Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Pattern recognition receptor expression and maturation profile of dendritic cell subtypes in human tonsils and lymph nodes

Askmyr, David LU ; Abolhalaj, Milad LU ; Gomez Jimenez, David LU ; Greiff, Lennart LU ; Lindstedt, Malin LU and Lundberg, Kristina LU (2021) In Human Immunology 82(12). p.976-981
Abstract

Dendritic cells (DCs) with capacity of antigen cross-presentation are of key interest for immunotherapy against cancer as they can induce antigen-specific cytotoxic T lymphocyte (CTL) responses. This study describes frequencies of DC subtypes in human tonsils and lymph nodes, and phenotypic aspects that may be targeted by adjuvant measures. From human tonsils and neck lymph nodes, DCs were identified through flow cytometry, and subsets of plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) were investigated. Maturity status was assessed and surface receptors with CTL-promoting potentials were studied. CD123+ pDCs as well as CD1c+, CD141+, and CD1c-CD141- mDCs were detected in tonsils and... (More)

Dendritic cells (DCs) with capacity of antigen cross-presentation are of key interest for immunotherapy against cancer as they can induce antigen-specific cytotoxic T lymphocyte (CTL) responses. This study describes frequencies of DC subtypes in human tonsils and lymph nodes, and phenotypic aspects that may be targeted by adjuvant measures. From human tonsils and neck lymph nodes, DCs were identified through flow cytometry, and subsets of plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) were investigated. Maturity status was assessed and surface receptors with CTL-promoting potentials were studied. CD123+ pDCs as well as CD1c+, CD141+, and CD1c-CD141- mDCs were detected in tonsils and lymph nodes. Both sites featured a similar presence of DC subsets, with CD123+ pDC being dominant and CD141+ mDCs least frequent. Based on CD80/CD86 expression, all DC subtypes featured a low degree of maturation. Expression of pattern recognition receptors (PRRs) CD206, CD207, DC-SIGN, TLR2, and TLR4, as well as the chemokine receptor XCR1, indicated DC subset-specific receptor profiles. We conclude that tonsils and lymph nodes share common features in terms of DC subset frequency and maturation as well as PRR and XCR1 expression pattern. Our work suggests that both sites may be considered for vaccine deposition in DC-mediated immunotherapy.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cancer, Dendritic cell, Immunotherapy, PRR, Vaccination
in
Human Immunology
volume
82
issue
12
pages
976 - 981
publisher
Elsevier
external identifiers
  • scopus:85114695570
  • pmid:34511272
ISSN
0198-8859
DOI
10.1016/j.humimm.2021.08.007
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2021 The Authors
id
fbf5460b-6b83-47d8-a977-a3a7896fee5b
date added to LUP
2021-10-13 13:49:27
date last changed
2024-04-20 14:12:42
@article{fbf5460b-6b83-47d8-a977-a3a7896fee5b,
  abstract     = {{<p>Dendritic cells (DCs) with capacity of antigen cross-presentation are of key interest for immunotherapy against cancer as they can induce antigen-specific cytotoxic T lymphocyte (CTL) responses. This study describes frequencies of DC subtypes in human tonsils and lymph nodes, and phenotypic aspects that may be targeted by adjuvant measures. From human tonsils and neck lymph nodes, DCs were identified through flow cytometry, and subsets of plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) were investigated. Maturity status was assessed and surface receptors with CTL-promoting potentials were studied. CD123<sup>+</sup> pDCs as well as CD1c<sup>+</sup>, CD141<sup>+</sup>, and CD1c<sup>-</sup>CD141<sup>-</sup> mDCs were detected in tonsils and lymph nodes. Both sites featured a similar presence of DC subsets, with CD123<sup>+</sup> pDC being dominant and CD141<sup>+</sup> mDCs least frequent. Based on CD80/CD86 expression, all DC subtypes featured a low degree of maturation. Expression of pattern recognition receptors (PRRs) CD206, CD207, DC-SIGN, TLR2, and TLR4, as well as the chemokine receptor XCR1, indicated DC subset-specific receptor profiles. We conclude that tonsils and lymph nodes share common features in terms of DC subset frequency and maturation as well as PRR and XCR1 expression pattern. Our work suggests that both sites may be considered for vaccine deposition in DC-mediated immunotherapy.</p>}},
  author       = {{Askmyr, David and Abolhalaj, Milad and Gomez Jimenez, David and Greiff, Lennart and Lindstedt, Malin and Lundberg, Kristina}},
  issn         = {{0198-8859}},
  keywords     = {{Cancer; Dendritic cell; Immunotherapy; PRR; Vaccination}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{976--981}},
  publisher    = {{Elsevier}},
  series       = {{Human Immunology}},
  title        = {{Pattern recognition receptor expression and maturation profile of dendritic cell subtypes in human tonsils and lymph nodes}},
  url          = {{http://dx.doi.org/10.1016/j.humimm.2021.08.007}},
  doi          = {{10.1016/j.humimm.2021.08.007}},
  volume       = {{82}},
  year         = {{2021}},
}