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Supporting Design and Control of a Reversed-Phase Chromatography Step by Mechanistic Modeling

Westerberg, Karin LU ; Borg, Niklas LU ; Andersson, Niklas LU orcid and Nilsson, Bernt LU orcid (2012) In Chemical Engineering & Technology 35(1). p.169-175
Abstract
There is a need to design and understand control strategies for biopharmaceutical processes. The control strategy will influence the design space and process performance and should be chosen together with the operating point and design space. Mechanistic modeling is used to design and evaluate control strategies for product pooling in preparative chromatography. A kinetic dispersive model was calibrated to reversed-phase chromatography experiments. Uncorrelated process disturbances were introduced to optimized operating points, and the effects of disturbances were evaluated and reduced by control strategies. Pooling control was implemented at a fixed UV absorbance or as a function of retention volume and UV peak height. The higher... (More)
There is a need to design and understand control strategies for biopharmaceutical processes. The control strategy will influence the design space and process performance and should be chosen together with the operating point and design space. Mechanistic modeling is used to design and evaluate control strategies for product pooling in preparative chromatography. A kinetic dispersive model was calibrated to reversed-phase chromatography experiments. Uncorrelated process disturbances were introduced to optimized operating points, and the effects of disturbances were evaluated and reduced by control strategies. Pooling control was implemented at a fixed UV absorbance or as a function of retention volume and UV peak height. The higher flexibility of the second control strategy decreased the loss of yield and productivity caused by the disturbances. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Design space, Preparative chromatography, Process control, Quality by, design, Reversed-phase chromatography
in
Chemical Engineering & Technology
volume
35
issue
1
pages
169 - 175
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000298540200016
  • scopus:84855166398
ISSN
1521-4125
DOI
10.1002/ceat.201000505
language
English
LU publication?
yes
id
fdd26c44-7ab6-4221-a0bb-5968543174b6 (old id 2333853)
date added to LUP
2016-04-01 11:16:42
date last changed
2025-04-04 14:14:23
@article{fdd26c44-7ab6-4221-a0bb-5968543174b6,
  abstract     = {{There is a need to design and understand control strategies for biopharmaceutical processes. The control strategy will influence the design space and process performance and should be chosen together with the operating point and design space. Mechanistic modeling is used to design and evaluate control strategies for product pooling in preparative chromatography. A kinetic dispersive model was calibrated to reversed-phase chromatography experiments. Uncorrelated process disturbances were introduced to optimized operating points, and the effects of disturbances were evaluated and reduced by control strategies. Pooling control was implemented at a fixed UV absorbance or as a function of retention volume and UV peak height. The higher flexibility of the second control strategy decreased the loss of yield and productivity caused by the disturbances.}},
  author       = {{Westerberg, Karin and Borg, Niklas and Andersson, Niklas and Nilsson, Bernt}},
  issn         = {{1521-4125}},
  keywords     = {{Design space; Preparative chromatography; Process control; Quality by; design; Reversed-phase chromatography}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{169--175}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Chemical Engineering & Technology}},
  title        = {{Supporting Design and Control of a Reversed-Phase Chromatography Step by Mechanistic Modeling}},
  url          = {{http://dx.doi.org/10.1002/ceat.201000505}},
  doi          = {{10.1002/ceat.201000505}},
  volume       = {{35}},
  year         = {{2012}},
}