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Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection

Brandariz-Nuñez, Alberto; Liu, Ting; Du, Te and Evilevitch, Alex LU (2019) In eLife 8.
Abstract

Many viruses previously have been shown to have pressurized genomes inside their viral protein shell, termed the capsid. This pressure results from the tight confinement of negatively charged viral nucleic acids inside the capsid. However, the relevance of capsid pressure to viral infection has not been demonstrated. In this work, we show that the internal DNA pressure of tens of atmospheres inside a herpesvirus capsid powers ejection of the viral genome into a host cell nucleus. To our knowledge, this provides the first demonstration of a pressure-dependent mechanism of viral genome penetration into a host nucleus, leading to infection of eukaryotic cells.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cell nucleus, DNA ejection, DNA pressure, herpes capsid, herpesvirus, infectious disease, microbiology, physics of living systems, pressure-driven DNA ejection, virus
in
eLife
volume
8
publisher
eLife Sciences Publications LTD.
external identifiers
  • scopus:85071713393
ISSN
2050-084X
DOI
10.7554/eLife.47212
language
English
LU publication?
yes
id
fbc4c029-d2e5-4fb8-9690-9261517a7f3f
date added to LUP
2019-09-19 11:09:53
date last changed
2019-10-08 03:58:34
@article{fbc4c029-d2e5-4fb8-9690-9261517a7f3f,
  abstract     = {<p>Many viruses previously have been shown to have pressurized genomes inside their viral protein shell, termed the capsid. This pressure results from the tight confinement of negatively charged viral nucleic acids inside the capsid. However, the relevance of capsid pressure to viral infection has not been demonstrated. In this work, we show that the internal DNA pressure of tens of atmospheres inside a herpesvirus capsid powers ejection of the viral genome into a host cell nucleus. To our knowledge, this provides the first demonstration of a pressure-dependent mechanism of viral genome penetration into a host nucleus, leading to infection of eukaryotic cells.</p>},
  articleno    = {e47212},
  author       = {Brandariz-Nuñez, Alberto and Liu, Ting and Du, Te and Evilevitch, Alex},
  issn         = {2050-084X},
  keyword      = {cell nucleus,DNA ejection,DNA pressure,herpes capsid,herpesvirus,infectious disease,microbiology,physics of living systems,pressure-driven DNA ejection,virus},
  language     = {eng},
  month        = {08},
  publisher    = {eLife Sciences Publications LTD.},
  series       = {eLife},
  title        = {Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection},
  url          = {http://dx.doi.org/10.7554/eLife.47212},
  volume       = {8},
  year         = {2019},
}