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Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection

Bontidean, Ibolya LU ; Ahlqvist, Josefin LU ; Mulchandani, Ashok ; Chen, Wilfred ; Bae, Weon ; Mehra, Rajesh K ; Mortari, Alessia LU and Csöregi, Elisabeth LU (2003) In Biosensors & Bioelectronics 18(5-6). p.547-553
Abstract
A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)20Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2+, Cd2+, Pb2+, Cu2+ and Zn2+ ions in concentration range of 100 fM–10 mM, and the order of sensitivity was SZn>SCu>SHg>>SCdSPb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biosensors, Heavy metals, Phytochelatins, Capacitance
in
Biosensors & Bioelectronics
volume
18
issue
5-6
pages
547 - 553
publisher
Elsevier
external identifiers
  • wos:000182790500007
  • pmid:12706561
  • scopus:0037403268
ISSN
1873-4235
DOI
10.1016/S0956-5663(03)00026-5
language
English
LU publication?
yes
id
fd5edfe7-fb23-409c-aecf-0cd462692678 (old id 129098)
date added to LUP
2016-04-01 16:41:39
date last changed
2022-04-22 23:50:28
@article{fd5edfe7-fb23-409c-aecf-0cd462692678,
  abstract     = {{A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)20Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2+, Cd2+, Pb2+, Cu2+ and Zn2+ ions in concentration range of 100 fM–10 mM, and the order of sensitivity was SZn>SCu>SHg>>SCdSPb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.}},
  author       = {{Bontidean, Ibolya and Ahlqvist, Josefin and Mulchandani, Ashok and Chen, Wilfred and Bae, Weon and Mehra, Rajesh K and Mortari, Alessia and Csöregi, Elisabeth}},
  issn         = {{1873-4235}},
  keywords     = {{Biosensors; Heavy metals; Phytochelatins; Capacitance}},
  language     = {{eng}},
  number       = {{5-6}},
  pages        = {{547--553}},
  publisher    = {{Elsevier}},
  series       = {{Biosensors & Bioelectronics}},
  title        = {{Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection}},
  url          = {{http://dx.doi.org/10.1016/S0956-5663(03)00026-5}},
  doi          = {{10.1016/S0956-5663(03)00026-5}},
  volume       = {{18}},
  year         = {{2003}},
}