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Biomarker selection for detection of occult tumour cells in lymph nodes of colorectal cancer patients using real-time quantitative RT-PCR

Ohlsson, L.; Hammarstrom, M-L; Israelsson, A.; Naslund, L.; Oberg, A.; Lindmark, Gudrun LU and Hammarstrom, S. (2006) In British Journal of Cancer 95(2). p.218-225
Abstract
Accurate identification of lymph node involvement is critical for successful treatment of patients with colorectal carcinoma ( CRC). Real- time quantitative RT - PCR with a specific probe and RNA copy standard for biomarker mRNA has proven very powerful for detection of disseminated tumour cells. Which properties of biomarker mRNAs are important for identification of disseminated CRC cells? Seven biomarker candidates, CEA, CEACAMI- S/ L, CEACAM6, CEACAM7- 1/ 2, MUC2, MMP7 and CK20, were compared in a test- set of lymph nodes from 51 CRC patients ( Dukes' A - D) and 10 controls. Normal colon epithelial cells, primary tumours, and different immune cells were also analysed. The biomarkers were ranked according to: ( 1) detection of... (More)
Accurate identification of lymph node involvement is critical for successful treatment of patients with colorectal carcinoma ( CRC). Real- time quantitative RT - PCR with a specific probe and RNA copy standard for biomarker mRNA has proven very powerful for detection of disseminated tumour cells. Which properties of biomarker mRNAs are important for identification of disseminated CRC cells? Seven biomarker candidates, CEA, CEACAMI- S/ L, CEACAM6, CEACAM7- 1/ 2, MUC2, MMP7 and CK20, were compared in a test- set of lymph nodes from 51 CRC patients ( Dukes' A - D) and 10 controls. Normal colon epithelial cells, primary tumours, and different immune cells were also analysed. The biomarkers were ranked according to: ( 1) detection of haematoxylin/ eosin positive nodes, ( 2) detection of Dukes' A and B patients, who developed metastases during a 54 months follow- up period and ( 3) identification of patients with Dukes' C and D tumours using the highest value of control nodes as cutoff. The following properties appear to be of importance; ( a) no expression in immune cells, ( b) relatively high and constant expression in tumour tissue irrespective of Dukes' stage and ( c) no or weak downregulation in tumours compared to normal tissue. CEA fulfilled these criteria best, followed by CK20 and MUC2. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
mucin 2 (MUC2), carcinoembryonic antigen (CEA), CEA cell adhesion molecule (CEACAM), matrix metalloproteinase 7 (MMP7), cytokeratin 20 (CK20)
in
British Journal of Cancer
volume
95
issue
2
pages
218 - 225
publisher
Nature Publishing Group
external identifiers
  • pmid:16755296
  • wos:000239118200013
  • scopus:33745940506
ISSN
1532-1827
DOI
10.1038/sj.bjc.6603206
language
English
LU publication?
yes
id
fc916ba9-ed8c-4743-a2e1-0389f380f12c (old id 401419)
date added to LUP
2007-10-13 14:52:32
date last changed
2019-10-01 01:25:36
@article{fc916ba9-ed8c-4743-a2e1-0389f380f12c,
  abstract     = {Accurate identification of lymph node involvement is critical for successful treatment of patients with colorectal carcinoma ( CRC). Real- time quantitative RT - PCR with a specific probe and RNA copy standard for biomarker mRNA has proven very powerful for detection of disseminated tumour cells. Which properties of biomarker mRNAs are important for identification of disseminated CRC cells? Seven biomarker candidates, CEA, CEACAMI- S/ L, CEACAM6, CEACAM7- 1/ 2, MUC2, MMP7 and CK20, were compared in a test- set of lymph nodes from 51 CRC patients ( Dukes' A - D) and 10 controls. Normal colon epithelial cells, primary tumours, and different immune cells were also analysed. The biomarkers were ranked according to: ( 1) detection of haematoxylin/ eosin positive nodes, ( 2) detection of Dukes' A and B patients, who developed metastases during a 54 months follow- up period and ( 3) identification of patients with Dukes' C and D tumours using the highest value of control nodes as cutoff. The following properties appear to be of importance; ( a) no expression in immune cells, ( b) relatively high and constant expression in tumour tissue irrespective of Dukes' stage and ( c) no or weak downregulation in tumours compared to normal tissue. CEA fulfilled these criteria best, followed by CK20 and MUC2.},
  author       = {Ohlsson, L. and Hammarstrom, M-L and Israelsson, A. and Naslund, L. and Oberg, A. and Lindmark, Gudrun and Hammarstrom, S.},
  issn         = {1532-1827},
  keyword      = {mucin 2 (MUC2),carcinoembryonic antigen (CEA),CEA cell adhesion molecule (CEACAM),matrix metalloproteinase 7 (MMP7),cytokeratin 20 (CK20)},
  language     = {eng},
  number       = {2},
  pages        = {218--225},
  publisher    = {Nature Publishing Group},
  series       = {British Journal of Cancer},
  title        = {Biomarker selection for detection of occult tumour cells in lymph nodes of colorectal cancer patients using real-time quantitative RT-PCR},
  url          = {http://dx.doi.org/10.1038/sj.bjc.6603206},
  volume       = {95},
  year         = {2006},
}