Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Synthetic protease inhibitors and post-ejaculatory degradation of human semen proteins

Lilja, H. LU orcid and Weiber, H. LU (1984) In Scandinavian Journal of Clinical and Laboratory Investigation 44(5). p.433-438
Abstract

Normal post-ejaculatory proteolytic changes in human seminal plasma rapidly distort its electrophoretic protein pattern. This invalidates the electrophoretic evaluation of the content in the secretion from the accessory sex glands. Both agarose and sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) were used to study the proteolytic changes and how they could be modified by various synthetic protease inhibitors. Liquefaction of coagulated semen could be inhibited by adding o-phenanthroline directly after ejaculation, whereas neither neutrally buffered Na2EDTA, di-isopropylfluorophosphate (DFP), benzamidine, nor thiol reagents proved effective. Addition of the serine protease inhibitors DFP and benzamidine,... (More)

Normal post-ejaculatory proteolytic changes in human seminal plasma rapidly distort its electrophoretic protein pattern. This invalidates the electrophoretic evaluation of the content in the secretion from the accessory sex glands. Both agarose and sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) were used to study the proteolytic changes and how they could be modified by various synthetic protease inhibitors. Liquefaction of coagulated semen could be inhibited by adding o-phenanthroline directly after ejaculation, whereas neither neutrally buffered Na2EDTA, di-isopropylfluorophosphate (DFP), benzamidine, nor thiol reagents proved effective. Addition of the serine protease inhibitors DFP and benzamidine, o-phenanthroline, and iodoacetamide substantially retarded the proteolytic alterations of the proteins as demonstrated by both agarose electrophoresis and SDS-PAGE. We recommend that electrophoretic protein analysis of human semen be performed on ejaculates collected in vessels containing protease inhibitors. For routine analysis, the addition of benzamidine ensures sufficiently stable proteins to permit reliable electrophoretic analysis of samples stored at room temperature for 4 h.

(Less)
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Prostate, Protease inhibitors, Protein degradation, Semen, Seminal vesicles
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
44
issue
5
pages
433 - 438
publisher
Informa Healthcare
external identifiers
  • pmid:6484483
  • scopus:0021183512
ISSN
0036-5513
DOI
10.3109/00365518409083834
language
English
LU publication?
yes
id
ff1a9eed-24a8-4638-a2da-392385df13d4
date added to LUP
2022-12-06 16:56:46
date last changed
2024-01-03 19:31:05
@article{ff1a9eed-24a8-4638-a2da-392385df13d4,
  abstract     = {{<p>Normal post-ejaculatory proteolytic changes in human seminal plasma rapidly distort its electrophoretic protein pattern. This invalidates the electrophoretic evaluation of the content in the secretion from the accessory sex glands. Both agarose and sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) were used to study the proteolytic changes and how they could be modified by various synthetic protease inhibitors. Liquefaction of coagulated semen could be inhibited by adding o-phenanthroline directly after ejaculation, whereas neither neutrally buffered Na<sub>2</sub>EDTA, di-isopropylfluorophosphate (DFP), benzamidine, nor thiol reagents proved effective. Addition of the serine protease inhibitors DFP and benzamidine, o-phenanthroline, and iodoacetamide substantially retarded the proteolytic alterations of the proteins as demonstrated by both agarose electrophoresis and SDS-PAGE. We recommend that electrophoretic protein analysis of human semen be performed on ejaculates collected in vessels containing protease inhibitors. For routine analysis, the addition of benzamidine ensures sufficiently stable proteins to permit reliable electrophoretic analysis of samples stored at room temperature for 4 h.</p>}},
  author       = {{Lilja, H. and Weiber, H.}},
  issn         = {{0036-5513}},
  keywords     = {{Prostate; Protease inhibitors; Protein degradation; Semen; Seminal vesicles}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{433--438}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
  title        = {{Synthetic protease inhibitors and post-ejaculatory degradation of human semen proteins}},
  url          = {{http://dx.doi.org/10.3109/00365518409083834}},
  doi          = {{10.3109/00365518409083834}},
  volume       = {{44}},
  year         = {{1984}},
}