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Quantitative proteomics analysis of cartilage response to mechanical injury and cytokine treatment

Wang, Yang; Li, Yang; Khabut, Areej LU ; Chubinskaya, Susan; Grodzinsky, Alan J. LU and Önnerfjord, Patrik LU (2017) In Matrix Biology 63. p.11-22
Abstract

Mechanical damage at the time of joint injury and the ensuing inflammatory response associated with elevated levels of pro-inflammatory cytokines in the synovial fluid, are reported to contribute to the progression to osteoarthritis after injury. In this exploratory study, we used a targeted proteomics approach to follow the progression of matrix degradation in response to mechanical damage and cytokine treatment of human knee cartilage explants, and thereby to study potential molecular biomarkers. This proteomics approach allowed us to unambiguously identify and quantify multiple peptides and proteins in the cartilage medium and explants upon treatment with ±. injurious compression ±. cytokines, treatments that mimic the earliest... (More)

Mechanical damage at the time of joint injury and the ensuing inflammatory response associated with elevated levels of pro-inflammatory cytokines in the synovial fluid, are reported to contribute to the progression to osteoarthritis after injury. In this exploratory study, we used a targeted proteomics approach to follow the progression of matrix degradation in response to mechanical damage and cytokine treatment of human knee cartilage explants, and thereby to study potential molecular biomarkers. This proteomics approach allowed us to unambiguously identify and quantify multiple peptides and proteins in the cartilage medium and explants upon treatment with ±. injurious compression ±. cytokines, treatments that mimic the earliest events in post-traumatic OA. We followed degradation of different protein domains, e.g., G1/G2/G3 of aggrecan, by measuring representative peptides of matrix proteins released into the medium at 7 time points throughout the 21-day culture period. COMP neo-epitopes, which were previously identified in the synovial fluid of knee injury/OA patients, were also released by these human cartilage explants treated with cyt and cyt+inj. The absence of collagen pro-peptides and elevated levels of specific COMP and COL3A1 neo-epitopes after human knee trauma may be relevant as potential biomarkers for post-traumatic OA. This model system thereby enables study of the kinetics of cartilage degradation and the identification of biomarkers within cartilage explants and those released to culture medium. Discovery proteomics revealed that candidate proteases were identified after specific treatment conditions, including MMP1, MMP-3, MMP-10 and MMP-13.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cartilage matrix, Cytokines, Mass spectrometry, Post-traumatic osteoarthritis, Proteomics
in
Matrix Biology
volume
63
pages
11 - 22
publisher
Elsevier
external identifiers
  • scopus:85009354403
  • wos:000413135300002
ISSN
0945-053X
DOI
10.1016/j.matbio.2016.12.004
language
English
LU publication?
yes
id
fb3fc69e-5c9b-4dd8-8ccf-6163c3400dfd
date added to LUP
2017-01-27 07:28:49
date last changed
2018-01-16 13:22:59
@article{fb3fc69e-5c9b-4dd8-8ccf-6163c3400dfd,
  abstract     = {<p>Mechanical damage at the time of joint injury and the ensuing inflammatory response associated with elevated levels of pro-inflammatory cytokines in the synovial fluid, are reported to contribute to the progression to osteoarthritis after injury. In this exploratory study, we used a targeted proteomics approach to follow the progression of matrix degradation in response to mechanical damage and cytokine treatment of human knee cartilage explants, and thereby to study potential molecular biomarkers. This proteomics approach allowed us to unambiguously identify and quantify multiple peptides and proteins in the cartilage medium and explants upon treatment with ±. injurious compression ±. cytokines, treatments that mimic the earliest events in post-traumatic OA. We followed degradation of different protein domains, e.g., G1/G2/G3 of aggrecan, by measuring representative peptides of matrix proteins released into the medium at 7 time points throughout the 21-day culture period. COMP neo-epitopes, which were previously identified in the synovial fluid of knee injury/OA patients, were also released by these human cartilage explants treated with cyt and cyt+inj. The absence of collagen pro-peptides and elevated levels of specific COMP and COL3A1 neo-epitopes after human knee trauma may be relevant as potential biomarkers for post-traumatic OA. This model system thereby enables study of the kinetics of cartilage degradation and the identification of biomarkers within cartilage explants and those released to culture medium. Discovery proteomics revealed that candidate proteases were identified after specific treatment conditions, including MMP1, MMP-3, MMP-10 and MMP-13.</p>},
  author       = {Wang, Yang and Li, Yang and Khabut, Areej and Chubinskaya, Susan and Grodzinsky, Alan J. and Önnerfjord, Patrik},
  issn         = {0945-053X},
  keyword      = {Cartilage matrix,Cytokines,Mass spectrometry,Post-traumatic osteoarthritis,Proteomics},
  language     = {eng},
  pages        = {11--22},
  publisher    = {Elsevier},
  series       = {Matrix Biology},
  title        = {Quantitative proteomics analysis of cartilage response to mechanical injury and cytokine treatment},
  url          = {http://dx.doi.org/10.1016/j.matbio.2016.12.004},
  volume       = {63},
  year         = {2017},
}