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Single molecule tracking fluorescence microscopy in mitochondria reveals highly dynamic but confined movement of Tom40

Kuzmenko, Anton ; Tankov, Stoyan ; English, Brian P. ; Tarassov, Ivan ; Tenson, Tanel ; Kamenski, Piotr ; Elf, Johan LU and Hauryliuk, Vasili LU orcid (2011) In Scientific Reports 1.
Abstract

Tom40 is an integral protein of the mitochondrial outer membrane, which as the central component of the Translocase of the Outer Membrane (TOM) complex forms a channel for protein import. We characterize the diffusion properties of individual Tom40 molecules fused to the photoconvertable fluorescent protein Dendra2 with millisecond temporal resolution. By imaging individual Tom40 molecules in intact isolated yeast mitochondria using photoactivated localization microscopy with sub-diffraction limited spatial precision, we demonstrate that Tom40 movement in the outer mitochondrial membrane is highly dynamic but confined in nature, suggesting anchoring of the TOM complex as a whole.

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author
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publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
1
article number
195
publisher
Nature Publishing Group
external identifiers
  • scopus:84859735334
  • pmid:22355710
ISSN
2045-2322
DOI
10.1038/srep00195
language
English
LU publication?
no
additional info
Funding Information: We are grateful to Trevor Lithgow for providing yeast strains and plasmids, Fredrik Persson for helpful discussions, Michael Vyssokih for help with oxymetry, Dmitry Chudakov for sharing the pDendra2 plasmid and Arash Sanamrad for sharing IgorPro scripts for data analysis. JE is supported by the European Research Council, the Foundation for Strategic Research, the Swedish Research Council and the Knut and Alice Wallenberg Foundation. BPE is supported by a Human Frontier Science Program fellowship. VH and TT acknowledge support from the Estonian Science Foundation and the European Regional Development Fund through the Center of Excellence in Chemical Biology. PK and AK are supported by Russian Foundation for Basic Research, Ministry of Education and Science of Russia, the President of Russian Federation Grant for Young Researchers and funds from International Associated Laboratory ‘‘RNA-mitocure’’. AK and ST were supported by SA Archimedes foundation. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.
id
fd05d725-5cb1-402e-a387-21c6903ee74b
date added to LUP
2021-09-24 20:46:34
date last changed
2024-10-06 05:28:04
@article{fd05d725-5cb1-402e-a387-21c6903ee74b,
  abstract     = {{<p>Tom40 is an integral protein of the mitochondrial outer membrane, which as the central component of the Translocase of the Outer Membrane (TOM) complex forms a channel for protein import. We characterize the diffusion properties of individual Tom40 molecules fused to the photoconvertable fluorescent protein Dendra2 with millisecond temporal resolution. By imaging individual Tom40 molecules in intact isolated yeast mitochondria using photoactivated localization microscopy with sub-diffraction limited spatial precision, we demonstrate that Tom40 movement in the outer mitochondrial membrane is highly dynamic but confined in nature, suggesting anchoring of the TOM complex as a whole.</p>}},
  author       = {{Kuzmenko, Anton and Tankov, Stoyan and English, Brian P. and Tarassov, Ivan and Tenson, Tanel and Kamenski, Piotr and Elf, Johan and Hauryliuk, Vasili}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Single molecule tracking fluorescence microscopy in mitochondria reveals highly dynamic but confined movement of Tom40}},
  url          = {{http://dx.doi.org/10.1038/srep00195}},
  doi          = {{10.1038/srep00195}},
  volume       = {{1}},
  year         = {{2011}},
}