling in health and disease. In this study, Akada et al. explore a new type of protein chip, interfaced with a dual-color fluorescence-based read-out, for screening of autoantibodies in serum. Uniquely, the recombinant antigens were microarray adapted by molecular... (More)
- Evaluation of: Akada J, Kamei S, Ito A et al. A new type of protein chip to detect hepatocellular carcinoma-related autoimmune antibodies in the sera of hepatitis C virus-positive patients. Proteome Sci. 11(1), 33 (2013). Unlocking the proteome and delivering biomarkers to the clinic will be critical for early and improved diagnosis and prognosis. Conventional protein microarrays have evolved as a promising proteomic technology with great potential for protein expression profiling in health and disease. In this study, Akada et al. explore a new type of protein chip, interfaced with a dual-color fluorescence-based read-out, for screening of autoantibodies in serum. Uniquely, the recombinant antigens were microarray adapted by molecular design to contain a five-cysteine tag for immobilization and green fluorescent protein for detection (color 1). The engineered antigens were immobilized on in-house-designed maleimide-incorporated diamond-like carbon substrates and subsequently heat treated in a solution of denaturing and reducing agents before any specifically bound serum autoantibodies were detected (color 2). The authors used a 4-plex array targeting hepatocellular carcinoma-related autoantibodies in the sera of hepatitis C virus-positive patients as model system to demonstrate proof-of-concept. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4143746
- author
- Wingren, Christer LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Espert Review of Proteomics
- volume
- 10
- issue
- 5
- pages
- 417 - 420
- publisher
- Future Drugs Ltd
- external identifiers
-
- pmid:24088012
- wos:000330534800008
- scopus:84886062656
- pmid:24088012
- ISSN
- 1744-8387
- DOI
- 10.1586/14789450.2013.842900
- language
- English
- LU publication?
- yes
- id
- faefb9c9-45e4-4086-934b-bea6238f7ad4 (old id 4143746)
- date added to LUP
- 2016-04-01 10:28:04
- date last changed
- 2022-01-25 23:31:51
@article{faefb9c9-45e4-4086-934b-bea6238f7ad4,
abstract = {{Evaluation of: Akada J, Kamei S, Ito A et al. A new type of protein chip to detect hepatocellular carcinoma-related autoimmune antibodies in the sera of hepatitis C virus-positive patients. Proteome Sci. 11(1), 33 (2013). Unlocking the proteome and delivering biomarkers to the clinic will be critical for early and improved diagnosis and prognosis. Conventional protein microarrays have evolved as a promising proteomic technology with great potential for protein expression profiling in health and disease. In this study, Akada et al. explore a new type of protein chip, interfaced with a dual-color fluorescence-based read-out, for screening of autoantibodies in serum. Uniquely, the recombinant antigens were microarray adapted by molecular design to contain a five-cysteine tag for immobilization and green fluorescent protein for detection (color 1). The engineered antigens were immobilized on in-house-designed maleimide-incorporated diamond-like carbon substrates and subsequently heat treated in a solution of denaturing and reducing agents before any specifically bound serum autoantibodies were detected (color 2). The authors used a 4-plex array targeting hepatocellular carcinoma-related autoantibodies in the sera of hepatitis C virus-positive patients as model system to demonstrate proof-of-concept.}},
author = {{Wingren, Christer}},
issn = {{1744-8387}},
language = {{eng}},
number = {{5}},
pages = {{417--420}},
publisher = {{Future Drugs Ltd}},
series = {{Espert Review of Proteomics}},
title = {{Novel type of protein chip for multiplex detection of autoantibodies.}},
url = {{http://dx.doi.org/10.1586/14789450.2013.842900}},
doi = {{10.1586/14789450.2013.842900}},
volume = {{10}},
year = {{2013}},
}