ANAE staining pattern of rat lymphocytes : lack of correlation with lymphocyte subclasses
(1981) In Scandinavian Journal of Immunology 14(5). p.7-523- Abstract
Lymphocytes from various lymphoid organs of WF and BN rats were isolated and stained for acid alpha-naphthyl acetate esterase (ANAE) activity. From all organs examined-peripheral blood, lymph nodes, and spleen-70-80% of the lymphocytes were stained for ANAE activity. After separation into B and non-B cells by affinity chromatography on anti-Ig columns, esterase-negative lymphocytes were detected in both populations, demonstrating that ANAE staining cannot be used as a lymphocyte subclass marker in rats, in contrast to the situation in human and mice.
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- author
- Malmström, P LU ; Jönsson, A and Sjögren, H O LU
- organization
- publishing date
- 1981-11
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, B-Lymphocytes/classification, Carboxylic Ester Hydrolases/metabolism, Chromatography, Affinity, Histocytochemistry, Lymph Nodes/cytology, Lymphocytes/classification, Naphthol AS D Esterase/metabolism, Rats, Rats, Inbred BN/immunology, Rats, Inbred WF/immunology, Spleen/cytology, Tissue Distribution
- in
- Scandinavian Journal of Immunology
- volume
- 14
- issue
- 5
- pages
- 7 - 523
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0019804287
- pmid:6977826
- ISSN
- 0300-9475
- language
- English
- LU publication?
- yes
- id
- ff185c0f-442b-4419-a935-faebf44fa0fc
- date added to LUP
- 2022-02-24 23:08:23
- date last changed
- 2024-01-04 23:14:47
@article{ff185c0f-442b-4419-a935-faebf44fa0fc, abstract = {{<p>Lymphocytes from various lymphoid organs of WF and BN rats were isolated and stained for acid alpha-naphthyl acetate esterase (ANAE) activity. From all organs examined-peripheral blood, lymph nodes, and spleen-70-80% of the lymphocytes were stained for ANAE activity. After separation into B and non-B cells by affinity chromatography on anti-Ig columns, esterase-negative lymphocytes were detected in both populations, demonstrating that ANAE staining cannot be used as a lymphocyte subclass marker in rats, in contrast to the situation in human and mice.</p>}}, author = {{Malmström, P and Jönsson, A and Sjögren, H O}}, issn = {{0300-9475}}, keywords = {{Animals; B-Lymphocytes/classification; Carboxylic Ester Hydrolases/metabolism; Chromatography, Affinity; Histocytochemistry; Lymph Nodes/cytology; Lymphocytes/classification; Naphthol AS D Esterase/metabolism; Rats; Rats, Inbred BN/immunology; Rats, Inbred WF/immunology; Spleen/cytology; Tissue Distribution}}, language = {{eng}}, number = {{5}}, pages = {{7--523}}, publisher = {{Wiley-Blackwell}}, series = {{Scandinavian Journal of Immunology}}, title = {{ANAE staining pattern of rat lymphocytes : lack of correlation with lymphocyte subclasses}}, volume = {{14}}, year = {{1981}}, }