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Genetic Determinants of Circulating Estrogen Levels and Evidence of a Causal Effect of Estradiol on Bone Density in Men

Eriksson, Anna L.; Perry, John R.B.; Coviello, Andrea D.; Delgado, Graciela E.; Ferrucci, Luigi; Hoffman, Andrew R.; Huhtaniemi, Ilpo T. LU ; Ikram, M. Arfan; Karlsson, Magnus K. LU and Kleber, Marcus E., et al. (2018) In The Journal of clinical endocrinology and metabolism 103(3). p.991-1004
Abstract

Context: Serum estradiol (E2) and estrone (E1) levels exhibit substantial heritability.

Objective: To investigate the genetic regulation of serum E2 and E1 in men.

Design, Setting, and Participants: Genome-wide association study in 11,097 men of European origin from nine epidemiological cohorts.

Main Outcome Measures: Genetic determinants of serum E2 and E1 levels.

Results: Variants in/near CYP19A1 demonstrated the strongest evidence for association with E2, resolving to three independent signals. Two additional independent signals were found on the X chromosome; FAMily with sequence similarity 9, member B (FAM9B), rs5934505 (P = 3.4 × 10-8) and Xq27.3, rs5951794 (P = 3.1 × 10-10). E1 signals were found in... (More)

Context: Serum estradiol (E2) and estrone (E1) levels exhibit substantial heritability.

Objective: To investigate the genetic regulation of serum E2 and E1 in men.

Design, Setting, and Participants: Genome-wide association study in 11,097 men of European origin from nine epidemiological cohorts.

Main Outcome Measures: Genetic determinants of serum E2 and E1 levels.

Results: Variants in/near CYP19A1 demonstrated the strongest evidence for association with E2, resolving to three independent signals. Two additional independent signals were found on the X chromosome; FAMily with sequence similarity 9, member B (FAM9B), rs5934505 (P = 3.4 × 10-8) and Xq27.3, rs5951794 (P = 3.1 × 10-10). E1 signals were found in CYP19A1 (rs2899472, P = 5.5 × 10-23), in Tripartite motif containing 4 (TRIM4; rs17277546, P = 5.8 × 10-14), and CYP11B1/B2 (rs10093796, P = 1.2 × 10-8). E2 signals in CYP19A1 and FAM9B were associated with bone mineral density (BMD). Mendelian randomization analysis suggested a causal effect of serum E2 on BMD in men. A 1 pg/mL genetically increased E2 was associated with a 0.048 standard deviation increase in lumbar spine BMD (P = 2.8 × 10-12). In men and women combined, CYP19A1 alleles associated with higher E2 levels were associated with lower degrees of insulin resistance.

Conclusions: Our findings confirm that CYP19A1 is an important genetic regulator of E2 and E1 levels and strengthen the causal importance of E2 for bone health in men. We also report two independent loci on the X-chromosome for E2, and one locus each in TRIM4 and CYP11B1/B2, for E1.

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@article{fe246f0d-deaa-4c52-9748-ad85ab4359cf,
  abstract     = {<p>Context: Serum estradiol (E2) and estrone (E1) levels exhibit substantial heritability.</p><p>Objective: To investigate the genetic regulation of serum E2 and E1 in men.</p><p>Design, Setting, and Participants: Genome-wide association study in 11,097 men of European origin from nine epidemiological cohorts.</p><p>Main Outcome Measures: Genetic determinants of serum E2 and E1 levels.</p><p>Results: Variants in/near CYP19A1 demonstrated the strongest evidence for association with E2, resolving to three independent signals. Two additional independent signals were found on the X chromosome; FAMily with sequence similarity 9, member B (FAM9B), rs5934505 (P = 3.4 × 10-8) and Xq27.3, rs5951794 (P = 3.1 × 10-10). E1 signals were found in CYP19A1 (rs2899472, P = 5.5 × 10-23), in Tripartite motif containing 4 (TRIM4; rs17277546, P = 5.8 × 10-14), and CYP11B1/B2 (rs10093796, P = 1.2 × 10-8). E2 signals in CYP19A1 and FAM9B were associated with bone mineral density (BMD). Mendelian randomization analysis suggested a causal effect of serum E2 on BMD in men. A 1 pg/mL genetically increased E2 was associated with a 0.048 standard deviation increase in lumbar spine BMD (P = 2.8 × 10-12). In men and women combined, CYP19A1 alleles associated with higher E2 levels were associated with lower degrees of insulin resistance.</p><p>Conclusions: Our findings confirm that CYP19A1 is an important genetic regulator of E2 and E1 levels and strengthen the causal importance of E2 for bone health in men. We also report two independent loci on the X-chromosome for E2, and one locus each in TRIM4 and CYP11B1/B2, for E1.</p>},
  author       = {Eriksson, Anna L. and Perry, John R.B. and Coviello, Andrea D. and Delgado, Graciela E. and Ferrucci, Luigi and Hoffman, Andrew R. and Huhtaniemi, Ilpo T. and Ikram, M. Arfan and Karlsson, Magnus K. and Kleber, Marcus E. and Laughlin, Gail A. and Liu, Yongmei and Lorentzon, Mattias and Lunetta, Kathryn L. and Mellström, Dan and Murabito, Joanne M. and Murray, Anna and Nethander, Maria and Nielson, Carrie M. and Prokopenko, Inga and Pye, Stephen R. and Raffel, Leslie J. and Rivadeneira, Fernando and Srikanth, Priya and Stolk, Lisette and Teumer, Alexander and Travison, Thomas G. and Uitterlinden, André G. and Vaidya, Dhananjay and Vanderschueren, Dirk and Zmuda, Joseph M. and März, Winfried and Orwoll, Eric S. and Ouyang, Pamela and Vandenput, Liesbeth and Wu, Frederick C.W. and de Jong, Frank H. and Bhasin, Shalender and Kiel, Douglas P. and Ohlsson, Claes},
  issn         = {1945-7197},
  language     = {eng},
  month        = {03},
  number       = {3},
  pages        = {991--1004},
  publisher    = {The Endocrine Society},
  series       = {The Journal of clinical endocrinology and metabolism},
  title        = {Genetic Determinants of Circulating Estrogen Levels and Evidence of a Causal Effect of Estradiol on Bone Density in Men},
  url          = {http://dx.doi.org/10.1210/jc.2017-02060},
  volume       = {103},
  year         = {2018},
}