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Differential inhibition of receptor activation by two mouse monoclonal antibodies specific for the human leukotriene B(4) receptor, BLT(1).

Sabirsh, Alan LU ; Pettersson, Annika LU ; Boketoft, Åke LU ; Kotarsky, Knut LU and Owman, Christer LU (2003) In International Immunopharmacology 3(13-14). p.1829-1839
Abstract
The inflammatory mediator leukotriene B(4) (LTB(4)) binds to and activates a G-protein-coupled receptor named BLT(1). We have previously produced two monoclonal antibodies, named 7B1 and 14F11, that bind specifically to this receptor. Using a HeLa cell line expressing human BLT(1), we find that both antibodies inhibit LTB(4)-induced calcium release, and activation of a MAP-kinase-sensitive luciferase reporter system. The normal chemotactic movement of polymorphonuclear cells towards higher LTB(4) concentrations was also strongly inhibited by both antibodies. Neither antibody was found to activate BLT(1), and experiments using cyclic peptide fragments of the BLT(1) n-terminal and extracellular loops showed that these antibodies bind only to... (More)
The inflammatory mediator leukotriene B(4) (LTB(4)) binds to and activates a G-protein-coupled receptor named BLT(1). We have previously produced two monoclonal antibodies, named 7B1 and 14F11, that bind specifically to this receptor. Using a HeLa cell line expressing human BLT(1), we find that both antibodies inhibit LTB(4)-induced calcium release, and activation of a MAP-kinase-sensitive luciferase reporter system. The normal chemotactic movement of polymorphonuclear cells towards higher LTB(4) concentrations was also strongly inhibited by both antibodies. Neither antibody was found to activate BLT(1), and experiments using cyclic peptide fragments of the BLT(1) n-terminal and extracellular loops showed that these antibodies bind only to complex epitopes in the tertiary, membrane bound, conformation of the receptor protein. In ligand binding experiments, 7B1 was found to be a competitive antagonist, while 14F11 was a noncompetitive antagonist that inhibited receptor activation, but not agonist (LTB(4)) binding. 14F11 will be a useful tool for studying the mechanisms of receptor activation. (Less)
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type
Contribution to journal
publication status
published
subject
in
International Immunopharmacology
volume
3
issue
13-14
pages
1829 - 1839
publisher
Elsevier
external identifiers
  • pmid:14636832
  • wos:000187038600011
  • scopus:0242543343
ISSN
1878-1705
DOI
10.1016/j.intimp.2003.08.009
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Molecular Neurobiology (0131000140), Mucosal Immunology (013212072), Drug Target Discovery (013212045), Department of Experimental Medical Science (013210000)
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fcf92e95-5060-429e-b7a0-fe625fdbac9d (old id 118663)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14636832&dopt=Abstract
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2016-04-01 12:30:46
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2026-02-19 10:44:48
@article{fcf92e95-5060-429e-b7a0-fe625fdbac9d,
  abstract     = {{The inflammatory mediator leukotriene B(4) (LTB(4)) binds to and activates a G-protein-coupled receptor named BLT(1). We have previously produced two monoclonal antibodies, named 7B1 and 14F11, that bind specifically to this receptor. Using a HeLa cell line expressing human BLT(1), we find that both antibodies inhibit LTB(4)-induced calcium release, and activation of a MAP-kinase-sensitive luciferase reporter system. The normal chemotactic movement of polymorphonuclear cells towards higher LTB(4) concentrations was also strongly inhibited by both antibodies. Neither antibody was found to activate BLT(1), and experiments using cyclic peptide fragments of the BLT(1) n-terminal and extracellular loops showed that these antibodies bind only to complex epitopes in the tertiary, membrane bound, conformation of the receptor protein. In ligand binding experiments, 7B1 was found to be a competitive antagonist, while 14F11 was a noncompetitive antagonist that inhibited receptor activation, but not agonist (LTB(4)) binding. 14F11 will be a useful tool for studying the mechanisms of receptor activation.}},
  author       = {{Sabirsh, Alan and Pettersson, Annika and Boketoft, Åke and Kotarsky, Knut and Owman, Christer}},
  issn         = {{1878-1705}},
  language     = {{eng}},
  number       = {{13-14}},
  pages        = {{1829--1839}},
  publisher    = {{Elsevier}},
  series       = {{International Immunopharmacology}},
  title        = {{Differential inhibition of receptor activation by two mouse monoclonal antibodies specific for the human leukotriene B(4) receptor, BLT(1).}},
  url          = {{http://dx.doi.org/10.1016/j.intimp.2003.08.009}},
  doi          = {{10.1016/j.intimp.2003.08.009}},
  volume       = {{3}},
  year         = {{2003}},
}