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Proteomic study of non-typable Haemophilus influenzae

Thorén, Kajsa; Gustafsson, Elisabet; Clevnert, Annica; Larsson, Thomas; Bergström, Jörgen and Nilsson, Carol L LU (2002) In Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences 782(1-2). p.26-219
Abstract

Non-typable Haemophilus influenzae (NTHi) are small, gram-negative bacteria and are strictly human pathogens, causing acute otitis media, sinusitis and community-acquired pneumonia. There is no vaccine available for NTHi, as there is for H. influenzae type b. Recent advances in proteomic techniques are finding novel applications in the field of vaccinology. There are several protein separation techniques available today, each with inherent advantages and disadvantages. We employed a combined proteomics approach, including sequential extraction and analytical two-dimensional polyacrylamide electrophoresis (2D PAGE), and two-dimensional semi-preparative electrophoresis (2D PE), in order to study protein expression in the A4 NTHi strain.... (More)

Non-typable Haemophilus influenzae (NTHi) are small, gram-negative bacteria and are strictly human pathogens, causing acute otitis media, sinusitis and community-acquired pneumonia. There is no vaccine available for NTHi, as there is for H. influenzae type b. Recent advances in proteomic techniques are finding novel applications in the field of vaccinology. There are several protein separation techniques available today, each with inherent advantages and disadvantages. We employed a combined proteomics approach, including sequential extraction and analytical two-dimensional polyacrylamide electrophoresis (2D PAGE), and two-dimensional semi-preparative electrophoresis (2D PE), in order to study protein expression in the A4 NTHi strain. Although putative vaccine candidates were identified with both techniques, 11 of 15 proteins identified using the 2D PE approach were not identified by 2D PAGE, demonstrating the complementarily of the two methods.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Bacterial Proteins, Electrophoresis, Gel, Two-Dimensional, Haemophilus influenzae, Mass Spectrometry, Proteomics, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
volume
782
issue
1-2
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:0037176204
ISSN
1570-0232
language
English
LU publication?
no
id
fc3e4d3f-eec5-4f6b-8f7e-bed105dc5dde
date added to LUP
2017-05-16 10:39:30
date last changed
2017-05-31 12:11:07
@article{fc3e4d3f-eec5-4f6b-8f7e-bed105dc5dde,
  abstract     = {<p>Non-typable Haemophilus influenzae (NTHi) are small, gram-negative bacteria and are strictly human pathogens, causing acute otitis media, sinusitis and community-acquired pneumonia. There is no vaccine available for NTHi, as there is for H. influenzae type b. Recent advances in proteomic techniques are finding novel applications in the field of vaccinology. There are several protein separation techniques available today, each with inherent advantages and disadvantages. We employed a combined proteomics approach, including sequential extraction and analytical two-dimensional polyacrylamide electrophoresis (2D PAGE), and two-dimensional semi-preparative electrophoresis (2D PE), in order to study protein expression in the A4 NTHi strain. Although putative vaccine candidates were identified with both techniques, 11 of 15 proteins identified using the 2D PE approach were not identified by 2D PAGE, demonstrating the complementarily of the two methods.</p>},
  author       = {Thorén, Kajsa and Gustafsson, Elisabet and Clevnert, Annica and Larsson, Thomas and Bergström, Jörgen and Nilsson, Carol L},
  issn         = {1570-0232},
  keyword      = {Bacterial Proteins,Electrophoresis, Gel, Two-Dimensional,Haemophilus influenzae,Mass Spectrometry,Proteomics,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {12},
  number       = {1-2},
  pages        = {26--219},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences},
  title        = {Proteomic study of non-typable Haemophilus influenzae},
  volume       = {782},
  year         = {2002},
}