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ANAE staining pattern of rat lymphocytes : lack of correlation with lymphocyte subclasses

Malmström, P LU ; Jönsson, A and Sjögren, H O LU (1981) In Scandinavian Journal of Immunology 14(5). p.7-523
Abstract

Lymphocytes from various lymphoid organs of WF and BN rats were isolated and stained for acid alpha-naphthyl acetate esterase (ANAE) activity. From all organs examined-peripheral blood, lymph nodes, and spleen-70-80% of the lymphocytes were stained for ANAE activity. After separation into B and non-B cells by affinity chromatography on anti-Ig columns, esterase-negative lymphocytes were detected in both populations, demonstrating that ANAE staining cannot be used as a lymphocyte subclass marker in rats, in contrast to the situation in human and mice.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, B-Lymphocytes/classification, Carboxylic Ester Hydrolases/metabolism, Chromatography, Affinity, Histocytochemistry, Lymph Nodes/cytology, Lymphocytes/classification, Naphthol AS D Esterase/metabolism, Rats, Rats, Inbred BN/immunology, Rats, Inbred WF/immunology, Spleen/cytology, Tissue Distribution
in
Scandinavian Journal of Immunology
volume
14
issue
5
pages
7 - 523
publisher
Wiley-Blackwell
external identifiers
  • pmid:6977826
  • scopus:0019804287
ISSN
0300-9475
language
English
LU publication?
yes
id
ff185c0f-442b-4419-a935-faebf44fa0fc
date added to LUP
2022-02-24 23:08:23
date last changed
2025-10-14 09:11:22
@article{ff185c0f-442b-4419-a935-faebf44fa0fc,
  abstract     = {{<p>Lymphocytes from various lymphoid organs of WF and BN rats were isolated and stained for acid alpha-naphthyl acetate esterase (ANAE) activity. From all organs examined-peripheral blood, lymph nodes, and spleen-70-80% of the lymphocytes were stained for ANAE activity. After separation into B and non-B cells by affinity chromatography on anti-Ig columns, esterase-negative lymphocytes were detected in both populations, demonstrating that ANAE staining cannot be used as a lymphocyte subclass marker in rats, in contrast to the situation in human and mice.</p>}},
  author       = {{Malmström, P and Jönsson, A and Sjögren, H O}},
  issn         = {{0300-9475}},
  keywords     = {{Animals; B-Lymphocytes/classification; Carboxylic Ester Hydrolases/metabolism; Chromatography, Affinity; Histocytochemistry; Lymph Nodes/cytology; Lymphocytes/classification; Naphthol AS D Esterase/metabolism; Rats; Rats, Inbred BN/immunology; Rats, Inbred WF/immunology; Spleen/cytology; Tissue Distribution}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{7--523}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Scandinavian Journal of Immunology}},
  title        = {{ANAE staining pattern of rat lymphocytes : lack of correlation with lymphocyte subclasses}},
  volume       = {{14}},
  year         = {{1981}},
}