Advanced

Cytokine evaluation in untreated and radioimmunotherapy-treated tumors in an immunocompetent rat model

Elgström, Erika LU ; Ohlsson, Tomas and Eriksson, Sophie E. LU (2017) In Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 39(4).
Abstract

The tumor microenvironment can act so as to stimulate or reject tumor cells. Among the determining factors are cytokines produced, for example, by infiltrating immune cells, tumor cells, and fibroblasts. External radiotherapy has been shown to be able to activate an immune response against tumor cells with cytokine signaling as an important part of the activation. The aim of this study was to evaluate the cytokines present in the tumor microenvironment and whether the cytokine profile changed during tumor regression induced by radioimmunotherapy with the beta emitter 177Lu. Immunocompetent rats with colon carcinoma tumors were injected with 400 MBq/kg 177Lu-mAb, and the tumors were excised after 1, 2, 3, 4, 6, or 8 days post injection... (More)

The tumor microenvironment can act so as to stimulate or reject tumor cells. Among the determining factors are cytokines produced, for example, by infiltrating immune cells, tumor cells, and fibroblasts. External radiotherapy has been shown to be able to activate an immune response against tumor cells with cytokine signaling as an important part of the activation. The aim of this study was to evaluate the cytokines present in the tumor microenvironment and whether the cytokine profile changed during tumor regression induced by radioimmunotherapy with the beta emitter 177Lu. Immunocompetent rats with colon carcinoma tumors were injected with 400 MBq/kg 177Lu-mAb, and the tumors were excised after 1, 2, 3, 4, 6, or 8 days post injection (4 rats/day on days 1-6 and 8 rats on day 8). Tumors from 10 untreated rats were used as control tissue. The tumors were divided into half: one half was prepared for cytokine analysis with a cytokine array kit and the other half was used for histological analysis. A total of 18 of the 29 cytokines evaluated were detected in this tumor model, and the majority of these act in a pro-inflammatory manner or stimulate the infiltration of immune cells. The differences between treated tumors and control tumors were small, thus the cytokine profile in the untreated tumors did not transfer to an anti-inflammatory profile during tumor regression induced by radioimmunotherapy with 177Lu. Histological evaluation demonstrated a heterogeneous pattern of ongoing cell death and the formation of granulation tissue.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
colon carcinoma, cytokines, immunocompetent rat tumor model, Lu-177, radioimmunotherapy, Tumor microenvironment
in
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
volume
39
issue
4
publisher
Springer
external identifiers
  • scopus:85018598622
  • wos:000400268800035
ISSN
1423-0380
DOI
10.1177/1010428317697550
language
English
LU publication?
yes
id
fbf0452f-ee4f-4968-974f-eec2ab617360
date added to LUP
2017-05-24 11:01:21
date last changed
2018-01-07 12:05:02
@article{fbf0452f-ee4f-4968-974f-eec2ab617360,
  abstract     = {<p>The tumor microenvironment can act so as to stimulate or reject tumor cells. Among the determining factors are cytokines produced, for example, by infiltrating immune cells, tumor cells, and fibroblasts. External radiotherapy has been shown to be able to activate an immune response against tumor cells with cytokine signaling as an important part of the activation. The aim of this study was to evaluate the cytokines present in the tumor microenvironment and whether the cytokine profile changed during tumor regression induced by radioimmunotherapy with the beta emitter 177Lu. Immunocompetent rats with colon carcinoma tumors were injected with 400 MBq/kg 177Lu-mAb, and the tumors were excised after 1, 2, 3, 4, 6, or 8 days post injection (4 rats/day on days 1-6 and 8 rats on day 8). Tumors from 10 untreated rats were used as control tissue. The tumors were divided into half: one half was prepared for cytokine analysis with a cytokine array kit and the other half was used for histological analysis. A total of 18 of the 29 cytokines evaluated were detected in this tumor model, and the majority of these act in a pro-inflammatory manner or stimulate the infiltration of immune cells. The differences between treated tumors and control tumors were small, thus the cytokine profile in the untreated tumors did not transfer to an anti-inflammatory profile during tumor regression induced by radioimmunotherapy with 177Lu. Histological evaluation demonstrated a heterogeneous pattern of ongoing cell death and the formation of granulation tissue.</p>},
  author       = {Elgström, Erika and Ohlsson, Tomas and Eriksson, Sophie E.},
  issn         = {1423-0380},
  keyword      = {colon carcinoma,cytokines,immunocompetent rat tumor model,Lu-177,radioimmunotherapy,Tumor microenvironment},
  language     = {eng},
  month        = {04},
  number       = {4},
  publisher    = {Springer},
  series       = {Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine},
  title        = {Cytokine evaluation in untreated and radioimmunotherapy-treated tumors in an immunocompetent rat model},
  url          = {http://dx.doi.org/10.1177/1010428317697550},
  volume       = {39},
  year         = {2017},
}