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Viability and survival of hNT neurons determine degree of functional recovery in grafted ischemic rats

Borlongan, C V; Saporta, S; Poulos, S G; Othberg, A LU and Sanberg, P R (1998) In NeuroReport 9(12). p.42-2837
Abstract

We recently reported behavioral improvements following intrastriatal transplantation of cryopreserved cultured human neuroteratocarcinoma-derived cells (hNT neurons) in rats with cerebral ischemia induced by occlusion of the middle cerebral artery. In the present study, the viability and survival of hNT neurons were evaluated immediately prior to the transplantation surgery and at 3 months post-transplantation in ischemic rats. Cryopreserved hNT neurons were routinely thawed, and trypan blue exclusion viability counts revealed 52-95% viable hNT neurons before transplantation. Monthly behavioral tests, starting at 1 month and extending to 3 months post-transplantation, revealed that ischemic animals that were intrastriatally transplanted... (More)

We recently reported behavioral improvements following intrastriatal transplantation of cryopreserved cultured human neuroteratocarcinoma-derived cells (hNT neurons) in rats with cerebral ischemia induced by occlusion of the middle cerebral artery. In the present study, the viability and survival of hNT neurons were evaluated immediately prior to the transplantation surgery and at 3 months post-transplantation in ischemic rats. Cryopreserved hNT neurons were routinely thawed, and trypan blue exclusion viability counts revealed 52-95% viable hNT neurons before transplantation. Monthly behavioral tests, starting at 1 month and extending to 3 months post-transplantation, revealed that ischemic animals that were intrastriatally transplanted with hNT neurons (approximately 40000) and treated with an immunosuppressive drug displayed normalization of asymmetrical motor behavior compared with ischemic animals that received medium alone. Within-subject comparisons of cell viability and subsequent behavioral changes revealed that a high cell viability just prior to transplantation surgery correlated highly with a robust and sustained functional improvement in the transplant recipient. Furthermore, histological analysis of grafted brains revealed a positive correlation between number of surviving hNT neurons and degree of functional recovery. In concert with similar reports on fetal tissue transplantation, we conclude that high cell viability is an important criterion for successful transplantation of cryopreserved neurons derived from cell lines to enhance graft-induced functional effects.

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published
subject
keywords
Animals, Brain Ischemia, Brain Neoplasms, Brain Tissue Transplantation, Cell Survival, Cell Transplantation, Humans, Male, Neoplasm Transplantation, Neostriatum, Neurons, Rats, Rats, Sprague-Dawley, Teratoma, Tumor Cells, Cultured, Journal Article, Research Support, Non-U.S. Gov't
in
NeuroReport
volume
9
issue
12
pages
42 - 2837
publisher
Lippincott Williams & Wilkins
external identifiers
  • Scopus:0032563590
ISSN
0959-4965
language
English
LU publication?
no
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feb2719a-e570-4e37-b718-947746a5d38e
date added to LUP
2016-12-11 09:56:19
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2017-01-01 08:42:55
@article{feb2719a-e570-4e37-b718-947746a5d38e,
  abstract     = {<p>We recently reported behavioral improvements following intrastriatal transplantation of cryopreserved cultured human neuroteratocarcinoma-derived cells (hNT neurons) in rats with cerebral ischemia induced by occlusion of the middle cerebral artery. In the present study, the viability and survival of hNT neurons were evaluated immediately prior to the transplantation surgery and at 3 months post-transplantation in ischemic rats. Cryopreserved hNT neurons were routinely thawed, and trypan blue exclusion viability counts revealed 52-95% viable hNT neurons before transplantation. Monthly behavioral tests, starting at 1 month and extending to 3 months post-transplantation, revealed that ischemic animals that were intrastriatally transplanted with hNT neurons (approximately 40000) and treated with an immunosuppressive drug displayed normalization of asymmetrical motor behavior compared with ischemic animals that received medium alone. Within-subject comparisons of cell viability and subsequent behavioral changes revealed that a high cell viability just prior to transplantation surgery correlated highly with a robust and sustained functional improvement in the transplant recipient. Furthermore, histological analysis of grafted brains revealed a positive correlation between number of surviving hNT neurons and degree of functional recovery. In concert with similar reports on fetal tissue transplantation, we conclude that high cell viability is an important criterion for successful transplantation of cryopreserved neurons derived from cell lines to enhance graft-induced functional effects.</p>},
  author       = {Borlongan, C V and Saporta, S and Poulos, S G and Othberg, A and Sanberg, P R},
  issn         = {0959-4965},
  keyword      = {Animals,Brain Ischemia,Brain Neoplasms,Brain Tissue Transplantation,Cell Survival,Cell Transplantation,Humans,Male,Neoplasm Transplantation,Neostriatum,Neurons,Rats,Rats, Sprague-Dawley,Teratoma,Tumor Cells, Cultured,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {08},
  number       = {12},
  pages        = {42--2837},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {NeuroReport},
  title        = {Viability and survival of hNT neurons determine degree of functional recovery in grafted ischemic rats},
  volume       = {9},
  year         = {1998},
}