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Dramatic influence of patchy attractions on short-time protein diffusion under crowded conditions

Bucciarelli, Saskia LU ; Myung, Jin Suk LU ; Farago, Bela; Das, Shibananda; Vliegenthart, Gerard A; Holderer, Olaf; Winkler, Roland G.; Schurtenberger, Peter LU ; Gompper, Gerhard and Stradner, Anna LU (2016) In Science advances 2(12). p.1601432-1601432
Abstract

In the dense and crowded environment of the cell cytoplasm, an individual protein feels the presence of and interacts with all surrounding proteins. While we expect this to strongly influence the short-time diffusion coefficient Ds of proteins on length scales comparable to the nearest-neighbor distance, this quantity is difficult to assess experimentally. We demonstrate that quantitative information about Ds can be obtained from quasi-elastic neutron scattering experiments using the neutron spin echo technique. We choose two well-characterized and highly stable eye lens proteins, bovine α-crystallin and γB-crystallin, and measure their diffusion at concentrations comparable to those present in the eye lens. While diffusion slows down... (More)

In the dense and crowded environment of the cell cytoplasm, an individual protein feels the presence of and interacts with all surrounding proteins. While we expect this to strongly influence the short-time diffusion coefficient Ds of proteins on length scales comparable to the nearest-neighbor distance, this quantity is difficult to assess experimentally. We demonstrate that quantitative information about Ds can be obtained from quasi-elastic neutron scattering experiments using the neutron spin echo technique. We choose two well-characterized and highly stable eye lens proteins, bovine α-crystallin and γB-crystallin, and measure their diffusion at concentrations comparable to those present in the eye lens. While diffusion slows down with increasing concentration for both proteins, we find marked variations that are directly linked to subtle differences in their interaction potentials. A comparison with computer simulations shows that anisotropic and patchy interactions play an essential role in determining the local short-time dynamics. Hence, our study clearly demonstrates the enormous effect that weak attractions can have on the short-time diffusion of proteins at concentrations comparable to those in the cellular cytosol.

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organization
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type
Contribution to journal
publication status
published
subject
in
Science advances
volume
2
issue
12
pages
9 pages
publisher
American Association for the Advancement of Science (AAAS)
external identifiers
  • wos:000391268800014
ISSN
2375-2548
DOI
10.1126/sciadv.1601432
language
English
LU publication?
yes
id
fefd8e86-f4f6-43dc-b416-cbfc1b9b8735
date added to LUP
2017-03-15 16:30:35
date last changed
2017-09-18 11:32:40
@article{fefd8e86-f4f6-43dc-b416-cbfc1b9b8735,
  abstract     = {<p>In the dense and crowded environment of the cell cytoplasm, an individual protein feels the presence of and interacts with all surrounding proteins. While we expect this to strongly influence the short-time diffusion coefficient Ds of proteins on length scales comparable to the nearest-neighbor distance, this quantity is difficult to assess experimentally. We demonstrate that quantitative information about Ds can be obtained from quasi-elastic neutron scattering experiments using the neutron spin echo technique. We choose two well-characterized and highly stable eye lens proteins, bovine α-crystallin and γB-crystallin, and measure their diffusion at concentrations comparable to those present in the eye lens. While diffusion slows down with increasing concentration for both proteins, we find marked variations that are directly linked to subtle differences in their interaction potentials. A comparison with computer simulations shows that anisotropic and patchy interactions play an essential role in determining the local short-time dynamics. Hence, our study clearly demonstrates the enormous effect that weak attractions can have on the short-time diffusion of proteins at concentrations comparable to those in the cellular cytosol.</p>},
  author       = {Bucciarelli, Saskia and Myung, Jin Suk and Farago, Bela and Das, Shibananda and Vliegenthart, Gerard A and Holderer, Olaf and Winkler, Roland G. and Schurtenberger, Peter and Gompper, Gerhard and Stradner, Anna},
  issn         = {2375-2548},
  language     = {eng},
  number       = {12},
  pages        = {1601432--1601432},
  publisher    = {American Association for the Advancement of Science (AAAS)},
  series       = {Science advances},
  title        = {Dramatic influence of patchy attractions on short-time protein diffusion under crowded conditions},
  url          = {http://dx.doi.org/10.1126/sciadv.1601432},
  volume       = {2},
  year         = {2016},
}