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Differential expression of proteins related to smooth muscle cells and myofibroblasts in human thoracic aortic aneurysm

Forte, Amalia; Della Corte, Alessandro; Grossi, Mario LU ; Bancone, Ciro; Maiello, Ciro; Galderisi, Umberto and Cipollaro, Marilena (2013) In Histology and Histopathology 28(6). p.795-803
Abstract
Objectives: Increasing knowledge is required for a better comprehension of the etiology of thoracic aortic aneurysm (TAA). The aim of this study was to highlight the modulations in vascular cell phenotypes, including myofibroblasts (MFs), in human TAA specimens compared to healthy aortas. Methods: histology, RT-PCR and immunohistochemical analysis of a panel of molecules, including EDA Fibronectin (Fn), smoothelin, CD34 and alpha-smooth muscle actin (alpha-SMA), selected on the basis of their informative potential as markers of smooth muscle cells (SMCs) and MF phenotypic modulation, were performed on all samples. Results: The media of TAAs was characterized by the absence of smoothelin, the unaltered expression of alpha-SMA accompanied by... (More)
Objectives: Increasing knowledge is required for a better comprehension of the etiology of thoracic aortic aneurysm (TAA). The aim of this study was to highlight the modulations in vascular cell phenotypes, including myofibroblasts (MFs), in human TAA specimens compared to healthy aortas. Methods: histology, RT-PCR and immunohistochemical analysis of a panel of molecules, including EDA Fibronectin (Fn), smoothelin, CD34 and alpha-smooth muscle actin (alpha-SMA), selected on the basis of their informative potential as markers of smooth muscle cells (SMCs) and MF phenotypic modulation, were performed on all samples. Results: The media of TAAs was characterized by the absence of smoothelin, the unaltered expression of alpha-SMA accompanied by an alteration of its distribution pattern, and by the activated expression of the ED-A isoform of Fn. We found a concentration of round-shaped cells exclusively in the adventitia and in the perivascular tissue of TAAs, also rich in vasa vasorum, largely expressing alpha-SMA, while a sub-population also expressed ED-A Fn and CD34. CD34 was expressed by several cells in the intima of TAAs, together with cells expressing cytoplasmatic EDA Fn and alpha-SMA in comparison to healthy aortas. Conclusion: TAA specimens show an altered expression and localization of SMC and MF differentiation markers in comparison to healthy aortas, with possible implications on remodeling. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Thoracic aortic aneurysm, Myofibroblasts, Fibronectin, CD34, Smoothelin, Alpha-smooth muscle actin
in
Histology and Histopathology
volume
28
issue
6
pages
795 - 803
publisher
Histology and Histopathology
external identifiers
  • wos:000318267600013
  • scopus:84878971384
ISSN
1699-5848
language
English
LU publication?
yes
id
fdd9508a-5073-4cf0-a455-989c22e8f12b (old id 3843138)
date added to LUP
2013-07-01 07:00:38
date last changed
2019-06-04 02:08:38
@article{fdd9508a-5073-4cf0-a455-989c22e8f12b,
  abstract     = {Objectives: Increasing knowledge is required for a better comprehension of the etiology of thoracic aortic aneurysm (TAA). The aim of this study was to highlight the modulations in vascular cell phenotypes, including myofibroblasts (MFs), in human TAA specimens compared to healthy aortas. Methods: histology, RT-PCR and immunohistochemical analysis of a panel of molecules, including EDA Fibronectin (Fn), smoothelin, CD34 and alpha-smooth muscle actin (alpha-SMA), selected on the basis of their informative potential as markers of smooth muscle cells (SMCs) and MF phenotypic modulation, were performed on all samples. Results: The media of TAAs was characterized by the absence of smoothelin, the unaltered expression of alpha-SMA accompanied by an alteration of its distribution pattern, and by the activated expression of the ED-A isoform of Fn. We found a concentration of round-shaped cells exclusively in the adventitia and in the perivascular tissue of TAAs, also rich in vasa vasorum, largely expressing alpha-SMA, while a sub-population also expressed ED-A Fn and CD34. CD34 was expressed by several cells in the intima of TAAs, together with cells expressing cytoplasmatic EDA Fn and alpha-SMA in comparison to healthy aortas. Conclusion: TAA specimens show an altered expression and localization of SMC and MF differentiation markers in comparison to healthy aortas, with possible implications on remodeling.},
  author       = {Forte, Amalia and Della Corte, Alessandro and Grossi, Mario and Bancone, Ciro and Maiello, Ciro and Galderisi, Umberto and Cipollaro, Marilena},
  issn         = {1699-5848},
  keyword      = {Thoracic aortic aneurysm,Myofibroblasts,Fibronectin,CD34,Smoothelin,Alpha-smooth muscle actin},
  language     = {eng},
  number       = {6},
  pages        = {795--803},
  publisher    = {Histology and Histopathology},
  series       = {Histology and Histopathology},
  title        = {Differential expression of proteins related to smooth muscle cells and myofibroblasts in human thoracic aortic aneurysm},
  volume       = {28},
  year         = {2013},
}