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Regenerating peripheral nerves release a diffusable protein kinase

Remgård, Pär and Ekström, Per LU (1994) In Neuroscience Research Communications 14(2). p.101-108
Abstract

Using [γ32P]ATP and 1-dimensional electrophoresis this report shows that a protein kinase is released in the culture medium from adult frog sciatic nerves during regeneration in vitro. The kinase, which phosphorylated serine and to some extent threonine residues, was released from non-neuronal cells. It showed an increased activity during the 3rd to 6th day after injury, coinciding with the injury-induced proliferation of non-neuronal cells. When the proliferation was inhibited by a mitotic agent, the activity of the protein kinase was lowered, further linking it to the division of cells.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Nerve, Protein kinase, Regeneration, Release, Schwann cells
in
Neuroscience Research Communications
volume
14
issue
2
pages
8 pages
publisher
John Wiley and Sons Inc.
external identifiers
  • scopus:0028216324
ISSN
0893-6609
language
English
LU publication?
no
id
fcad6aa9-d6fe-4c49-ac67-d27b5de23cfb
date added to LUP
2016-12-07 14:46:48
date last changed
2017-01-27 15:24:28
@article{fcad6aa9-d6fe-4c49-ac67-d27b5de23cfb,
  abstract     = {<p>Using [γ<sup>32</sup>P]ATP and 1-dimensional electrophoresis this report shows that a protein kinase is released in the culture medium from adult frog sciatic nerves during regeneration in vitro. The kinase, which phosphorylated serine and to some extent threonine residues, was released from non-neuronal cells. It showed an increased activity during the 3rd to 6th day after injury, coinciding with the injury-induced proliferation of non-neuronal cells. When the proliferation was inhibited by a mitotic agent, the activity of the protein kinase was lowered, further linking it to the division of cells.</p>},
  author       = {Remgård, Pär and Ekström, Per},
  issn         = {0893-6609},
  keyword      = {Nerve,Protein kinase,Regeneration,Release,Schwann cells},
  language     = {eng},
  number       = {2},
  pages        = {101--108},
  publisher    = {John Wiley and Sons Inc.},
  series       = {Neuroscience Research Communications},
  title        = {Regenerating peripheral nerves release a diffusable protein kinase},
  volume       = {14},
  year         = {1994},
}