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Release of moth pheromone compounds from Nicotiana benthamiana upon transient expression of heterologous biosynthetic genes

Xia, Yi Han LU ; Ding, Bao Jian LU ; Dong, Shuang Lin ; Wang, Hong Lei LU ; Hofvander, Per and Löfstedt, Christer LU (2022) In BMC Biology 20(1). p.1-18
Abstract

Background: Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management. Results: In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana, including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase Atr∆11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the... (More)

Background: Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management. Results: In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer Chilo suppressalis (Walker) by heterologous expression in Saccharomyces cerevisiae and Nicotiana benthamiana, including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase Atr∆11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the major pheromone component of C. suppressalis. Both compounds were collected from the transformed N. benthamiana headspace volatiles using solid-phase microextraction. We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant. Conclusions: Our results pave the way for stable transformation of plants to be used as biological pheromone sources in different pest control strategies.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acetyltransferase, Alcohol oxidation, Fatty acyl desaturases, Fatty acyl reductase, Functional characterization, Heterologous expression systems, Pheromone-releasing plants
in
BMC Biology
volume
20
issue
1
article number
80
pages
1 - 18
publisher
BioMed Central (BMC)
external identifiers
  • pmid:35361182
  • scopus:85127377773
ISSN
1741-7007
DOI
10.1186/s12915-022-01281-8
language
English
LU publication?
yes
id
fb3e0c95-5111-4052-bb22-bb8d36f28d26
date added to LUP
2022-05-16 12:01:48
date last changed
2025-01-18 15:54:08
@article{fb3e0c95-5111-4052-bb22-bb8d36f28d26,
  abstract     = {{<p>Background: Using genetically modified plants as natural dispensers of insect pheromones may eventually become part of a novel strategy for integrated pest management. Results: In the present study, we first characterized essential functional genes for sex pheromone biosynthesis in the rice stem borer <i>Chilo suppressalis</i> (Walker) by heterologous expression in <i>Saccharomyces cerevisiae</i> and <i>Nicotiana benthamiana</i>, including two desaturase genes CsupYPAQ and CsupKPSE and a reductase gene CsupFAR2. Subsequently, we co-expressed CsupYPAQ and CsupFAR2 together with the previously characterized moth desaturase Atr∆11 in N. benthamiana. This resulted in the production of (Z)-11-hexadecenol together with (Z)-11-hexadecenal, the major pheromone component of <i>C. suppressalis</i>. Both compounds were collected from the transformed <i>N. benthamiana</i> headspace volatiles using solid-phase microextraction. We finally added the expression of a yeast acetyltransferase gene ATF1 and could then confirm also (Z)-11-hexadecenyl acetate release from the plant. Conclusions: Our results pave the way for stable transformation of plants to be used as biological pheromone sources in different pest control strategies.</p>}},
  author       = {{Xia, Yi Han and Ding, Bao Jian and Dong, Shuang Lin and Wang, Hong Lei and Hofvander, Per and Löfstedt, Christer}},
  issn         = {{1741-7007}},
  keywords     = {{Acetyltransferase; Alcohol oxidation; Fatty acyl desaturases; Fatty acyl reductase; Functional characterization; Heterologous expression systems; Pheromone-releasing plants}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{1}},
  pages        = {{1--18}},
  publisher    = {{BioMed Central (BMC)}},
  series       = {{BMC Biology}},
  title        = {{Release of moth pheromone compounds from <i>Nicotiana benthamiana</i> upon transient expression of heterologous biosynthetic genes}},
  url          = {{http://dx.doi.org/10.1186/s12915-022-01281-8}},
  doi          = {{10.1186/s12915-022-01281-8}},
  volume       = {{20}},
  year         = {{2022}},
}