Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices
Giacomoni, Jessica LU ; Habekost, Mette LU
; Cepeda-Prado, Efrain
LU
; Mattsson, Bengt
LU
; Rylander Ottosson, Daniella
LU
; Parmar, Malin
LU
and Kajtez, Janko
LU
(2023)
In STAR Protocols
4(1).
- Abstract
- Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/fb3af781-e293-42a0-8bc7-b112dd9b3836
- author
- Giacomoni, Jessica
LU
; Habekost, Mette
LU
; Cepeda-Prado, Efrain
LU
; Mattsson, Bengt
LU
; Rylander Ottosson, Daniella
LU
; Parmar, Malin
LU
and Kajtez, Janko
LU
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- in
- STAR Protocols
- volume
- 4
- issue
- 1
- article number
- 102041
- publisher
- Cell Press
- external identifiers
-
- pmid:36853668
- scopus:85174640568
- ISSN
- 2666-1667
- DOI
- 10.1016/j.xpro.2022.102041
- project
- Novel Strategies for Cell-based Neural Reconstruction
- language
- English
- LU publication?
- yes
- id
- fb3af781-e293-42a0-8bc7-b112dd9b3836
- date added to LUP
- 2023-02-10 15:33:48
- date last changed
- 2024-06-14 02:57:37
@article{fb3af781-e293-42a0-8bc7-b112dd9b3836,
abstract = {{Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change.}},
author = {{Giacomoni, Jessica and Habekost, Mette and Cepeda-Prado, Efrain and Mattsson, Bengt and Rylander Ottosson, Daniella and Parmar, Malin and Kajtez, Janko}},
issn = {{2666-1667}},
language = {{eng}},
number = {{1}},
publisher = {{Cell Press}},
series = {{STAR Protocols}},
title = {{Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices}},
url = {{http://dx.doi.org/10.1016/j.xpro.2022.102041}},
doi = {{10.1016/j.xpro.2022.102041}},
volume = {{4}},
year = {{2023}},
}