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Integrated flow-injection processing for on-line quantification of plasmid DNA during cultivation of E. coli

Nandakumar, M. P. ; Nordberg Karlsson, E. LU orcid and Mattiasson, B. LU (2001) In Biotechnology and Bioengineering 73(5). p.406-411
Abstract

An integrated flow-injection processing (FIP) system for the quantification of plasmids during cultivation is described. The system performs on-line sampling, cell lysis, and quantification of plasmids in an integrated manner during cultivation of E. coli. The system was operated by using a miniaturized expanded-bed column which can be used for handling samples containing cells and cell debris without interfering with the binding analysis. Two types of detectors (one measuring UV absorbance at 254 nm and a fluorometer) are used for on-line plasmid detection. The system was developed using standard solutions and it was successfully applied in monitoring plasmid contents during a cultivation of E. coli.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Escherichia coli, Flow-injection processing, On-line sampling, Plasmids
in
Biotechnology and Bioengineering
volume
73
issue
5
pages
6 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0035811187
  • pmid:11320511
ISSN
0006-3592
DOI
10.1002/bit.1074
language
English
LU publication?
yes
id
fe50666b-b6f9-4ca6-ab8b-d8d9d908b209
date added to LUP
2018-11-14 21:17:38
date last changed
2023-01-07 18:15:58
@article{fe50666b-b6f9-4ca6-ab8b-d8d9d908b209,
  abstract     = {{<p>An integrated flow-injection processing (FIP) system for the quantification of plasmids during cultivation is described. The system performs on-line sampling, cell lysis, and quantification of plasmids in an integrated manner during cultivation of E. coli. The system was operated by using a miniaturized expanded-bed column which can be used for handling samples containing cells and cell debris without interfering with the binding analysis. Two types of detectors (one measuring UV absorbance at 254 nm and a fluorometer) are used for on-line plasmid detection. The system was developed using standard solutions and it was successfully applied in monitoring plasmid contents during a cultivation of E. coli.</p>}},
  author       = {{Nandakumar, M. P. and Nordberg Karlsson, E. and Mattiasson, B.}},
  issn         = {{0006-3592}},
  keywords     = {{Escherichia coli; Flow-injection processing; On-line sampling; Plasmids}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{5}},
  pages        = {{406--411}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Biotechnology and Bioengineering}},
  title        = {{Integrated flow-injection processing for on-line quantification of plasmid DNA during cultivation of E. coli}},
  url          = {{http://dx.doi.org/10.1002/bit.1074}},
  doi          = {{10.1002/bit.1074}},
  volume       = {{73}},
  year         = {{2001}},
}